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16-01-2022 | Candida Balanitis | Original Article

Inhibitory effect of 405-nm blue LED light on the growth of Candida albicans and Streptococcus mutans dual-species biofilms on denture base resin

Authors: Chiaki Tsutsumi-Arai, Yuki Arai, Chika Terada-Ito, Takahiro Imamura, Seiko Tatehara, Shinji Ide, Jumpei Shirakawa, Noriyuki Wakabayashi, Kazuhito Satomura

Published in: Lasers in Medical Science | Issue 4/2022

Abstract

We investigated whether irradiation with 405-nm blue LED light could inhibit the growth of not only single- but dual-species biofilms formed by Candida albicans and Streptococcus mutans on denture base resin and cause the alteration in gene expression related to adhesion and biofilm formation. C. albicans and S. mutans single-/dual-species biofilms were formed on the denture base specimens. The biofilms were irradiated with 405-nm blue LED light (power density output: 280 mW/cm²) for 0 (control) and 40 min. Dual-species biofilms were analyzed using CFU assay and fluorescence microscopy, and single-/dual-species biofilms were analyzed using alamarBlue assays and gene expression analysis. To assess the inhibitory effect of irradiation on dual-species biofilms, specimens after irradiation were aerobically incubated for 12 h. After incubation, the inhibition of growth was assessed using CFU assays and fluorescence microscopy. Data were analyzed using the Mann–Whitney U or Student’s t test (p < 0.05). Irradiation produced a significant inhibitory effect on biofilms. Fluorescence microscopy revealed that almost all C. albicans and S. mutans cells were killed by irradiation, and there was no notable difference in biofilm thickness immediately after irradiation and after irradiation and incubation for 12 h. alamarBlue assays indicated the growth of the biofilms was inhibited for 12–13 h. The expression of genes associated with adhesion and biofilm formation—als1 in C. albicans and ftf, gtfC, and gtfB in S. mutans—significantly reduced by irradiation. Irradiation with 405-nm blue LED light effectively inhibited the growth of C. albicans and S. mutans dual-species biofilms for 12 h.

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Title: Inhibitory effect of 405-nm blue LED light on the growth of Candida albicans and Streptococcus mutans dual-species biofilms on denture base resin
Authors: Chiaki Tsutsumi-Arai
Yuki Arai
Chika Terada-Ito
Takahiro Imamura
Seiko Tatehara
Shinji Ide
Jumpei Shirakawa
Noriyuki Wakabayashi
Kazuhito Satomura
Publication date: 16-01-2022
Publisher: Springer London
Keywords: Candida Balanitis
Candida Albicans
Denture
Streptococci
Published in: Lasers in Medical Science / Issue 4/2022
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-022-03507-1

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Inhibitory effect of 405-nm blue LED light on the growth of Candida albicans and Streptococcus mutans dual-species biofilms on denture base resin

Abstract

Keynote webinar | Spotlight on sleep in brain health

Springer Medicine

Abstract

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