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01-04-2021 | Original Article

Effects of photobiomodulation on cellular viability and cancer stem cell phenotype in oral squamous cell carcinoma

Authors: Ana Melissa Ccopa Ibarra, Mônica Pereira Garcia, Marlene Ferreira, Daniela de Fátima Teixeira da Silva, Christiane Pavani, Raquel Agnelli Mesquita-Ferrari, Kristianne Porta Santos Fernandes, Fabio Daumas Nunes, Maria Fernanda Setúbal Destro Rodrigues

Published in: Lasers in Medical Science | Issue 3/2021

Abstract

Oral squamous cell carcinoma (OSCC) is the most common head and neck malignancy; it has been shown that cancer stem cells (CSC) are present in OSCC and associated with tumor growth, invasion, metastasis, and therapeutic resistance. Photobiomodulation (PBM) is an alternative tool for oncologic treatment adverse effects such as oral mucositis (OM); however, controversy exists regarding the undesirable effects of PBM on tumor or CSC. This study aimed to evaluate in vitro, the effects of PBM, with the same dosimetric parameters as those used in the clinic for OM prevention and treatment, on OSCC cellular viability, as well as PBM’s effect on CSC properties and its phenotype. OSCC cell lines were submitted to single or daily PBM with 3 J/cm² and 6 J/cm² and then the cellular viability was evaluated by MTT, NRU (neutral red uptake), and CVS (crystal violet staining). The CSC populations were evaluated by clonogenic formation assay, flow cytometry, and RT-qPCR. The single PBM with the 3 J/cm² group was associated with increased cellular viability. Daily PBM with 3 J/cm² and 6 J/cm² was associated with a significant decrease in cellular viability. Additionally, daily PBM was not able to promote CSC self-renewal or the CD44^high/ESA^low and CD44^high/ESA^high cellular phenotypes. Moreover, a decrease in the number of spheres and in the expression of the CSC related gene BMI1 was observed after daily PBM with 6 J/cm². Daily PBM with 3 J/cm² and 6 J/cm² showed an inhibitory effect on cellular viability and was not able to promote the CSC self-renewal or phenotype.

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Title: Effects of photobiomodulation on cellular viability and cancer stem cell phenotype in oral squamous cell carcinoma
Authors: Ana Melissa Ccopa Ibarra
Mônica Pereira Garcia
Marlene Ferreira
Daniela de Fátima Teixeira da Silva
Christiane Pavani
Raquel Agnelli Mesquita-Ferrari
Kristianne Porta Santos Fernandes
Fabio Daumas Nunes
Maria Fernanda Setúbal Destro Rodrigues
Publication date: 01-04-2021
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 3/2021
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-020-03131-x

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Effects of photobiomodulation on cellular viability and cancer stem cell phenotype in oral squamous cell carcinoma

Abstract

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Abstract

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