Top

Published in:

Open Access 01-09-2018 | Original Paper

CD271+, CXCR7+, CXCR4+, and CD133+ Stem/Progenitor Cells and Clinical Characteristics of Acute Ischemic Stroke Patients

Authors: Anna Gójska-Grymajło, Maciej Zieliński, Dariusz Gąsecki, Kamil Kowalczyk, Mariusz Kwarciany, Barbara Seroczyńska, Walenty M. Nyka

Published in: NeuroMolecular Medicine | Issue 3/2018

Abstract

Ischemic stroke causes mobilization of various groups of progenitor cells from bone marrow to bloodstream and this correlates with the neurological status of stroke patients. The goal of our study was to identify the activity of chosen progenitor/stem cells in the peripheral blood of acute ischemic stroke patients in the first 7 days after the incident, through associations between the levels of the cells and clinical features of the patients. Thirty-three acute ischemic stroke patients and 15 non-stroke control subjects had their venous blood collected repeatedly in order to assess the levels of the CD45–CD34 + CD271+, the CD45–CD34 + CXCR4+, the CD45–CD34 + CXCR7+, and the CD45–CD34 + CD133+ stem/progenitor cells by means of flow cytometry. The patients underwent repeated neurological and clinical assessments, pulse wave velocity (PWV) assessment on day 5, and MRI on day 1 and 5 ± 2. The levels of the CD45–CD34 + CXCR7+ and the CD45–CD34 + CD271+ cells were lower in the stroke patients compared with the control subjects. Only the CD45–CD34 + CD271+ cells correlated positively with lesion volume in the second MRI. The levels of the CD45–CD34 + CD133+ cells on day 2 correlated negatively with PWV and NIHSS score on day 9. The patients whose PWV was above 10 m/s had significantly higher levels of the CD45–CD34 + CXCR4+ and the CD45–CD34 + CXCR7+ cells on day 1 than those with PWV below 10 m/s. This study discovers possible activity of the CD45–CD34 + CD271+ progenitor/stem cells during the first 7 days after ischemic stroke, suggests associations of the CD45–CD34 + CD133+ cells with the neurological status of stroke patients, and some activity of the CD45–CD34 + CD133+, the CD45–CD34 + CXCR4+, and the CD45–CD34 + CXCR7+ progenitor/stem cells in the process of arterial remodeling.

Available only for authorised users

Bakondi, B., Shimada, I. S., Perry, A., Munoz, J. R., Ylostalo, J., Howard, A. B., et al. (2009). CD133 identifies a human bone marrow stem/progenitor cell sub-population with a repertoire of secreted factors that protect against stroke. Molecular Therapy, 17(11), 1938–1947.PubMedPubMedCentralCrossRef

Bang, O. Y., Kim, E. H., Cha, J. M., & Moon, G. J. (2016). Adult Stem Cell Therapy for Stroke: Challenges and Progress. Journal of Stroke, 18(3), 256–266.PubMedPubMedCentralCrossRef

Bao, J., Zhu, J., Luo, S., Cheng, Y., & Zhou, S. (2016). CXCR7 suppression modulates microglial chemotaxis to ameliorate experimentally-induced autoimmune encephalomyelitis. Biochemical and Biophysical Research Communications, 469(1), 1–7.PubMedCrossRef

Beer, L., Mildner, M., Gyöngyösi, M., & Ankersmit, H. J. (2016). Peripheral blood mononuclear cell secretome for tissue repair. Apoptosis, 21(12), 1–18.CrossRef

Blanchet, X., Langer, M., Weber, C., Koenen, R., & von Hundelshausen, P. (2012). Touch of chemokines. Frontiers in Immunology, 3, 175.PubMedPubMedCentralCrossRef

Bogoslovsky, T., Chaudhry, A., Latour, L., Maric, D., Luby, M., Spatz, M., et al. (2010). Endothelial progenitor cells correlate with lesion volume and growth in acute stroke. Neurology, 75(23), 2059–2062.PubMedPubMedCentralCrossRef

Boiko, A. D., Razorenova, O. V., van de Rijn, M., Swetter, S. M., Johnson, D. L., Ly, D. P., et al. (2010). Human melanoma-initiating cells express neural crest nerve growth factor receptor CD271. Nature, 466(7302), 133–137.PubMedPubMedCentralCrossRef

Cao, Z., Lis, R., Ginsberg, M., Chavez, D., Shido, K., Rabbany, S. Y., et al. (2016). Targeting of the pulmonary capillary vascular niche promotes lung alveolar repair and ameliorates fibrosis. Nature Medicine, 22(2), 154–162.PubMedPubMedCentralCrossRef

Carvalho, I. M., Coelho, P. B., Costa, P. C., Marques, C. S., Oliveira, R. S., & Ferreira, D. C. (2015). Current neurogenic and neuroprotective strategies to prevent and treat neurodegenerative and neuropsychiatric disorders. NeuroMolecular Medicine, 17(4), 404–422.PubMedCrossRef

Chen, J., Chen, J., Chen, S., Zhang, C., Zhang, L., Xiao, X., et al. (2012). Transfusion of CXCR4-primed endothelial progenitor cells reduces cerebral ischemic damage and promotes repair in db/db diabetic mice. PLoS ONE, 7(11), e50105.PubMedPubMedCentralCrossRef

Dai, X., Yan, X., Zeng, J., Chen, J., Wang, Y., Chen, J., et al. (2017). Elevating CXCR7 improves angiogenic function of EPCs via Akt/GSK-3β/Fyn-mediated Nrf2 activation in diabetic limb ischemia. Circulation Research, 120(5), e7–e23.PubMedPubMedCentralCrossRef

Demir, I. E., Kujundzic, K., Pfitzinger, P. L., Saricaoglu, ÖC., Teller, S., Kehl, T., et al. (2017). Early pancreatic cancer lesions suppress pain through CXCL12-mediated chemoattraction of Schwann cells. Proceedings of the National Academy of Sciences, 114(1), e85–e94.CrossRef

Deng, L., Zheng, W., Dong, X., Liu, J., Zhu, C., Lu, D., et al. (2017). Chemokine receptor CXCR7 is an independent prognostic biomarker in glioblastoma. Cancer Biomarkers, 20(1), 1–6.PubMedCrossRef

Dunac, A., Frelin, C., Popolo-Blondeau, M., Chatel, M., Mahagne, M. H., & Philip, P. J. M. (2007). Neurological and functional recovery in human stroke are associated with peripheral blood CD34+ cell mobilization. Journal of Neurology, 254(3), 327–332.PubMedCrossRef

Fadini, G. P., Coracina, A., Baesso, I., Agostini, C., Tiengo, A., Avogaro, A., & De Kreutzenberg, S. V. (2006). Peripheral blood CD34 + KDR + endothelial progenitor cells are determinants of subclinical atherosclerosis in a middle-aged general population. Stroke, 37(9), 2277–2282.PubMedCrossRef

Gąsecki, D., Rojek, A., Kwarciany, M., Kubach, M., Boutouyrie, P., Nyka, W., et al. (2012). Aortic stiffness predicts functional outcome in patients after ischemic stroke. Stroke, 43(2), 543–544.PubMedCrossRef

Gójska-Grymajło, A., Nyka, W. M., Zieliński, M., & Jakubowski, Z. (2012). CD34/CXCR4 stem cell dynamics in acute stroke patients. Folia Neuropathologica, 50, 140–146.PubMed

Goto, M., Yoshida, T., Yamamoto, Y., Furukita, Y., Inoue, S., Fujiwara, S., et al. (2017). CXCR4 expression is associated with poor prognosis in patients with esophageal squamous cell carcinoma. Annals of Surgical Oncology, 24(3), 832–840.PubMedCrossRef

Gu, H., Zhang, Z., Zhang, Z. B., Wang, Q. Q., Xi, X. W., & He, Y. Y. (2017). The role of the SDF-1/ CXCR7 axis on the growth and invasion ability of endometrial cancer cells. Archives of Gynecology and Obstetrics, 295(4), 987–995.PubMedCrossRef

Guo, R., Fierro-Fine, A., Goddard, L., Russell, M., Chen, J., Liu, C. Z., et al. (2014). Increased expression of melanoma stem cell marker CD271 in metastatic melanoma to the brain. International Journal of Clinical and Experimental Pathology, 7(12), 8947–8951.PubMedPubMedCentral

Hao, H., Hu, S., Chen, H., Bu, D., Zhu, L., Xu, C., et al. (2017). Loss of endothelial CXCR7 impairs vascular homeostasis and cardiac remodeling after myocardial infarction: Implications for cardiovascular drug discovery. Circulation, 135(13), 1253–1264.PubMedCrossRef

Hennemann, B., Ickenstein, G., Sauerbruch, S., Luecke, K., Haas, S., Horn, M., et al. (2008). Mobilization of CD34+ hematopoietic cells, colony-forming cells and long-term culture-initiating cells into the peripheral blood of patients with an acute cerebral ischemic insult. Cytotherapy, 10(3), 303–311.PubMedCrossRef

Huang, Y., Ye, Y., Long, P., Zhao, S., Zhang, L., & Yanni, A. (2017). Silencing of CXCR4 and CXCR7 expression by RNA interference suppresses human endometrial carcinoma growth in vivo. American Journal of Translational Research, 9(4), 1896–1904.PubMedPubMedCentral

Iso, Y., Yamaya, S., Sato, T., Poole, C. N., Isoyama, K., Mimura, M., et al. (2012). Distinct mobilization of circulating CD271 mesenchymal progenitors from hematopoietic progenitors during aging and after myocardial infarction. Stem Cells Translarional Medicine, 1(6), 462–468.CrossRef

Kwarciany, M., Gąsecki, D., Kowalczyk, K., Rojek, A., Laurent, S., Boutouyrie, P., et al. (2016). Acute hypertensive response in ischemic stroke is associated with increased aortic stiffness. Atherosclerosis, 251, 1–5.PubMedCrossRef

Laurent, S., Cockcroft, J., Van Bortel, L., Boutouyrie, P., Giannattasio, C., Hayoz, D., et al. (2006). Expert consensus document on arterial stiffness: Methodological issues and clinical applications. European Heart Journal, 27(21), 2588–2605.PubMedCrossRef

Li, X., Zhu, M., Penfold, M. E., Koenen, R. R., Thiemann, A., Heyll, K., et al. (2014). Activation of CXCR7 limits atherosclerosis and improves hyperlipidemia by increasing cholesterol uptake in adipose tissue. Circulation, 129(11), 1244–1253.PubMedCrossRef

Liu, Y., Carson-Walter, E., & Walter, K. A. (2014). Chemokine receptor CXCR7 is a functional receptor for CXCL12 in brain endothelial cells. PLoS ONE, 9(8), 4–12.

Madhavan, L., & Collier, T. J. (2010). A synergistic approach for neural repair: Cell transplantation and induction of endogenous precursor cell activity. Neuropharmacology, 58(6), 835–844.PubMedCrossRef

Marketou, M. E., Kalyva, A., Parthenakis, F. I., Pontikoglou, C., Maragkoudakis, S., Kontaraki, J. E., et al. (2014). Circulating endothelial progenitor cells in hypertensive patients with increased arterial stiffness. Journal of Clinical Hypertension (Greenwich, Conn.), 16(4), 295–300.CrossRef

Mirabelli-Badenier, M., Braunersreuther, V., Viviani, G. L., Dallegri, F., Quercioli, A., Veneselli, E., et al. (2011). March). CC and CXC chemokines are pivotal mediators of cerebral injury in ischaemic stroke. Thrombosis and Haemostasis, 105(3), 409–420.PubMedCrossRef

Nambara, S., Iguchi, T., Oki, E., Tan, P., Maehara, Y., & Mimori, K. (2017). Overexpression of CXCR7 Is a novel prognostic indicator in gastric cancer. Digestive Surgery, 34(4), 312–318.PubMedCrossRef

Paczkowska, E., Larysz, B., Rzeuski, R., Karbicka, A., Jaławiński, R., Kornacewicz-Jach, Z., et al. (2005). Human hematopoietic stem/progenitor-enriched CD34 + cells are mobilized into peripheral blood during stress related to ischemic stroke or acute myocardial infarction. European Journal of Haematology, 75(6), 461–467.PubMedCrossRef

Paczkowska, E., Piecyk, K., Łuczkowska, K., Kotowski, M., Rogińska, D., Pius-Sadowska, E., et al. (2016). Expression of neurotrophins and their receptors in human CD34⁺ bone marrow cells. Journal of Physiology and Pharmacology, 67(1), 151–159.PubMed

Quirici, N., Scavullo, C., de Girolamo, L., Lopa, S., Arrigoni, E., Deliliers, G. L., & Brini, A. T. (2010). Anti-L-NGFR and -CD34 monoclonal antibodies identify multipotent mesenchymal stem cells in human adipose tissue. Stem Cells and Development, 19(6), 915–925.PubMedCrossRef

Sanchez-Martin, L., Sanchez-Mateos, P., & Cabanas, C. (2013). CXCR7 impact on CXCL12 biology and disease. Trends in Molecular Medicine, 19(1), 12–22.PubMedCrossRef

Schonemeier, B., Schulz, S., Hoellt, V., & Stumm, R. (2008). Enhanced expression of the CXCl12/SDF-1 chemokine receptor CXCR7 after cerebral ischemia in the rat brain. Journal of Neuroimmunology, 198(1–2), 39–45.PubMedCrossRef

Shimada, I. S., & Spees, J. L. (2011). Stem and progenitor cells for neurological repair: Minor issues, major hurdles, and exciting opportunities for paracrine-based therapeutics. Journal of Cellular Biochemistry, 112(2), 374–380.PubMedPubMedCentralCrossRef

Sobrino, T., Hurtado, O., Moro, M., Rodríguez-Yáñez, M., Castellanos, M., Brea, D., et al. (2007). The increase of circulating endothelial progenitor cells after acute ischemic stroke is associated with good outcome. Stroke, 38(10), 2759–2764.PubMedCrossRef

Taguchi, A., Matsuyama, T., Moriwaki, H., Hayashi, T., Hayashida, K., Nagatsuka, K., et al. (2004). Circulating CD34-positive cells provide an index of cerebrovascular function. Circulation, 109(24), 2972–2975.PubMedCrossRef

Tarnowski, M., Liu, R., Wysoczyński, M., Ratajczak, J., Kucia, M., & Ratajczak, M. Z. (2010). CXCR7: A new SDF-1-binding receptor in contrast to normal CD34+ progenitors is functional and is expressed at higher level in human malignant hematopoietic cells. European Journal of Haematology, 85(6), 472–483.PubMedCrossRef

Tian, J., Li, X., Si, M., Liu, T., & Li, J. (2014). CD271+ osteosarcoma cells display stem-like properties. PLoS ONE, 9(6), e98549.PubMedPubMedCentralCrossRef

Wang, Y., Fu, W., Zhang, S., He, X., Liu, Z., Gao, D., & Xu, T. (2014). CXCR-7 receptor promotes SDF-1α-induced migration of bone marrow mesenchymal stem cells in the transient cerebral ischemia/reperfusion rat hippocampus. Brain Research, 1575(1), 78–86.PubMedCrossRef

Zernecke, A., Shagdarsuren, E., & Weber, C. (2008). Chemokines in atherosclerosis an update. Arteriosclerosis, Thrombosis, and Vascular Biology, 28(11), 1897–1908.PubMedCrossRef

Zhang, X. Y., Su, C., Cao, Z., Xu, S. Y., Xia, W. H., Xie, W. L., et al. (2014). CXCR7 upregulation is required for early endothelial progenitor cell-mediated endothelial repair in patients with hypertension. Hypertension, 63(2), 383–389.PubMedCrossRef

Title: CD271+, CXCR7+, CXCR4+, and CD133+ Stem/Progenitor Cells and Clinical Characteristics of Acute Ischemic Stroke Patients
Authors: Anna Gójska-Grymajło
Maciej Zieliński
Dariusz Gąsecki
Kamil Kowalczyk
Mariusz Kwarciany
Barbara Seroczyńska
Walenty M. Nyka
Publication date: 01-09-2018
Publisher: Springer US
Published in: NeuroMolecular Medicine / Issue 3/2018
Print ISSN: 1535-1084
Electronic ISSN: 1559-1174
DOI: https://doi.org/10.1007/s12017-018-8494-x

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

CD271+, CXCR7+, CXCR4+, and CD133+ Stem/Progenitor Cells and Clinical Characteristics of Acute Ischemic Stroke Patients

Abstract

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2018

Piperlongumine Improves Lipopolysaccharide-Induced Amyloidogenesis by Suppressing NF-KappaB Pathway

Potential Role of Brain-Derived Neurotrophic Factor and Dopamine Receptor D2 Gene Variants as Modifiers for the Susceptibility and Clinical Course of Wilson’s Disease

Cerebrospinal Fluid C-Reactive Protein in Parkinson’s Disease: Associations with Motor and Non-motor Symptoms

RNA Sequencing and Pathway Analysis Identify Important Pathways Involved in Hypertrichosis and Intellectual Disability in Patients with Wiedemann–Steiner Syndrome

Effects of Low Phytanic Acid-Concentrated DHA on Activated Microglial Cells: Comparison with a Standard Phytanic Acid-Concentrated DHA

Exercise Training Protects Against Aging-Induced Cognitive Dysfunction via Activation of the Hippocampal PGC-1α/FNDC5/BDNF Pathway