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01-10-2014

The Effect of Taurine on the Relationship Between NO, ADMA and Homocysteine in Endotoxin-Mediated Inflammation in HUVEC Cultures

Authors: Ozge Tugce Pasaoglu, Nurten Turkozkan, Mustafa Ark, Belgin Polat, Mehmet Agilli, Halil Yaman

Published in: Inflammation | Issue 5/2014

Abstract

The aim of our study was to investigate the effect of taurine on the relationship between nitric oxide (NO), asymmetric dimethylarginine (ADMA) and homocysteine (Hcy) in endotoxin-induced human umblical vein endothelial cell (HUVEC) cultures. For this reason, four groups were formed (n = 12). Control group consists of HUVEC cultures without any treatment. Lipopolysaccharide (LPS) and LPS + taurine groups were treated with 10 μg/mL endotoxin, 5 μg/mL taurine and endotoxin + taurine (same doses), respectively. Nitrite/nitrate (NOx), ADMA and Hcy levels were measured. There was a significant increase of NOx, ADMA and Hcy in endotoxemia (p < 0.05). Taurine treatment elevated NOx levels significantly (p < 0.01) in taurine and LPS + taurine group compared to control group, while it reduced NOx levels compared to LPS group. In contrast, taurine decreased ADMA levels to the control level both in taurine and taurine + LPS group compared to LPS. Hcy levels increased significantly compared to taurine group (p < 0.05) and did not change compared to LPS group. Taurine was effective on ADMA–NO relationship whereas no beneficial effect was observed in Hcy levels (p < 0.05).

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Title: The Effect of Taurine on the Relationship Between NO, ADMA and Homocysteine in Endotoxin-Mediated Inflammation in HUVEC Cultures
Authors: Ozge Tugce Pasaoglu
Nurten Turkozkan
Mustafa Ark
Belgin Polat
Mehmet Agilli
Halil Yaman
Publication date: 01-10-2014
Publisher: Springer US
Published in: Inflammation / Issue 5/2014
Print ISSN: 0360-3997
Electronic ISSN: 1573-2576
DOI: https://doi.org/10.1007/s10753-014-9868-3

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