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01-12-2006 | Original Paper

Transepithelial Leak in Barrett’s Esophagus

Authors: J. M. Mullin, M. C. Valenzano, S. Trembeth, P. D. Allegretti, J. J. Verrecchio, J. D. Schmidt, V. Jain, J. B. Meddings, G. Mercogliano, J. J. Thornton

Published in: Digestive Diseases and Sciences | Issue 12/2006

Abstract

Using orally administered sucrose as a probe of gastrointestinal permeability, this study focused on determining whether Barrett’s metaplasia exhibits a paracellular transepithelial leak to small nonelectrolytes. Subjects in five separate classes (nonendoscoped, asymptomatic controls; endoscoped, asymptomatic controls; gastroesophageal reflux disease without mucosal complications; grossly visible esophagitis; and Barrett’s esophagus) consumed a sucrose solution at bedtime and collected all overnight urine. Urine volume was measured and sucrose concentration was determined by high-performance liquid chromatography. Patients with Barrett’s were observed to exhibit a transepithelial leak to sucrose whose mean value was threefold greater than that seen in healthy control subjects or patients with reflux but without any mucosal defect. A parallel study of claudin tight junction proteins in endoscopy biopsy samples showed that whereas Barrett’s metaplasia contains dramatically more claudin-2 and claudin-3 than is found in normal esophageal mucosa, it is markedly lower in claudins 1 and 5, indicating very different tight junction barriers.

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Title: Transepithelial Leak in Barrett’s Esophagus
Authors: J. M. Mullin
M. C. Valenzano
S. Trembeth
P. D. Allegretti
J. J. Verrecchio
J. D. Schmidt
V. Jain
J. B. Meddings
G. Mercogliano
J. J. Thornton
Publication date: 01-12-2006
Publisher: Kluwer Academic Publishers-Plenum Publishers
Published in: Digestive Diseases and Sciences / Issue 12/2006
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-006-9478-5

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