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Breast Cancer Research and Treatment
Published in: Breast Cancer Research and Treatment 2/2007

01-10-2007 | Preclinical Study

Mesenchymal stem cells as a vehicle for targeted delivery of CRAds to lung metastases of breast carcinoma

Authors: Mariam A. Stoff-Khalili, Angel A. Rivera, J. Michael Mathis, N. Sanjib Banerjee, Amanda S. Moon, A. Hess, Rodney P. Rocconi, T. Michael Numnum, M. Everts, Louise T. Chow, Joanne T. Douglas, Gene P. Siegal, Zeng B. Zhu, Hans Georg Bender, Peter Dall, Alexander Stoff, Larissa Pereboeva, David T. Curiel

Published in: Breast Cancer Research and Treatment | Issue 2/2007

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Abstract

Purpose

Alternative and complementary therapeutic strategies need to be developed for metastatic breast cancer. Virotherapy is a novel therapeutic approach for the treatment of cancer in which the replicating virus itself is the anticancer agent. However, the success of virotherapy has been limited due to inefficient virus delivery to the tumor site. The present study addresses the utility of human mesenchymal stem cells (hMSCs) as intermediate carriers for conditionally replicating adenoviruses (CRAds) to target metastatic breast cancer in vivo.

Experimental design

HMSC were transduced with CRAds. We used a SCID mouse xenograft model to examine the effects of systemically injected CRAd loaded hMSC or CRAd alone on the growth of MDA-MB-231 derived pulmonary metastases (experimental metastases model) in vivo and on overall survival.

Results

Intravenous injection of CRAd loaded hMSCs into mice with established MDA-MB-231 pulmonary metastatic disease homed to the tumor site and led to extended mouse survival compared to mice treated with CRAd alone.

Conclusion

Injected hMSCs transduced with CRAds suppressed the growth of pulmonary metastases, presumably through viral amplification in the hMSCs. Thus, hMSCs may be an effective platform for the targeted delivery of CRAds to distant cancer sites such as metastatic breast cancer.
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Metadata
Title
Mesenchymal stem cells as a vehicle for targeted delivery of CRAds to lung metastases of breast carcinoma
Authors
Mariam A. Stoff-Khalili
Angel A. Rivera
J. Michael Mathis
N. Sanjib Banerjee
Amanda S. Moon
A. Hess
Rodney P. Rocconi
T. Michael Numnum
M. Everts
Louise T. Chow
Joanne T. Douglas
Gene P. Siegal
Zeng B. Zhu
Hans Georg Bender
Peter Dall
Alexander Stoff
Larissa Pereboeva
David T. Curiel
Publication date
01-10-2007
Publisher
Springer US
Published in
Breast Cancer Research and Treatment / Issue 2/2007
Print ISSN: 0167-6806
Electronic ISSN: 1573-7217
DOI
https://doi.org/10.1007/s10549-006-9449-8

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