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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 9/2017

01-09-2017 | Embryo Biology

Morphokinetic parameters from a time-lapse monitoring system cannot accurately predict the ploidy of embryos

Authors: Jingye Zhang, Wenrong Tao, Hui Liu, Guanling Yu, Mei Li, Shuiying Ma, Keliang Wu

Published in: Journal of Assisted Reproduction and Genetics | Issue 9/2017

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Abstract

Purpose

This study aimed to test whether there is an association between embryo morphokinetic parameters and ploidy status.

Methods

Patients with high risk of aneuploidy were analyzed by time-lapse microscopy combined with preimplantation genetic screening (PGS). Accordingly, 256 blastocysts from 75 patients were subjected to trophectoderm biopsy and microarray comparative genomic hybridization (array-CGH). Blastocyst development process was analyzed using time-lapse images.

Results

Morphokinetic parameters: tPNf, t2, t3, t4, t5, t8, t9, tcom, tM, tSB, tB, tEB, CC1, CC2, CC3, S2, S3, t5-t2, and tB-tSB showed no significant difference in euploid embryos compared to aneuploid counterparts. In addition, two risk models based on previously published morphokinetic parameters failed to segregate euploid from aneuploid embryos.

Conclusions

Morphokinetic parameters subjected to investigation in the present study failed to improve the chance of selecting euploid embryos.
Literature
1.
Fragouli E, Wells D. Aneuploidy in the human blastocyst. Cytogenet Genome Res. 2011;133(2–4):149–59.CrossRefPubMed
2.
Angell RR, Aitken RJ, van Look PF, et al. Chromosome abnormalities in human embryos after in vitro fertilization. Nature. 1983;303(5915):336–8.CrossRefPubMed
3.
4.
Demko ZP, Simon AL, McCoy RC, et al. Effects of maternal age on euploidy rates in a large cohort of embryos analyzed with 24-chromosome single-nucleotide polymorphism-based preimplantation genetic screening. Fertil Steril. 2016;105(5):1307–13.CrossRefPubMed
5.
Scott RT Jr, Upham KM, Forman EJ, et al. Blastocyst biopsy with comprehensive chromosome screening and fresh embryo transfer significantly increases in vitro fertilization implantation and delivery rates: a randomized controlled trial. Fertil Steril. 2013;100(3):697–703.CrossRefPubMed
6.
Chang J, Boulet SL, Jeng G, et al. Outcomes of in vitro fertilization with preimplantation genetic diagnosis: an analysis of the United States Assisted Reproductive Technology Surveillance Data, 2011-2012. Fertil Steril. 2016;105(2):394–400.CrossRefPubMed
7.
8.
Hellani A, Abu-Amero K, Azouri J, et al. Successful pregnancies after application of array-comparative genomic hybridization in PGS-aneuploidy screening. Reprod BioMed Online. 2008;17(6):841–7.CrossRefPubMed
9.
Johnson DS, Gemelos G, Baner J, et al. Preclinical validation of a microarray method for full molecular karyotyping of blastomeres in a 24-h protocol. Hum Reprod. 2010;25(4):1066–75.CrossRefPubMedPubMedCentral
10.
Treff NR, Tao X, Ferry KM, et al. Development and validation of an accurate quantitative real-time polymerase chain reaction-based assay for human blastocyst comprehensive chromosomal aneuploidy screening. Fertil Steril. 2012;97(4):819–24.CrossRefPubMed
11.
Tan Y, Yin X, Zhang S, et al. Clinical outcome of preimplantation genetic diagnosis and screening using next generation sequencing. Gigascience. 2014;3(1):30.CrossRefPubMedPubMedCentral
12.
Bielanska M, Tan SL, Ao A. High rate of mixoploidy among human blastocysts cultured in vitro. Fertil Steril. 2002;78(6):1248–53.CrossRefPubMed
13.
Esfandiari N, Bunnell ME, Casper RF. Human embryo mosaicism: did we drop the ball on chromosomal testing? J Assist Reprod Genet. 2016;33(11):1439–44.CrossRefPubMed
14.
Kirkegaard K, Kesmodel US, Hindkjaer JJ, et al. Time-lapse parameters as predictors of blastocyst development and pregnancy outcome in embryos from good prognosis patients: a prospective cohort study. Hum Reprod. 2013;28(10):2643–51.CrossRefPubMed
15.
Meseguer M, Herrero J, Tejera A, et al. The use of morphokinetics as a predictor of embryo implantation. Hum Reprod. 2011;26(10):2658–71.CrossRefPubMed
16.
Desai N, Ploskonka S, Goodman LR, et al. Analysis of embryo morphokinetics, multinucleation and cleavage anomalies using continuous time-lapse monitoring in blastocyst transfer cycles. Reprod Biol Endocrinol. 2014;12:54.CrossRefPubMedPubMedCentral
17.
Chawla M, Fakih M, Shunnar A, et al. Morphokinetic analysis of cleavage stage embryos and its relationship to aneuploidy in a retrospective time-lapse imaging study. J Assist Reprod Genet. 2015;32(1):69–75.CrossRefPubMed
18.
Basile N, Nogales Mdel C, Bronet F, et al. Increasing the probability of selecting chromosomally normal embryos by time-lapse morphokinetics analysis. Fertil Steril. 2014;101(3):699–704.CrossRefPubMed
19.
Campbell A, Fishel S, Bowman N, et al. Modelling a risk classification of aneuploidy in human embryos using non-invasive morphokinetics. Reprod BioMed Online. 2013;26(5):477–85.CrossRefPubMed
20.
Campbell A, Fishel S, Bowman N, et al. Retrospective analysis of outcomes after IVF using an aneuploidy risk model derived from time-lapse imaging without PGS. Reprod BioMed Online. 2013;27(2):140–6.CrossRefPubMed
21.
Rienzi L, Capalbo A, Stoppa M, et al. No evidence of association between blastocyst aneuploidy and morphokinetic assessment in a selected population of poor-prognosis patients: a longitudinal cohort study. Reprod BioMed Online. 2015;30(1):57–66.CrossRefPubMed
22.
Kramer YG, Kofinas JD, Melzer K, et al. Assessing morphokinetic parameters via time lapse microscopy (TLM) to predict euploidy: are aneuploidy risk classification models universal? J Assist Reprod Genet. 2014;31(9):1231–42.CrossRefPubMedPubMedCentral
23.
24.
Del Carmen Nogales M, Bronet F, Basile N, et al. Type of chromosome abnormality affects embryo morphology dynamics. Fertil Steril. 2017;107(1):229–35.CrossRefPubMed
25.
Minasi MG, Colasante A, Riccio T, et al. Correlation between aneuploidy, standard morphology evaluation and morphokinetic development in 1730 biopsied blastocysts: a consecutive case series study. Hum Reprod. 2016;31(10):2245–54.CrossRefPubMed
26.
Gardner DK, Lane M. Culture and selection of viable blastocysts: a feasible proposition for human IVF? Hum Reprod Update. 1997;3(4):367–82.CrossRefPubMed
27.
Ciray HN, Aksoy T, Goktas C, et al. Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study. J Assist Reprod Genet. 2012;29(9):891–900.CrossRefPubMedPubMedCentral
28.
Reed ML, Hamic A, Thompson DJ, et al. Continuous uninterrupted single medium culture without medium renewal versus sequential media culture: a sibling embryo study. Fertil Steril. 2009;92(5):1783–6.CrossRefPubMed
29.
Morbeck DE, Baumann NA, Oglesbee D. Composition of single-step media used for human embryo culture. Fertil Steril. 2017;107(4):1055–60.CrossRefPubMed
30.
Liu Y, Chapple V, Roberts P, et al. Prevalence, consequence, and significance of reverse cleavage by human embryos viewed with the use of the embryoscope time-lapse video system. Fertil Steril. 2014;102(5):1295–300.CrossRefPubMed
31.
Rubio I, Kuhlmann R, Agerholm I, et al. Limited implantation success of direct-cleaved human zygotes: a time-lapse study. Fertil Steril. 2012;98(6):1458–63.CrossRefPubMed
32.
Ambroggio J, Gindoff PR, Dayal MB, et al. Multinucleation of a sibling blastomere on day 2 suggests unsuitability for embryo transfer in IVF-preimplantation genetic screening cycles. Fertil Steril. 2011;96(4):856–9.CrossRefPubMed
33.
Kligman I, Benadiva C, Alikani M, et al. The presence of multinucleated blastomeres in human embryos is correlated with chromosomal abnormalities. Hum Reprod. 1996;11(7):1492–8.CrossRefPubMed
Metadata
Title
Morphokinetic parameters from a time-lapse monitoring system cannot accurately predict the ploidy of embryos
Authors
Jingye Zhang
Wenrong Tao
Hui Liu
Guanling Yu
Mei Li
Shuiying Ma
Keliang Wu
Publication date
01-09-2017
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 9/2017
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-017-0965-8

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