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Lasers in Medical Science
Published in: Lasers in Medical Science 4/2014

01-07-2014 | Original Article

Low-level laser therapy promotes proliferation and invasion of oral squamous cell carcinoma cells

Authors: Águida Cristina Gomes Henriques, Fernanda Ginani, Ruth Medeiros Oliveira, Tatjana Souza Lima Keesen, Carlos Augusto Galvão Barboza, Hugo Alexandre Oliveira Rocha, Jurema Freire Lisboa de Castro, Ricardo Della Coletta, Roseana de Almeida Freitas

Published in: Lasers in Medical Science | Issue 4/2014

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Abstract

Low-level laser therapy (LLLT) has been shown to be effective in promoting cell proliferation. There is speculation that the biostimulatory effect of LLLT causes undesirable enhancement of tumor growth in neoplastic diseases since malignant cells are more susceptible to proliferative stimuli. This study evaluated the effects of LLLT on proliferation, invasion, and expression of cyclin D1, E-cadherin, β-catenin, and MMP-9 in a tongue squamous carcinoma cell line (SCC25). Cells were irradiated with a diode laser (660 nm) using two energy densities (0.5 and 1.0 J/cm2). The proliferative potential was assessed by cell growth curves and cell cycle analysis, whereas the invasion of cells was evaluated using a Matrigel cell invasion assay. Expression of cyclin D1, E-cadherin, β-catenin, and MMP-9 was analyzed by immunofluorescence and flow cytometry and associated with the biological activities studied. LLLT induced significantly the proliferation of SCC25 cells at 1.0 J/cm2, which was accomplished by an increase in the expression of cyclin D1 and nuclear β-catenin. At 1.0 J/cm2, LLLT significantly reduced E-cadherin and induced MMP-9 expression, promoting SCC25 invasion. The results of this study demonstrated that LLLT exerts a stimulatory effect on proliferation and invasion of SCC25 cells, which was associated with alterations on expression of proteins studied.
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Metadata
Title
Low-level laser therapy promotes proliferation and invasion of oral squamous cell carcinoma cells
Authors
Águida Cristina Gomes Henriques
Fernanda Ginani
Ruth Medeiros Oliveira
Tatjana Souza Lima Keesen
Carlos Augusto Galvão Barboza
Hugo Alexandre Oliveira Rocha
Jurema Freire Lisboa de Castro
Ricardo Della Coletta
Roseana de Almeida Freitas
Publication date
01-07-2014
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 4/2014
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-014-1535-2

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