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Current HIV/AIDS Reports
Published in: Current HIV/AIDS Reports 1/2022

01-02-2022 | Human Immunodeficiency Virus | Central Nervous System and Cognition (SS Spudich, Section Editor)

Emerging Single-cell Approaches to Understand HIV in the Central Nervous System

Authors: Michael J. Corley, Shelli F. Farhadian

Published in: Current HIV/AIDS Reports | Issue 1/2022

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Abstract

Purpose of Review

This review highlights emerging single-cell sequencing methods relevant to translational studies of HIV in the central nervous system (CNS), summarizes limited single-cell studies of HIV in the CNS, and discusses opportunities for future HIV translational CNS studies.

Recent Findings

Innovative methods utilizing single-cell technologies have advanced the study of genomes, proteomes, transcriptomes, and epigenomes at an enhanced resolution and depth. Single-cell analyses of central nervous system tissue, including autopsy brain and CSF cells, may shed light on CNS perturbations in people living with HIV. New strategies can distinguish distinct molecular identifies of rare infected cells at single-cell level, suggesting an opportunity to uncloak the molecular identity of hidden HIV in the CNS reservoir.

Summary

Adoption of multimodal “omics” analyses to translational HIV studies and tissue compartments beyond blood will be critical to advancing our understanding of viral establishment, persistence, and eradication.
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Metadata
Title
Emerging Single-cell Approaches to Understand HIV in the Central Nervous System
Authors
Michael J. Corley
Shelli F. Farhadian
Publication date
01-02-2022
Publisher
Springer US
Published in
Current HIV/AIDS Reports / Issue 1/2022
Print ISSN: 1548-3568
Electronic ISSN: 1548-3576
DOI
https://doi.org/10.1007/s11904-021-00586-7

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