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Published in: Breast Cancer Research and Treatment 2/2012

01-04-2012 | Preclinical Study

Bone metastasis in a novel breast cancer mouse model containing human breast and human bone

Authors: Tian-Song Xia, Guo-Zhu Wang, Qiang Ding, Xiao-An Liu, Wen-Bin Zhou, Yi-Fen Zhang, Xiao-Ming Zha, Qing Du, Xiao-Jian Ni, Jue Wang, Su-Yu Miao, Shui Wang

Published in: Breast Cancer Research and Treatment | Issue 2/2012

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Abstract

In practice, investigations for bone metastasis of breast cancer rely heavily on models in vivo. Lacking of such ideal model makes it difficult to study the whole process or accurate mechanism of each step of this metastatic disease. Development of xenograft mouse models has made great contributions in this area. Currently, the best animal model of breast cancer metastasizing to bone is NOD/SCID-hu models containing human bone, which makes it possible to let the breast cancer cells and the bone target of osteotropic metastasis be both of human origin. We have developed a novel mouse model containing both human bone and breast, and proved it functional and reliable. In this study, a set of human breast cancer cell line including MDA-MB-231, MDA-MB-231BO, MCF-7, ZR-75-1 and SUM1315 were characterized their osteotropism in this model. A specific cell line SUM1315 made species-specific bone metastasis, certifying the osteotropism-identification utility of the novel mouse model. Furthermore, gene expression and microRNA expression profiling analysis were done to the two SUM1315 derived sub lines isolated and purified from the orthotopic and metastatic xenograft. In addition, to demonstrate the disparity between the “spontaneous” and “forced” bone metastasis in mouse model, MDA-MB-231 cells were inoculated into both the human implants in this model simultaneously, and then primary cultured and profiling analyzed. Supported by overall results of profiling analyses, this study suggested the novel model was a useful tool for understanding, preventing and treating bone metastasis of breast cancer, meanwhile it had provided significant information for further investigations.
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Metadata
Title
Bone metastasis in a novel breast cancer mouse model containing human breast and human bone
Authors
Tian-Song Xia
Guo-Zhu Wang
Qiang Ding
Xiao-An Liu
Wen-Bin Zhou
Yi-Fen Zhang
Xiao-Ming Zha
Qing Du
Xiao-Jian Ni
Jue Wang
Su-Yu Miao
Shui Wang
Publication date
01-04-2012
Publisher
Springer US
Published in
Breast Cancer Research and Treatment / Issue 2/2012
Print ISSN: 0167-6806
Electronic ISSN: 1573-7217
DOI
https://doi.org/10.1007/s10549-011-1496-0

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