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Obesity Surgery
Published in: Obesity Surgery 7/2016

01-07-2016 | Original Contributions

Sleeve Gastrectomy Reduces Body Weight and Improves Metabolic Profile also in Obesity-Prone Rats

Authors: Rafael Moncada, Sara Becerril, Amaia Rodríguez, Leire Méndez-Giménez, Beatriz Ramírez, Victoria Catalán, Javier Gómez-Ambrosi, M. Jesús Gil, Secundino Fernández, Javier A. Cienfuegos, Víctor Valentí, Gema Frühbeck

Published in: Obesity Surgery | Issue 7/2016

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Abstract

Background

Susceptibility to obesity is associated with a notable inter-individual variation. The aim of the present study was to compare the effectiveness of sleeve gastrectomy (SG) on weight loss and metabolic profile in obesity-prone (OP) rats vs animals that are non-susceptible to obesity (NSO).

Methods

Young male Wistar rats (n = 101) were put in a diet-induced obesity (DIO) programme with ad libitum access to a high-fed diet (HFD) during 12 months. Body weight and food intake were regularly registered. Thereafter, rats were ranked by final body weight to identify the obesity-prone (OP) (n = 13) and non-susceptible to obesity (NSO) (n = 14) animals. OP and NSO rats were submitted to surgical interventions (sham operation, SG and pair-fed to the amount of food eaten by sleeve-gastrectomized rats). Body weight, food intake, energy expenditure, body temperature, fat pads weight, and metabolic profiling were analysed 4 weeks after surgical or dietary interventions.

Results

SG in both OP and NSO rats decreased body weight as compared to sham and pair-fed groups (P < 0.05), mainly due to reductions in subcutaneous and perirenal fat mass (P < 0.001). Total weight loss achieved in sleeve-gastrectomized OP and NSO rats was higher than that of pair-fed ones (P < 0.05), showing that the SG effect goes beyond caloric restriction. In this regard, sleeve-gastrectomized rats exhibited significantly (P < 0.05) increased basal rectal temperature together with upregulated brown adipose tissue Ucp-1 protein expression levels. A significant (P < 0.05) improvement in insulin sensitivity was also observed in both OP and NSO animals that underwent SG as compared with pair-fed counterparts.

Conclusion

Our findings provide the first evidence that obesity-prone rats also benefit from surgery responding effectively to SG, as evidenced by the significant body weight reduction and the metabolic profile improvement.
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Metadata
Title
Sleeve Gastrectomy Reduces Body Weight and Improves Metabolic Profile also in Obesity-Prone Rats
Authors
Rafael Moncada
Sara Becerril
Amaia Rodríguez
Leire Méndez-Giménez
Beatriz Ramírez
Victoria Catalán
Javier Gómez-Ambrosi
M. Jesús Gil
Secundino Fernández
Javier A. Cienfuegos
Víctor Valentí
Gema Frühbeck
Publication date
01-07-2016
Publisher
Springer US
Published in
Obesity Surgery / Issue 7/2016
Print ISSN: 0960-8923
Electronic ISSN: 1708-0428
DOI
https://doi.org/10.1007/s11695-015-1915-0

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