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01-05-2017 | Original Article

Glibenclamide treatment blocks metabolic dysfunctions and improves vagal activity in monosodium glutamate-obese male rats

Authors: Claudinéia C. S. Franco, Kelly V. Prates, Carina Previate, Ana M. P. Moraes, Camila C. I. Matiusso, Rosiane A. Miranda, Júlio C. de Oliveira, Laize P. Tófolo, Isabela P. Martins, Luiz F. Barella, Tatiane A. Ribeiro, Ananda Malta, Audrei Pavanello, Flávio A. Francisco, Rodrigo M. Gomes, Vander S. Alves, Veridiana M. Moreira, Késia P. Rigo, Douglas L. Almeida, Juliane R. de Sant Anna, Marialba A. A. C. Prado, Paulo C. F. Mathias

Published in: Endocrine | Issue 2/2017

Abstract

Background/Aims

Autonomic nervous system imbalance is associated with metabolic diseases, including diabetes. Glibenclamide is an antidiabetic drug that acts by stimulating insulin secretion from pancreatic beta cells and is widely used in the treatment of type 2 diabetes. Since there is scarce data concerning autonomic nervous system activity and diabetes, the aim of this work was to test whether glibenclamide can improve autonomic nervous system activity and muscarinic acetylcholine receptor function in pre-diabetic obese male rats.

Methods

Pre-diabetes was induced by treatment with monosodium L-glutamate in neonatal rats. The monosodium L-glutamate group was treated with glibenclamide (2 mg/kg body weight /day) from weaning to 100 days of age, and the control group was treated with water. Body weight, food intake, Lee index, fasting glucose, insulin levels, homeostasis model assessment of insulin resistance, omeostasis model assessment of β-cell function, and fat tissue accumulation were measured. The vagus and sympathetic nerve electrical activity were recorded. Insulin secretion was measured in isolated islets challenged with glucose, acetylcholine, and the selective muscarinic acetylcholine receptor antagonists by radioimmunoassay technique.

Results

Glibenclamide treatment prevented the onset of obesity and diminished the retroperitoneal (18%) and epididymal (25%) fat pad tissues. In addition, the glibenclamide treatment also reduced the parasympathetic activity by 28% and glycemia by 20% in monosodium L-glutamate-treated rats. The insulinotropic effect and unaltered cholinergic actions in islets from monosodium L-glutamate groups were increased.

Conclusion

Early glibenclamide treatment prevents monosodium L-glutamate-induced obesity onset by balancing autonomic nervous system activity.

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Title: Glibenclamide treatment blocks metabolic dysfunctions and improves vagal activity in monosodium glutamate-obese male rats
Authors: Claudinéia C. S. Franco
Kelly V. Prates
Carina Previate
Ana M. P. Moraes
Camila C. I. Matiusso
Rosiane A. Miranda
Júlio C. de Oliveira
Laize P. Tófolo
Isabela P. Martins
Luiz F. Barella
Tatiane A. Ribeiro
Ananda Malta
Audrei Pavanello
Flávio A. Francisco
Rodrigo M. Gomes
Vander S. Alves
Veridiana M. Moreira
Késia P. Rigo
Douglas L. Almeida
Juliane R. de Sant Anna
Marialba A. A. C. Prado
Paulo C. F. Mathias
Publication date: 01-05-2017
Publisher: Springer US
Published in: Endocrine / Issue 2/2017
Print ISSN: 1355-008X
Electronic ISSN: 1559-0100
DOI: https://doi.org/10.1007/s12020-017-1263-z

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Glibenclamide treatment blocks metabolic dysfunctions and improves vagal activity in monosodium glutamate-obese male rats

Abstract

Background/Aims

Methods

Results

Conclusion

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Abstract

Background/Aims

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Conclusion

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