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Lasers in Medical Science
Published in: Lasers in Medical Science 4/2022

13-01-2022 | Laser | Original Article

In vivo efficacy of low-level laser therapy on bone regeneration

Authors: Birtan Tolga Yılmaz, Abdullah C. Akman, Alper Çetinkaya, Can Colak, Benay Yıldırım, Özlem Özer Yücel, Güliz N. Güncü, Rahime M. Nohutcu

Published in: Lasers in Medical Science | Issue 4/2022

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Abstract

Purpose

In clinical use of low-level laser therapy for bone regeneration (LLLT), application protocol (dose, duration, and repetitions) has not been established. This study aimed to depict a reliable dosage of LLLT by evaluating the efficacy of different dosing of LLLT (diode) on the healing of rabbit cranial defects.

Methods

Critical size defects were prepared in calvarias of 26 New Zealand White Rabbits in such each animal containing both test and control groups. Test groups were irradiated with 4 Joule/cm2 (j/cm2), 6 j/cm2, and 8 j/cm2. The rabbits were subjected to six times of laser treatments in 10 days. At the end of the second week, 5 rabbits were sacrificed for histopathological and immunohistochemical analyses. At the 4th and 8th weeks, 20 rabbits (10 each) were sacrificed for micro-CT and histopathological analyses.

Results

Micro-CT evaluation revealed improved new bone formation in all test groups compared to the control group. 6 j/cm2 group demonstrated the highest bone formation. The highest bone morphogenic protein -2 levels were found in the 4 j/cm2 group. Osteocalcin expression was significantly higher in 4 j/cm2 group.

Conclusions

Our findings indicate that LLLT have a positive effect on new bone formation. The high efficacy of doses of 4 j/cm2 and 6 j/cm2 is promising to promote early bone healing.
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Metadata
Title
In vivo efficacy of low-level laser therapy on bone regeneration
Authors
Birtan Tolga Yılmaz
Abdullah C. Akman
Alper Çetinkaya
Can Colak
Benay Yıldırım
Özlem Özer Yücel
Güliz N. Güncü
Rahime M. Nohutcu
Publication date
13-01-2022
Publisher
Springer London
Keyword
Laser
Published in
Lasers in Medical Science / Issue 4/2022
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-021-03487-8

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