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Cardiovascular Toxicology
Published in: Cardiovascular Toxicology 3/2015

01-07-2015

Protective Effect of Apigenin on Ischemia/Reperfusion Injury of the Isolated Rat Heart

Authors: Jing Hu, Zilin Li, Li-ting Xu, Ai-jun Sun, Xiao-yan Fu, Li Zhang, Lin-lin Jing, An-dong Lu, Yi-fei Dong, Zheng-ping Jia

Published in: Cardiovascular Toxicology | Issue 3/2015

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Abstract

Apigenin (Api), a mainly bioactive component of Apium graveolens L. var. dulce DC. (a traditional Chinese medicinal herb), possesses a wide range of biological activities, including antioxidant effects. It also has been shown to associate with lower prevalence of cardiovascular diseases, but its mechanisms of action remain unclear. The aim of the present study is to investigate the role of Api in isolated rat heart model of ischemia/reperfusion (I/R). Langendorff-perfused isolated rat hearts were used in our study. Api was added to the perfusate before ischemia and during reperfusion in the isolated pulsed rat heart exposed to 30-min ischemia followed by 50-min reperfusion. The treatment with Api conferred a cardioprotective effect, and the treated hearts demonstrated an improved ischemic cardiac functional recovery, a decreased myocardial infarct size, a reduced activities of creatine kinase isoenzyme and lactate dehydrogenase in the coronary flow, a reduced number of apoptotic cardiomyocytes, a reduced activity of caspase-3, up-regulation of the anti-apoptotic protein Bcl-2 and down-regulation of the pro-apoptotic protein Bax. In addition, Api inhibited the phosphorylation of p38 MAPKS during I/R. In conclusion, these observations provide preliminary evidence that Api can protect cardiomyocytes from I-/R-induced injury, at least partially, through the inhibition of p38 MAPKS signaling pathway.
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Metadata
Title
Protective Effect of Apigenin on Ischemia/Reperfusion Injury of the Isolated Rat Heart
Authors
Jing Hu
Zilin Li
Li-ting Xu
Ai-jun Sun
Xiao-yan Fu
Li Zhang
Lin-lin Jing
An-dong Lu
Yi-fei Dong
Zheng-ping Jia
Publication date
01-07-2015
Publisher
Springer US
Published in
Cardiovascular Toxicology / Issue 3/2015
Print ISSN: 1530-7905
Electronic ISSN: 1559-0259
DOI
https://doi.org/10.1007/s12012-014-9290-y

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