Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Immunologic Research
Published in: Immunologic Research 1-3/2010

01-07-2010

Class I MHC molecules as probes of membrane patchiness: from biophysical measurements to modulation of immune responses

Author: Michael Edidin

Published in: Immunologic Research | Issue 1-3/2010

Login to get access

Abstract

Here I summarize decades of work using the biophysics of class I MHC molecules to probe the patchiness and heterogeneity of cell surfaces. This program began as a study of membranes generally. MHC molecules were a convenient probe. However, in recent years, it has become clear that the lateral distribution, clustering, of class I MHC molecules in the membrane affects their recognition by effector CTL. This offers the possibility of enhancing or reducing T-cell recognition of targets by altering the clustering of their membrane proteins.
Literature
1.
Watkins JF, Grace DM. Studies on the surface antigens of interspecific mammalian cell heterokaryons. J Cell Sci. 1967;2:193–204.PubMed
2.
Frye LD, Edidin M. The rapid intermixing of cell surface antigens after formation of mouse-human heterokaryons. J Cell Sci. 1970;7:319–35.PubMed
3.
Jacobson K, Wu E, Poste G. Measurement of the translational mobility of concanavalin A in glycerol-saline solutions and on the cell surface by fluorescence recovery after photobleaching. Biochim Biophys Acta. 1976;433:215–22.CrossRefPubMed
4.
Schlessinger J, Koppel DE, Axelrod D, Jacobson K, Webb WW, Elson EL. Lateral transport on cell membranes: mobility of concanavalin A receptors on myoblasts. Proc Natl Acad Sci USA. 1976;73:2409–13.CrossRefPubMed
5.
Zagyansky Y, Edidin M. Lateral diffusion of concanavalin A receptors in the plasma membrane of mouse fibroblasts. Biochim Biophys Acta. 1976;433:209–14.CrossRefPubMed
6.
Edidin M, Zagyansky Y, Lardner TJ. Measurement of membrane protein lateraldiffusion in single cells. Science. 1976;191:466–8.CrossRefPubMed
7.
Wier M, Edidin M. Constraint of the translational diffusion of a membrane glycoprotein by its external domains. Science. 1988;242:412–4.CrossRefPubMed
8.
Yechiel E, Edidin M. Micrometer-scale domains in fibroblast plasma membranes. J Cell Biol. 1987;105:755–60.CrossRefPubMed
9.
Edidin M, Stroynowski I. Differences between the lateral organization of conventional and inositol phospholipid-anchored membrane proteins. A further definition of micrometer scale membrane domains. J Cell Biol. 1991;112:1143–50.CrossRefPubMed
10.
Edidin M, Zuniga M. Lateral diffusion of wild-type and mutant Ld antigens in L cells. J Cell Biol. 1984;99:2333–5.CrossRefPubMed
11.
Schnapp BJ, Gelles J, Sheetz MP. Nanometer-scale measurements using video light microscopy. Cell Motil Cytoskeleton. 1988;10:47–53.CrossRefPubMed
12.
Saxton MJ, Jacobson K. Single-particle tracking: applications to membrane dynamics. Annu Rev Biophys Biomol Struct. 1997;26:373–99.CrossRefPubMed
13.
Edidin M, Zuñiga MC, Sheetz MP. Truncation mutants define and locate cytoplasmic barriers to lateral mobility of membrane glycoproteins. Proc Natl Acad Sci USA. 1994;91:3378–82.CrossRefPubMed
14.
Capps GG, Pine S, Edidin M, Zuñiga MC. Short class I major histocompatibility complex cytoplasmic tails differing in charge detect arbiters of lateral diffusion in the plasma membrane. Biophys J. 2004;86:2896–909.CrossRefPubMed
15.
16.
Matko J, Bushkin Y, Wei T, Edidin M. Clustering of class I HLA molecules on the surfaces of activated and transformed human cells. J Immunol. 1994;152:3353–60.PubMed
17.
Chakrabarti A, Matko J, Rahman NA, Barisas BG, Edidin M. Self-association of class I major histocompatibility complex molecules in liposome and cell surface membranes. Biochemistry. 1992;31:7182–9.CrossRefPubMed
18.
Edidin M. Near-field scanning optical microscopy, a siren call to biology. Traffic. 2001;2:797–803.CrossRefPubMed
19.
Hwang J, Gheber LA, Margolis L, Edidin M. Domains in cell plasma membranes investigated by near-field scanning optical microscopy. Biophys J. 1998;74:2184–90.CrossRefPubMed
20.
Tang Q, Edidin M. Vesicle trafficking and cell surface membrane patchiness. Biophys J. 2001;81:196–203.CrossRefPubMed
21.
Gheber LA, Edidin M. A model for membrane patchiness: lateral diffusion in the presence of barriers and vesicle traffic. Biophys J. 1999;77:3163–75.CrossRefPubMed
22.
23.
McKeithan TW. Kinetic proofreading in T-cell receptor signal transduction. Proc Natl Acad Sci USA. 1995;92:5042–6.CrossRefPubMed
24.
San José E, Borroto A, Niedergang F, Alcover A, Alarcón B. Triggering the TCR complex causes the downregulation of nonengaged receptors by a signal transduction-dependent mechanism. Immunity. 2000;12:161–70.CrossRefPubMed
25.
Bachmann MF, Ohashi PS. The role of T-cell receptor dimerization in T-cell activation. Immunol Today. 1999;20:568–76.CrossRefPubMed
26.
Bodnár A, Bacsó Z, Jenei A, Jovin TM, Edidin M, Damjanovich S, et al. Class I HLA oligomerization at the surface of B cells is controlled by exogenous beta(2)-microglobulin: implications in activation of cytotoxic T lymphocytes. Int Immunol. 2003;15:331–9.CrossRefPubMed
27.
Edidin M. The state of lipid rafts: from model membranes to cells. Annu Rev Biophys Biomol Struct. 2003;32:257–83.CrossRefPubMed
28.
Kwik J, Boyle S, Fooksman D, Margolis L, Sheetz MP, Edidin M. Membrane cholesterol, lateral mobility, and the phosphatidylinositol 4, 5-bisphosphate-dependent organization of cell actin. Proc Natl Acad Sci USA. 2003;100:13964–9.CrossRefPubMed
29.
Fooksman DR, Grönvall GK, Tang Q, Edidin M. Clustering class I MHC modulates sensitivity of T cell recognition. J Immunol. 2006;176:6673–80.PubMed
30.
Neumann F, Sturm C, Hulsmeyer M, Dauth N, Guillaume P, Luescher IF, et al. Fab antibodies capable of blocking T cells by competitive binding have the identical specificity but a higher affinity to the MHC-peptide-complex than the T cell receptor. Immunol Lett. 2009;125:86–92.CrossRefPubMed
31.
Hosseini BH, Louban I, Djandji D, Wabnitz GH, Deeg J, Bulbuc N, et al. Immune synapse formation determines interaction forces between T cells and antigen-presenting cells measured by atomic force microscopy. Proc Natl Acad Sci USA. 2009;106:17852–7.CrossRefPubMed
32.
Shen K, Thomas VK, Dustin ML, Kam LC. Micropatterning of costimulatory ligands enhances CD4+T cell function. Proc Natl Acad Sci USA. 2008;105:7791–6.CrossRefPubMed
33.
Lippincott-Schwartz J, Snapp E, Kenworthy A. Studying protein dynamics in living cells. Nat Rev Mol Cell Biol. 2001;2:444–56.CrossRefPubMed
34.
Kwik, JF. Immobilization of MHC class I molecules enhances allogeneic target cell lysis by CD8+T cells. Ph.D. Thesis, Johns Hopkins University; 2000.
35.
Fooksman, DR. The surface organization of MHC class I molecules regulates their recognition by T-cells. Ph.D. Thesis, Johns Hopkins University; 2007.
Metadata
Title
Class I MHC molecules as probes of membrane patchiness: from biophysical measurements to modulation of immune responses
Author
Michael Edidin
Publication date
01-07-2010
Publisher
Humana Press Inc
Published in
Immunologic Research / Issue 1-3/2010
Print ISSN: 0257-277X
Electronic ISSN: 1559-0755
DOI
https://doi.org/10.1007/s12026-009-8159-9

Other articles of this Issue 1-3/2010

Immunologic Research 1-3/2010 Go to the issue

EditorialNotes

Immunologic research at Johns Hopkins University: introduction and overview

OriginalPaper

Controlling somatic hypermutation in immunoglobulin variable and switch regions

OriginalPaper

Immune regulation in the retina

OriginalPaper

Reconstitution of self-tolerance after hematopoietic stem cell transplantation

OriginalPaper

Analysis of gene profile, steady state proliferation and apoptosis of double-negative T cells in the periphery and gut epithelium provides new insights into the biological functions of the Fas pathway

OriginalPaper

Recovery from viral encephalomyelitis: immune-mediated noncytolytic virus clearance from neurons