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Endocrine

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01-07-2019 | Insulins | Original Article

Phenotypic characterization of a novel type 2 diabetes animal model in a SHANXI MU colony of Chinese hamsters

Authors: Lu Wang, Chenyang Wang, Ruihu Zhang, Yu Liu, Chunfang Wang, Guohua Song, Jingjing Yu, Zhaoyang Chen

Published in: Endocrine | Issue 1/2019

Abstract

Purpose

Developing animal models for human diseases is critical for studying complex diseases such as type 2 diabetes mellitus (T2DM). Since inbred colonies of Chinese hamsters tend toward spontaneous development of diabetes, we investigated them as a possible model.

Methods

We regarded individuals with fasting blood glucose (FBG) higher than 6.0 mmol/L and post-prandial blood glucose (PBG) higher than 7.0 mmol/L as diabetic based on the mean and 95% frequency distribution values of FBG and PBG. Diabetic hamsters were characterized based on metabolic profiles, histopathological features, and changes in the expression of genes involved in glucose and lipid metabolism.

Results

Metabolic analyses showed that diabetic hamsters exhibited mild hyperglycemia, hypertriglyceridemia, glucose intolerance, and insulin resistance. Histopathological analysis revealed that cell nuclei migrated inward in skeletal muscle and obvious partial liver lipid deposition and focal necrosis was found. We additionally observed mild injury, atrophy, and occasional vacuolization in islet cells. Changes in the expression of several genes related to glucose and lipid metabolism were observed. Decreased expression of adiponectin and GLUT4 and increased expression of PPARγ, Akt, and leptin was observed in skeletal muscle. Decreased expression of adiponectin with increased expression of PPARγ and leptin was observed in the liver.

Conclusions

These results indicate that we have established a spontaneous diabetic hamster line that closely mimics human T2DM, which may hold potential for further research on the pathogenesis and treatment of this disease.

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Title: Phenotypic characterization of a novel type 2 diabetes animal model in a SHANXI MU colony of Chinese hamsters
Authors: Lu Wang
Chenyang Wang
Ruihu Zhang
Yu Liu
Chunfang Wang
Guohua Song
Jingjing Yu
Zhaoyang Chen
Publication date: 01-07-2019
Publisher: Springer US
Keywords: Insulins
Diabetes
Insulins
Type 2 Diabetes
Published in: Endocrine / Issue 1/2019
Print ISSN: 1355-008X
Electronic ISSN: 1559-0100
DOI: https://doi.org/10.1007/s12020-019-01940-x

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Phenotypic characterization of a novel type 2 diabetes animal model in a SHANXI MU colony of Chinese hamsters

Abstract

Purpose

Methods

Results

Conclusions

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Abstract

Purpose

Methods

Results

Conclusions

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