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Journal of Mammary Gland Biology and Neoplasia

Published in:

01-12-2009

Lentivirus Vectors for Stably Introducing Genes into Mammary Epithelial Cells in Vivo

Authors: Wen Bu, Li Xin, Michael Toneff, Lei Li, Yi Li

Published in: Journal of Mammary Gland Biology and Neoplasia | Issue 4/2009

Abstract

We have reported the use of vectors based on avian leukosis virus (ALV) and ALV envpseudotyped lentivirus for stably introducing genes into somatic mammary cells in vivo for tumor modeling. These vectors require tva transgenic mice for viral infection. Here, we report, and described the method for, stably introducing genes into the mammary gland in mice using intraductal injection of a common lentiviral vector. This method does not need tva-transgenic mice for infection. This in vivo transformation method may be useful for rapid testing of the tumorigenic potential of genes potentially involved in breast carcinogenesis.

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Title: Lentivirus Vectors for Stably Introducing Genes into Mammary Epithelial Cells in Vivo
Authors: Wen Bu
Li Xin
Michael Toneff
Lei Li
Yi Li
Publication date: 01-12-2009
Publisher: Springer US
Published in: Journal of Mammary Gland Biology and Neoplasia / Issue 4/2009
Print ISSN: 1083-3021
Electronic ISSN: 1573-7039
DOI: https://doi.org/10.1007/s10911-009-9154-4

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In Memoriam: H. Allen Tucker (1936–2009)

The RCAS-TVA System for Introduction of Oncogenes into Selected Somatic Mammary Epithelial Cells in Vivo

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