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Published in: Journal of Mammary Gland Biology and Neoplasia 4/2009

01-12-2009

Methods for Preparing Fluorescent and Neutral Red-Stained Whole Mounts of Mouse Mammary Glands

Authors: John D. Landua, Adriana P. Visbal, Michael T. Lewis

Published in: Journal of Mammary Gland Biology and Neoplasia | Issue 4/2009

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Abstract

Whole mount preparations of mouse mammary glands are useful for evaluating overall changes in growth and morphology, and are essential for detecting and evaluating focal or regionally-localized phenotypes that would be difficult to detect or analyze using other techniques. We present three newly developed methods for preparing whole mounts of mammary glands from genetically-engineered mice expressing fluorescent proteins, as well as using either neutral red or a variety of fluorescent dyes. Unlike traditional hematoxylin- or carmine-stained preparations, neutral red-stained and some fluorescent preparations can be used for several common downstream analyses.
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Metadata
Title
Methods for Preparing Fluorescent and Neutral Red-Stained Whole Mounts of Mouse Mammary Glands
Authors
John D. Landua
Adriana P. Visbal
Michael T. Lewis
Publication date
01-12-2009
Publisher
Springer US
Published in
Journal of Mammary Gland Biology and Neoplasia / Issue 4/2009
Print ISSN: 1083-3021
Electronic ISSN: 1573-7039
DOI
https://doi.org/10.1007/s10911-009-9155-3

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