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Journal of Assisted Reproduction and Genetics

Published in:

01-09-2012 | ASSISTED REPRODUCTION TECHNOLOGIES

Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study

Authors: Haydar Nadir Ciray, Turan Aksoy, Cihan Goktas, Bilgen Ozturk, Mustafa Bahceci

Published in: Journal of Assisted Reproduction and Genetics | Issue 9/2012

Abstract

Objective

To compare the dynamics of early development between embryos cultured in single and sequential media.

Design

Randomized, comparative study.

Setting

Private IVF centre.

Patients

A total of 446 metaphase II oocytes from 51 couples who underwent oocyte retrieval procedure for intracytoplasmic sperm injection. Forty-nine resulted in embryo transfer.

Intervention

Oocytes were split between single and sequential media produced by the same manufacturer and cultured in a time-lapse incubator.

Main outcome measures

Morphokinetic parameters until the embryos reached the 5-cell stage (t5), utilization, clinical pregnancy and implantation rates.

Results

Embryos cultured in single media were advanced from the first mitosis cycle and reached 2- to 5-cell stages earlier. There was not any difference between the durations for cell cycle two (cc2 = t3-t2) and s2 (t4-t3). The utilization, clinical pregnancy and implantation rates did not differ between groups. The proportion of cryopreserved day6 embryos to two pronuclei oocytes was significantly higher in sequential than in single media.

Conclusions

Morphokinetics of embryo development vary between single and sequential culture media at least until the 5-cell stage. The overall clinical and embryological parameters remain similar regardless of the culture system.

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Title: Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study
Authors: Haydar Nadir Ciray
Turan Aksoy
Cihan Goktas
Bilgen Ozturk
Mustafa Bahceci
Publication date: 01-09-2012
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 9/2012
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-012-9818-7

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study

Abstract

Objective

Design

Setting

Patients

Intervention

Main outcome measures

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objective

Design

Setting

Patients

Intervention

Main outcome measures

Results

Conclusions

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