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Published in:

01-11-2010

Association Between Hepatitis B Viral Burden in Chronic Infection and a Functional Single Nucleotide Polymorphism of the PDCD1 Gene

Authors: Linghua Zheng, Dongling Li, Fusheng Wang, Hao Wu, Xiaozhu Li, Junliang Fu, Xinyue Chen, Lizhong Wang, Yang Liu, Shengdian Wang

Published in: Journal of Clinical Immunology | Issue 6/2010

Abstract

Background

PD-1, encoded by PDCD1, is highly expressed on virus-specific T cells and plays critical roles in modulating anti-virus immune responses in chronic viral infection. It is unknown, however, whether polymorphisms of the PDCD1 are associated with viral clearance during chronic viral infections.

Methodology and principal findings

Here, we used the polymerase chain reaction-restriction fragment length polymorphism method to genotype two single nucleotide polymorphisms (SNPs) of PDCD1 in 502 patients with chronic hepatitis B virus (HBV) infection and 359 healthy controls to determine the association between PDCD1 genotypes and serum viral load as well as the risk of chronic infection. Our results showed that although neither the P7209^C/T SNP site nor the P8737^A/G site was associated with the risk of chronic HBV infection, the P7209 ^T allele in intron 4 is significantly associated with lower viral burden in the blood. Using a luciferase reporter assay, we demonstrated that the P7209 ^T allele creates a negative cis-element for gene transcription.

Conclusions and significance

Our data provide the first evidence that PDCD1 polymorphisms is a genetic factor in pathogenesis of chronic viral infection and reveal the functional significance of the P7209 SNP of the PDCD1.

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Title: Association Between Hepatitis B Viral Burden in Chronic Infection and a Functional Single Nucleotide Polymorphism of the PDCD1 Gene
Authors: Linghua Zheng
Dongling Li
Fusheng Wang
Hao Wu
Xiaozhu Li
Junliang Fu
Xinyue Chen
Lizhong Wang
Yang Liu
Shengdian Wang
Publication date: 01-11-2010
Publisher: Springer US
Published in: Journal of Clinical Immunology / Issue 6/2010
Print ISSN: 0271-9142
Electronic ISSN: 1573-2592
DOI: https://doi.org/10.1007/s10875-010-9450-1

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Association Between Hepatitis B Viral Burden in Chronic Infection and a Functional Single Nucleotide Polymorphism of the PDCD1 Gene

Abstract

Background

Methodology and principal findings

Conclusions and significance

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Abstract

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Conclusions and significance

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