Top

Journal of Assisted Reproduction and Genetics

Published in:

01-06-2009 | ASSISTED REPRODUCTION

Follicle curetting at the time of oocyte retrieval increases the oocyte yield

Authors: Stephanie K. Dahl, Sara Cannon, Mira Aubuchon, Daniel B. Williams, Jared C. Robins, Michael A. Thomas

Published in: Journal of Assisted Reproduction and Genetics | Issue 6/2009

Abstract

Purpose

To determine whether follicle curetting at the time of oocyte retrieval increases oocyte yield.

Methods

Retrospective review of all patients who underwent oocyte retrieval from July 1, 2003 to June 30, 2005.

Main outcome measure

Number of oocytes retrieved. Secondary outcome measures: retrieval time, number of cryopreserved embryos, pregnancy rates, and incidence of ovarian hyperstimulation syndrome.

Results

There were no differences in patient demographics, antral follicle count, cycle stimulation characteristics, fertilization rates, embryo quantity or quality, embryo cryopreservation rates, clinical pregnancy rates, live birth rates, or ovarian hyperstimulation syndrome between the groups. Retrievals that utilized curetting took three minutes longer. Follicle curetting significantly increased the number of oocytes retrieved, 13.9 ± 0.6 compared to 11.4 ± 0.6 oocytes without curetting (P = 0.003). The quantity of mature oocytes was also increased with curetting (10.3 ± 0.5 versus 8.4 ± 0.5, P = 0.006).

Conclusions

This study demonstrated that follicle curetting significantly increased oocyte yield. While it did not increase live birth rates, this increase in oocyte yield should lead to increased numbers of embryos for selection at transfer and increased embryos for cryopreservation.

Yao MWM, Schust D. Infertility. In: Berek JS, editor. Novak’s Gynecology. 13th ed. Philadelphia PA: Lippincott; 2002. p. 1029.

Waterstone JJ, Parsons JH. A prospective study to investigate the value of flushing follicles during transvaginal ultrasound-directed follicle aspiration. Fertil Steril. 1992;57(1):221–3.PubMed

Tan SL, Waterstone J, Wren M, Parsons J. A prospective randomized study comparing aspiration only with aspiration and flushing for transvaginal ultrasound-directed oocyte recovery. Fertil Steril. 1992;58(2):356–60.PubMed

Kingsland CR, Taylor CT, Aziz N, Bickerton N. Is follicular flushing necessary for oocyte retrieval? A randomized trial. Hum Reprod. 1991;6(3):382–3.PubMed

Knight DC, Tyler JP, Driscoll GL. Follicular flushing at oocyte retrieval: a reappraisal. Aust N Z J Obstet Gynaecol. 2001;41(2):210–3. doi:10.1111/j.1479-828X.2001.tb01212.x.PubMedCrossRef

Scott RT, Hofmann GE, Muasher SJ, Acosta AA, Kreiner DK, Rosenwaks Z. A prospective randomized comparison of single- and double-lumen needles for transvaginal follicular aspiration. J In Vitro Fert Embryo Transf. 1989;6(2):98–100. doi:10.1007/BF01130734.PubMedCrossRef

Aziz N, Biljan MM, Taylor CT, Manasse PR, Kingsland CR. Effect of aspirating needle calibre on outcome of in-vitro fertilization. Hum Reprod. 1993;8(7):1098–100.PubMed

Awonuga A, Waterstone J, Oyesanya O, Curson R, Nargund G, Parsons J. A prospective randomized study comparing needles of different diameters for transvaginal ultrasound-directed follicle aspiration. Fertil Steril. 1996;65(1):109–13.PubMed

The Practice Committee of the American Society for Reproductive Medicine. Ovarian Hyperstimulation Syndrome. Fertil Steril. 2003;80(5):1309–14. doi:10.1016/S0015-0282(03)02194-0.CrossRef

10.

Hendriks DJ, Kwee J, Mol BW, Te Velde ER, Broekmans FJ. Ultrasonography as a tool for the prediction of outcome in IVF patients: a comparative meta-analysis of ovarian volume and antral follicle count. Fertil Steril. 2007;87(4):764–75. doi:10.1016/j.fertnstert.2006.11.006.PubMedCrossRef

11.

Scott RT, Hofmann GE, Muasher SJ, Acosta AA, Kreiner DK, Rosenwaks Z. Correlation of follicular diameter with oocyte recovery and maturity at the time of transvaginal follicular aspiration. J In Vitro Fert Embryo Transf. 1989;6(2):73–5. doi:10.1007/BF01130729.PubMedCrossRef

12.

Chung K, Coutifaris C, Chalian R, Lin K, Ratcliffee SJ, Castelbaum AJ, et al. Factors influencing adverse perinatal outcomes through use of in vitro fertilization. Fertil Steril. 2006;86(6):1634–41. doi:10.1016/j.fertnstert.2006.04.038.PubMedCrossRef

13.

Kosaka K, Fujiwara H, Yoshioka S, Fujii S. Vascular endothelial growth factor production by circulating immune cells is elevated in ovarian hyperstimulation syndrome. Hum Reprod. 2007;22(6):1647–51. doi:10.1093/humrep/dem042.PubMedCrossRef

14.

Ludwig AK, Glawatz M, Griesinger G, Diedrich K, Ludwig M. Perioperative and post-operative complications of transvaginal ultrasound-guided oocyte retrieval: prospective study of > 1000 oocyte retrievals. Hum Reprod. 2006;21(12):3235–40. doi:10.1093/humrep/del278.PubMedCrossRef

15.

Papanikolaou EG, Pozzobon C, Kolibianakis EM, Camus M, Tournaye H, Fatemi HM, et al. Incidence and prediction of ovarian hyperstimulation syndrome in women undergoing gonadotropin-releasing hormone antagonist in vitro fertilization cycles. Fertil Steril. 2006;85(1):112–20. doi:10.1016/j.fertnstert.2005.07.1292.PubMedCrossRef

Title: Follicle curetting at the time of oocyte retrieval increases the oocyte yield
Authors: Stephanie K. Dahl
Sara Cannon
Mira Aubuchon
Daniel B. Williams
Jared C. Robins
Michael A. Thomas
Publication date: 01-06-2009
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 6/2009
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-009-9323-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Follicle curetting at the time of oocyte retrieval increases the oocyte yield

Abstract

Purpose

Methods

Main outcome measure

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Main outcome measure

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 6/2009

Improvement of in vitro oocyte maturation with lectin supplementation and expression analysis of Cx43, GDF-9, FGF-4 and Fibronectin mRNA transcripts in Buffalo (Bubalus bubalis)

Vitrification versus slow freezing gives excellent survival, post warming embryo morphology and pregnancy outcomes for human cleaved embryos

Comment on Winke T et al. J Assist Reprod Genet DOI 10.1007/S10815-008-9277-3

Prediction of high ovarian response to controlled ovarian hyperstimulation: anti-Müllerian hormone versus small antral follicle count (2–6 mm)

Antioxidant supplementation of culture medium during embryo development and/or after vitrification-warming; which is the most important?

Application of intra- and extracellular sugars and dimethylsulfoxide to human oocyte cryopreservation