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01-06-2021 | Original Article

Oral administration of D-galactose increases brain tricarboxylic acid cycle enzymes activities in Wistar rats

Authors: Josiane Budni, Arleide Braga Brandão, Sabrina da Silva, Michelle Lima Garcez, Francielle Mina, Tatiani Bellettini-Santos, Gabriel Casagrande Zabot, Eduarda Behenck Medeiros, Giselli Scaini, Jade de Oliveira, Emílio Luiz Streck, João Quevedo

Published in: Metabolic Brain Disease | Issue 5/2021

Abstract

D-galactose (D-gal) is a carbohydrate widely distributed in regular diets. However, D-gal administration in rodents is associated with behavioral and neurochemical alterations similar to features observed in aging. In this regard, this study aimed to investigate the effects of D-gal exposure, in different periods, in rats’ brain regions’ activities of creatine kinase (CK) and tricarboxylic acid (TCA) cycle enzymes. Male adult Wistar rats received D-gal (100 mg/kg, gavage) for 1, 2, 4, 6 or 8 weeks. CK and TCA enzymes’ activities were evaluated in rats’ prefrontal cortex and hippocampus. In general, the results showed an increase in citrate synthase (CS) and succinate dehydrogenase (SDH) activities in animals treated with D-gal compared to the control group in the prefrontal cortex and hippocampus. Also, in the fourth week, the malate dehydrogenase (MD) activity increased in the hippocampus of rats that received D-gal compared to control rats. In addition, we observed an increase in the CK activity in the prefrontal cortex and hippocampus in the first and eighth weeks of treatment in the D-gal group compared to the control group. D-gal administration orally administered modulated TCA cycle enzymes and CK activities in the prefrontal cortex and hippocampus, which were also observed in aging and neurodegenerative diseases. However, more studies using experimental models are necessary to understand better the impact and contribution of these brain metabolic abnormalities associated with D-gal consumption for aging.

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Title: Oral administration of D-galactose increases brain tricarboxylic acid cycle enzymes activities in Wistar rats
Authors: Josiane Budni
Arleide Braga Brandão
Sabrina da Silva
Michelle Lima Garcez
Francielle Mina
Tatiani Bellettini-Santos
Gabriel Casagrande Zabot
Eduarda Behenck Medeiros
Giselli Scaini
Jade de Oliveira
Emílio Luiz Streck
João Quevedo
Publication date: 01-06-2021
Publisher: Springer US
Published in: Metabolic Brain Disease / Issue 5/2021
Print ISSN: 0885-7490
Electronic ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-021-00682-y

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Springer Medicine

Oral administration of D-galactose increases brain tricarboxylic acid cycle enzymes activities in Wistar rats

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

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