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Molecular Imaging and Biology

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01-06-2020 | Research Article

Paramagnetic Fluorinated Nanoemulsions for in vivo F-19 MRI

Authors: Junsung Rho, Emma Stares, Stephen R. Adams, Deanne Lister, Benjamin Leach, Eric T. Ahrens

Published in: Molecular Imaging and Biology | Issue 3/2020

Abstract

Purpose

We aim to develop perfluorocarbon-based nanoemulsions with improved sensitivity for detection of inflammatory macrophages in situ using F-19 MRI. Towards this goal, we evaluate the feasibility of nanoemulsion formulation incorporating a metal chelate in the fluorous phase which shortens the F-19 longitudinal relaxation rate and image acquisition time.

Procedures

Perfluorinated linear polymers were conjugated to metal-binding tris-diketonate, blended with unconjugated polymers, and emulsified in water. Phospholipid-based surfactant was used to stabilize nanoemulsion and provide biocompatibility. Nanoemulsions were metalated with the addition of ferric salt to the buffer. Physical stability of surfactant and nanoemulsion was evaluated by mass spectrometry and dynamic light scattering measurements. Nanoemulsions were injected intravenously into a murine granuloma inflammation model, and in vivo¹⁹F/¹H MRI at 11.7 T was performed.

Results

We demonstrated stability and biocompatibility of lipid-based paramagnetic nanoemulsions. We investigated potential oxidation of lipid in the presence of metal chelate. As a proof of concept, we performed non-invasive monitoring of macrophage burden in a murine inflammation model following intravenous injection of nanoemulsion using in vivo F-19 MRI.

Conclusion

Lipid-based nanoemulsion probes of perfluorocarbon synthesized with iron-binding fluorinated β-diketones can be formulated for intravenous delivery and inflammation detection in vivo.

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Title: Paramagnetic Fluorinated Nanoemulsions for in vivo F-19 MRI
Authors: Junsung Rho
Emma Stares
Stephen R. Adams
Deanne Lister
Benjamin Leach
Eric T. Ahrens
Publication date: 01-06-2020
Publisher: Springer International Publishing
Published in: Molecular Imaging and Biology / Issue 3/2020
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-019-01415-5

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Paramagnetic Fluorinated Nanoemulsions for in vivo F-19 MRI

Abstract

Purpose

Procedures

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusion

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