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Molecular Imaging and Biology
Published in: Molecular Imaging and Biology 3/2020

01-06-2020 | Research Article

Simple and Robust Intravital Microscopy Procedures in Hybrid TIE2GFP-BALB/c Transgenic Mice

Authors: Alexandros Marios Sofias, Andreas K. O. Åslund, Nils Hagen, Kristin Grendstad, Sjoerd Hak

Published in: Molecular Imaging and Biology | Issue 3/2020

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Abstract

Purpose

The endeavor of deciphering intricate phenomena within the field of molecular medicine dictates the necessity to investigate tumor/disease microenvironment real-time on cellular level. We, hereby, design simple and robust intravital microscopy strategies, which can be used to elucidate cellular or molecular interactions in a fluorescent mouse model.

Procedures

We crossbred transgenic TIE2GFP mice with nude BALB/c mice, allowing the breeding of immunocompetent and immunodeficient mouse models expressing green fluorescent protein (GFP) in vascular endothelium. Then, we surgically exposed various tissues of interest to perform intravital microscopy.

Results

By utilizing simple tissue preparation procedures and confocal or two-photon microscopy, we produced high-resolution static snapshots, dynamic sequences, and 3D reconstructions of orthotopically grown mammary tumor, skin inflammation, brain, and muscle. The homogenous detection of GFP expressed by endothelial cells and a combination of fluorescence agents enabled landmarking of tumor microenvironment and precise molecular tagging.

Conclusion

Simple intravital microscopy procedures on TIE2GFP mice allowed a real-time multi-color visualization of tissue microenvironment, underlining that robust microscopy strategies are relatively simple and can be readily available for many tissues of interest.
Appendix
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Metadata
Title
Simple and Robust Intravital Microscopy Procedures in Hybrid TIE2GFP-BALB/c Transgenic Mice
Authors
Alexandros Marios Sofias
Andreas K. O. Åslund
Nils Hagen
Kristin Grendstad
Sjoerd Hak
Publication date
01-06-2020
Publisher
Springer International Publishing
Published in
Molecular Imaging and Biology / Issue 3/2020
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-019-01442-2

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