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Published in: Cardiovascular Toxicology 2/2015

01-04-2015

Impact of LPS-Induced Cardiomyoblast Cell Apoptosis Inhibited by Earthworm Extracts

Authors: Ping-Chun Li, Yun-Chen Tien, Cecilia Hsuan Day, Peiying Pai, Wei-Wen Kuo, Tung-Sheng Chen, Chia-Hua Kuo, Chang-Hai Tsai, Da-Tong Ju, Chih-Yang Huang

Published in: Cardiovascular Toxicology | Issue 2/2015

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Abstract

Dilong is an earthworm extract with a dense nutritional content, widely used in Chinese herbal medicine to remove stasis and stimulate wound healing. Earthworm extracts are traditionally used by indigenous people throughout the world. How this Dilong inhibits Lipopolysaccharide (LPS)-induced cardiomyoblast cell apoptosis is still unclear. This study investigates the Dilong extract effect on LPS-induced apoptosis in H9c2 cardiomyoblast cells. LPS (1 μg/ml) administration for 24 h induced apoptosis in H9c2 cells. Cell apoptosis was detected using MTT, LDH, TUNEL assay and JC-1 staining. Western blot analysis was used to detect pro-apoptotic and anti-apoptotic proteins. Dilong extract totally blocked the LPS impact, leading to the activation of anti-apoptotic proteins, Bcl-2 and Bcl-xL, stabilized the mitochondria membrane and down-regulated the extrinsic and intrinsic pro-apoptotic proteins, TNF-α, active caspase-8, t-Bid, Bax, active caspase-9 and active caspase-3. Dilong could potentially serve as a cardio protective agent against LPS-induced H9c2 cardiomyoblast cell apoptosis.
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Metadata
Title
Impact of LPS-Induced Cardiomyoblast Cell Apoptosis Inhibited by Earthworm Extracts
Authors
Ping-Chun Li
Yun-Chen Tien
Cecilia Hsuan Day
Peiying Pai
Wei-Wen Kuo
Tung-Sheng Chen
Chia-Hua Kuo
Chang-Hai Tsai
Da-Tong Ju
Chih-Yang Huang
Publication date
01-04-2015
Publisher
Springer US
Published in
Cardiovascular Toxicology / Issue 2/2015
Print ISSN: 1530-7905
Electronic ISSN: 1559-0259
DOI
https://doi.org/10.1007/s12012-014-9281-z

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