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Published in: Sport Sciences for Health 2/2018

01-08-2018 | Original Article

The effect of 12 weeks of aerobic exercise on mitochondrial dynamics in cardiac myocytes of type 2 diabetic rats

Authors: Saghi Zafaranieh, Siroos Choobineh, Rahman Soori

Published in: Sport Sciences for Health | Issue 2/2018

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Abstract

Background

Mitochondrial dynamics disorders contribute to the pathogenesis of many diseases involving type 2 diabetes. It appears that exercise training is a strategy for reversing the unbalance between fusion and fission, but, to our understanding, effects of aerobic exercise on this particular issue have not been studied in cardiac myocytes.

Aims

To demonstrate if 12 weeks of aerobic exercise has a significant impact on Dynamin Related Protein 1 (DRP1), Mitofusin2 (MFN2) and Optic Atrophy 1 (OPA1) expressions in cardiac muscle of type 2 diabetic rats.

Methods

10 Wistar rats with an average age of 10 weeks were divided randomly into two groups of five: diabetic control and diabetic exercise. The latter was submitted to a 12-week (5 days a week, 30 min/day) aerobic exercise. 48 h after the last session of exercise, cardiac samples were obtained for genetic experiments. T-test was used for data analysis at p ≤ 0.05.

Results

OPA1 expression was increased significantly (p = 0.03) while MFN2 and DRP1 expressions were elevated insignificantly (p = 0.165, p = 0.19).

Conclusion

This study shows that aerobic training is likely to regulate mitochondrial fission and fusion in cardiac muscle of type 2 diabetic rats. Although more research is necessary.
Footnotes
1
Prolyl-hydroxylases.
 
2
Body weight.
 
3
Streptozotocin.
 
4
Enzyme-linked immunosorbent assay.
 
5
AMP-activated protein kinase.
 
6
Oxidative phosphorylation.
 
Literature
7.
go back to reference Santel A, Fuller MT (2001) Control of mitochondrial morphology by a human mitofusin. J Cell Sci 114:867–874PubMed Santel A, Fuller MT (2001) Control of mitochondrial morphology by a human mitofusin. J Cell Sci 114:867–874PubMed
9.
go back to reference Bach D, Pich S, Soriano FX, Vega N, Baumgartner B, Oriola J, Daugaard JR, Lloberas J, Camps M, Zierath JR, Rabasa-Lhoret R, Wallberg-Henriksson H, Laville M, Palacín M, Vidal H, Rivera F, Brand M, Zorzano A (2003) Mitofusin-2 determines mitochondrial network architecture and mitochondrial metabolism: a novel regulatory mechanism altered in obesity. J Biol Chem 278:17190–17197. https://doi.org/10.1074/jbc.M212754200 CrossRefPubMed Bach D, Pich S, Soriano FX, Vega N, Baumgartner B, Oriola J, Daugaard JR, Lloberas J, Camps M, Zierath JR, Rabasa-Lhoret R, Wallberg-Henriksson H, Laville M, Palacín M, Vidal H, Rivera F, Brand M, Zorzano A (2003) Mitofusin-2 determines mitochondrial network architecture and mitochondrial metabolism: a novel regulatory mechanism altered in obesity. J Biol Chem 278:17190–17197. https://​doi.​org/​10.​1074/​jbc.​M212754200 CrossRefPubMed
11.
go back to reference Kirkwood SP, Munn EA, Brooks GA (1986) Mitochondrial reticulum in limb skeletal muscle. Am J Physiol 251:C395–402CrossRefPubMed Kirkwood SP, Munn EA, Brooks GA (1986) Mitochondrial reticulum in limb skeletal muscle. Am J Physiol 251:C395–402CrossRefPubMed
13.
go back to reference Lee Y, Jeong S, Karbowski M, Smith C, Youle R (2004) Roles of the mammalian mitochondrial fission and fusion mediators Fis1, Drp1, and Opa1 in apoptosis. Mol Biol Cell 15:5001–5015CrossRefPubMedPubMedCentral Lee Y, Jeong S, Karbowski M, Smith C, Youle R (2004) Roles of the mammalian mitochondrial fission and fusion mediators Fis1, Drp1, and Opa1 in apoptosis. Mol Biol Cell 15:5001–5015CrossRefPubMedPubMedCentral
16.
go back to reference Mootha VK, Lindgren CM, Eriksson K-F, Subramanian A, Sihag S, Lehar J, Puigserver P, Carlsson E, Ridderstråle M, Laurila E, Houstis N, Daly MJ, Patterson N, Mesirov JP, Golub TR, Tamayo P, Spiegelman B, Lander ES, Hirschhorn JN, Altshuler D, Groop LC (2003) PGC-1α-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes. Nat Genet 34:267–273. https://doi.org/10.1038/ng1180 CrossRefPubMed Mootha VK, Lindgren CM, Eriksson K-F, Subramanian A, Sihag S, Lehar J, Puigserver P, Carlsson E, Ridderstråle M, Laurila E, Houstis N, Daly MJ, Patterson N, Mesirov JP, Golub TR, Tamayo P, Spiegelman B, Lander ES, Hirschhorn JN, Altshuler D, Groop LC (2003) PGC-1α-responsive genes involved in oxidative phosphorylation are coordinately downregulated in human diabetes. Nat Genet 34:267–273. https://​doi.​org/​10.​1038/​ng1180 CrossRefPubMed
17.
go back to reference Patti ME, Butte AJ, Crunkhorn S, Cusi K, Berria R, Kashyap S, Miyazaki Y, Kohane I, Costello M, Saccone R, Landaker EJ, Goldfine AB, Mun E, DeFronzo R, Finlayson J, Kahn CR, Mandarino LJ (2003) Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes: potential role of PGC1 and NRF1. Proc Natl Acad Sci USA 100:8466–8471. https://doi.org/10.1073/pnas.1032913100 CrossRefPubMed Patti ME, Butte AJ, Crunkhorn S, Cusi K, Berria R, Kashyap S, Miyazaki Y, Kohane I, Costello M, Saccone R, Landaker EJ, Goldfine AB, Mun E, DeFronzo R, Finlayson J, Kahn CR, Mandarino LJ (2003) Coordinated reduction of genes of oxidative metabolism in humans with insulin resistance and diabetes: potential role of PGC1 and NRF1. Proc Natl Acad Sci USA 100:8466–8471. https://​doi.​org/​10.​1073/​pnas.​1032913100 CrossRefPubMed
18.
go back to reference Bach D, Naon D, Pich S, Soriano FX, Vega N, Rieusset J, Laville M, Guillet C, Boirie Y, Wallberg-Henriksson H, Manco M, Calvani M, Castagneto M, Palacín M, Mingrone G, Zierath JR, Vidal H, Zorzano A (2005) Expression of Mfn2, the charcot-marie-tooth neuropathy type 2A gene, in human skeletal muscle. Diabetes 54:2685–2693. https://doi.org/10.2337/diabetes.54.9.2685 CrossRefPubMed Bach D, Naon D, Pich S, Soriano FX, Vega N, Rieusset J, Laville M, Guillet C, Boirie Y, Wallberg-Henriksson H, Manco M, Calvani M, Castagneto M, Palacín M, Mingrone G, Zierath JR, Vidal H, Zorzano A (2005) Expression of Mfn2, the charcot-marie-tooth neuropathy type 2A gene, in human skeletal muscle. Diabetes 54:2685–2693. https://​doi.​org/​10.​2337/​diabetes.​54.​9.​2685 CrossRefPubMed
20.
go back to reference Sebastián D, Hernández-Alvarez MI, Segalés J, Sorianello E, Muñoz JP, Sala D, Waget A, Liesa M, Paz JC, Gopalacharyulu P, Orešič M, Pich S, Burcelin R, Palacín M, Zorzano A (2012) Mitofusin 2 (Mfn2) links mitochondrial and endoplasmic reticulum function with insulin signaling and is essential for normal glucose homeostasis. Proc Natl Acad Sci USA 109:5523–5528. https://doi.org/10.1073/pnas.1108220109 CrossRefPubMed Sebastián D, Hernández-Alvarez MI, Segalés J, Sorianello E, Muñoz JP, Sala D, Waget A, Liesa M, Paz JC, Gopalacharyulu P, Orešič M, Pich S, Burcelin R, Palacín M, Zorzano A (2012) Mitofusin 2 (Mfn2) links mitochondrial and endoplasmic reticulum function with insulin signaling and is essential for normal glucose homeostasis. Proc Natl Acad Sci USA 109:5523–5528. https://​doi.​org/​10.​1073/​pnas.​1108220109 CrossRefPubMed
21.
go back to reference Nochez Y, Arsene S, Gueguen N, Chevrollier A, Ferré M, Guillet V, Desquiret V, Toutain A, Bonneau D, Procaccio V, Amati-Bonneau P, Pisella P-J, Reynier P (2009) Acute and late-onset optic atrophy due to a novel OPA1 mutation leading to a mitochondrial coupling defect. Mol Vis 15:598–608PubMedPubMedCentral Nochez Y, Arsene S, Gueguen N, Chevrollier A, Ferré M, Guillet V, Desquiret V, Toutain A, Bonneau D, Procaccio V, Amati-Bonneau P, Pisella P-J, Reynier P (2009) Acute and late-onset optic atrophy due to a novel OPA1 mutation leading to a mitochondrial coupling defect. Mol Vis 15:598–608PubMedPubMedCentral
30.
go back to reference Mogharnasi M, Gaeini A, Sheikholeslami Vatani D, Rahnama N, Arjmandi B, Bambaeichi E (2011) Effect of acute and prolonged periods of aerobic training and detraining on novel inflammatory marker: the predictive of cardiovascular disease in Wistar rats. Gazz Medica Ital Arch Per Le Sci Mediche 170:307–313 Mogharnasi M, Gaeini A, Sheikholeslami Vatani D, Rahnama N, Arjmandi B, Bambaeichi E (2011) Effect of acute and prolonged periods of aerobic training and detraining on novel inflammatory marker: the predictive of cardiovascular disease in Wistar rats. Gazz Medica Ital Arch Per Le Sci Mediche 170:307–313
32.
go back to reference Taji Tabas A, Mogharnasi M (2015) The effect of 10 week resistance exercise training on serum levels of nesfatin-1 and insulin resistance index in woman with type 2 diabetes. Iran J Diabetes Metab 14:179–188 Taji Tabas A, Mogharnasi M (2015) The effect of 10 week resistance exercise training on serum levels of nesfatin-1 and insulin resistance index in woman with type 2 diabetes. Iran J Diabetes Metab 14:179–188
35.
go back to reference Gaeini AA, Bahramian A, Javidi M (2013) The effect of eight weeks of resistance training on stimulatory and inhibitory factors of cardiac microvascular injuries in Wistar diabetic rats. JME 3:21–32CrossRef Gaeini AA, Bahramian A, Javidi M (2013) The effect of eight weeks of resistance training on stimulatory and inhibitory factors of cardiac microvascular injuries in Wistar diabetic rats. JME 3:21–32CrossRef
36.
go back to reference Jorge L, Paulini J, Silva C, Rampaso R, Luiz R, Lima W, Scavasin G, Schor N (2013) Exercise training improves cardiac mitofusin 2 expression in diabetes. Hypertension 62:613 Jorge L, Paulini J, Silva C, Rampaso R, Luiz R, Lima W, Scavasin G, Schor N (2013) Exercise training improves cardiac mitofusin 2 expression in diabetes. Hypertension 62:613
38.
39.
go back to reference Hernandez-Alvarez MI, Thabit H, Burns N, Shah S, Brema I, Hatunic M, Finucane F, Liesa M, Chiellini C, Naon D, Zorzano A, Nolan JJ (2010) Subjects with early-onset type 2 diabetes show defective activation of the skeletal muscle PGC-1α/mitofusin-2 regulatory pathway in response to physical activity. Diabetes Care 33:645–651. https://doi.org/10.2337/dc09-1305 CrossRefPubMed Hernandez-Alvarez MI, Thabit H, Burns N, Shah S, Brema I, Hatunic M, Finucane F, Liesa M, Chiellini C, Naon D, Zorzano A, Nolan JJ (2010) Subjects with early-onset type 2 diabetes show defective activation of the skeletal muscle PGC-1α/mitofusin-2 regulatory pathway in response to physical activity. Diabetes Care 33:645–651. https://​doi.​org/​10.​2337/​dc09-1305 CrossRefPubMed
40.
go back to reference Holloszy JO, Coyle EF (1984) Adaptations of skeletal muscle to endurance exercise and their metabolic consequences. J Appl Physiol 56:831–838CrossRefPubMed Holloszy JO, Coyle EF (1984) Adaptations of skeletal muscle to endurance exercise and their metabolic consequences. J Appl Physiol 56:831–838CrossRefPubMed
51.
go back to reference Finkel T, Holbrook NJ, Alberti A, Bolognini L, Macciantelli D, Caratelli M, Aviram M, Bełtowski J, Wójcicka G, Marciniak A, Capeillère-Blandin C, Gausson V, Descamps-Latscha B, Witko-Sarsat V, Chen S, Nilsen J, Brinton RD, Droge W, Erel O, Finkel T, Holbrook NJ, Jacobs AH, Winkler A, Castro MG, Lowenstein P, Johnson GL, Lapadat R, Khersonsky O, Tawfik DS, Gu X, Huang Y, Levison BS, Gerstenecker G, DiDonato AJ, Hazen LB, Lee J, Gogonea V, DiDonato JA, Hazen SL, Wu Z, Riwanto M, Gao S, Levison BS, Gu X, Fu X, Wagner MA, Besler C, Gerstenecker G, Zhang R, Li X, DiDonato AJ, Gogonea V, Tang WHW, Smith JD, Plow EF, Fox PL, Shih DM, Lusis AJ, Fisher EA, DiDonato JA, Landmesser U, Hazen SL, Matsuoka Y, Picciano M, La Francois J, Duff K, Mtsuokaa Y, Picciano M, La Francois J, Duff K, Mills E, Dong X, Fudi W, Xu H, Praticò D, Rosenblat M, Gaidukov L, Khersonsky O, Vaya J, Oren R, Tawfik DS, Aviram M, Yin F, Yao J, Sancheti H, Feng T, Melcangi RC, Morgan TE, Finch CE, Pike CJ, Mack WJ, Cadenas E, Brinton RD, McGrath LT, McGleenon BM, Brennan S, McColl D, McILroy S, Passmore AP, Wildsmith KR, Holley M, Savage JC, Skerrett R, Landreth GE, Hayashi H, Gupta A, Iadecola C, Vitali C, Wellington CL, Calabresi L, Cervellati C, Bergamini CM, Mckhann GM, Knopman DS, Chertkow H, Hyman BT, Jack CR, Kawas CH, Klunk WE, Koroshetz WJ, Manly JJ, Mayeux R, Mohs RC, Morris JC, Rossor MN, Scheltens P, Carillo MC, Thies B, Weintraub S, Phelps CH, Gu X, Huang Y, Levison BS, Gerstenecker G, DiDonato AJ, Hazen LB, Lee J, Gogonea V, DiDonato JA, Hazen SL, Klosinski LP, Yao J, Yin F, Fonteh AN, Harrington MG, Christensen TA, Trushina E, Diaz R, Qm C, Simulations MM, Oxon MC, Furlong CE, Richter RJ, Seidel SL, Motulsky AG, Wojtunik-kulesza KA, Oniszczuk A, Oniszczuk T, Waksmundzka-hajnos M (2000) Oxidants, oxidative stress and the biology of ageing. Nature 408:239–247. https://doi.org/10.1038/35041687 CrossRefPubMed Finkel T, Holbrook NJ, Alberti A, Bolognini L, Macciantelli D, Caratelli M, Aviram M, Bełtowski J, Wójcicka G, Marciniak A, Capeillère-Blandin C, Gausson V, Descamps-Latscha B, Witko-Sarsat V, Chen S, Nilsen J, Brinton RD, Droge W, Erel O, Finkel T, Holbrook NJ, Jacobs AH, Winkler A, Castro MG, Lowenstein P, Johnson GL, Lapadat R, Khersonsky O, Tawfik DS, Gu X, Huang Y, Levison BS, Gerstenecker G, DiDonato AJ, Hazen LB, Lee J, Gogonea V, DiDonato JA, Hazen SL, Wu Z, Riwanto M, Gao S, Levison BS, Gu X, Fu X, Wagner MA, Besler C, Gerstenecker G, Zhang R, Li X, DiDonato AJ, Gogonea V, Tang WHW, Smith JD, Plow EF, Fox PL, Shih DM, Lusis AJ, Fisher EA, DiDonato JA, Landmesser U, Hazen SL, Matsuoka Y, Picciano M, La Francois J, Duff K, Mtsuokaa Y, Picciano M, La Francois J, Duff K, Mills E, Dong X, Fudi W, Xu H, Praticò D, Rosenblat M, Gaidukov L, Khersonsky O, Vaya J, Oren R, Tawfik DS, Aviram M, Yin F, Yao J, Sancheti H, Feng T, Melcangi RC, Morgan TE, Finch CE, Pike CJ, Mack WJ, Cadenas E, Brinton RD, McGrath LT, McGleenon BM, Brennan S, McColl D, McILroy S, Passmore AP, Wildsmith KR, Holley M, Savage JC, Skerrett R, Landreth GE, Hayashi H, Gupta A, Iadecola C, Vitali C, Wellington CL, Calabresi L, Cervellati C, Bergamini CM, Mckhann GM, Knopman DS, Chertkow H, Hyman BT, Jack CR, Kawas CH, Klunk WE, Koroshetz WJ, Manly JJ, Mayeux R, Mohs RC, Morris JC, Rossor MN, Scheltens P, Carillo MC, Thies B, Weintraub S, Phelps CH, Gu X, Huang Y, Levison BS, Gerstenecker G, DiDonato AJ, Hazen LB, Lee J, Gogonea V, DiDonato JA, Hazen SL, Klosinski LP, Yao J, Yin F, Fonteh AN, Harrington MG, Christensen TA, Trushina E, Diaz R, Qm C, Simulations MM, Oxon MC, Furlong CE, Richter RJ, Seidel SL, Motulsky AG, Wojtunik-kulesza KA, Oniszczuk A, Oniszczuk T, Waksmundzka-hajnos M (2000) Oxidants, oxidative stress and the biology of ageing. Nature 408:239–247. https://​doi.​org/​10.​1038/​35041687 CrossRefPubMed
Metadata
Title
The effect of 12 weeks of aerobic exercise on mitochondrial dynamics in cardiac myocytes of type 2 diabetic rats
Authors
Saghi Zafaranieh
Siroos Choobineh
Rahman Soori
Publication date
01-08-2018
Publisher
Springer Milan
Published in
Sport Sciences for Health / Issue 2/2018
Print ISSN: 1824-7490
Electronic ISSN: 1825-1234
DOI
https://doi.org/10.1007/s11332-018-0430-9

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