Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
Lasers in Medical Science
Published in: Lasers in Medical Science 3/2021

01-04-2021 | Laser | Original Article

Laser and LED photobiomodulation effects in osteogenic or regular medium on rat calvaria osteoblasts obtained by newly forming bone technique

Authors: Matheus Völz Cardoso, Rebeca do Vale Placa, Adriana Campos Passanezi Sant’Ana, Sebastião Luiz Aguiar Greghi, Mariana Schutzer Ragghianti Zangrando, Maria Lucia Rubo de Rezende, Rodrigo Cardoso Oliveira, Carla Andreotti Damante

Published in: Lasers in Medical Science | Issue 3/2021

Login to get access

Abstract

The purposes of this study are to evaluate the effects of photobiomodulation (PBM) with laser and LED on rat calvaria osteoblasts (rGO lineage), cultured in osteogenic (OST) or regular (REG) medium, after induction of a quiescent state and to test if PBM is capable of osteogenic induction and if there is a sum of effects when combining OST medium with PBM. Before irradiation, the cells were put in a quiescent state (1% FBS) 24 h, when red (AlGaInP—660 nm) and infrared laser (GaAlAs—808 nm) and LED (637 ± 15 nm) were applied. The groups were as follows: red laser (RL3—5 J/cm2, 3 s and RL5—8.3 J/cm2, 5 s, 1.66 W/cm2); infrared laser (IrL3—5 J/cm2, 3 s and IrL5–8.3 J/cm2, 5 s); LED (LED3—3 s and LED5—5 s, 0.02 J/cm2, 0.885 W/cm2); positive (C+, 10% FBS) and negative control (C−, 1% FBS). For alkaline phosphatase (ALP) and mineralization assays, the cells were cultured in REG (DMEM 10% FBS) and OST medium (DMEM 10% FBS, 50 μg/mL ascorbic acid, 10 mM β-glycerophosphate). Statistical analysis was performed using ANOVA and Tukey’s tests (p < 0.05). RL5 and LED5 increased proliferation, in vitro wound closure, ALP, and mineralization in rGO cells (p < 0.05). PBM with red laser and LED induced mineralization by itself, without osteogenic medium, not observed for infrared laser (p < 0.05). A sum of effects was observed in osteogenic medium and PBM by infrared, red laser, and LED (5 s). Red laser and LED increased proliferation, migration, and secretory phases in rGO cells in a dose-dependent manner. PBM with red laser and LED promotes osteogenic induction by itself. PBM with infrared laser and osteogenic medium potentializes mineralization.
Literature
1.
Hosseinpour S, Fekrazad R, Arany PR, Qingsong Y (2019) Molecular impacts of photobiomodulation on bone regeneration: a systematic review. Prog Biophys Mol Biol 149:147–159PubMedCrossRef
2.
3.
4.
AlGhamdi KM, Kumar A, Moussa NA (2012) Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci 27:237–249PubMedCrossRef
5.
Noba C, Mello-Moura ACV, Gimenez T, Tedesco TK, Moura-Netto C (2018) Laser for bone healing after oral surgery: systematic review. Lasers Med Sci 33:667–674PubMedCrossRef
6.
Passanezi E, Damante CA, de Rezende MLR, Greghi SLA (2015) Lasers in periodontal therapy. Periodontol 2000 67:268–291PubMedCrossRef
7.
Stein A, Benayahu D, Maltz L, Oron U (2005) Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg 23:161–166PubMedCrossRef
8.
Ozawa Y, Shimizu N, Kariya G, Abiko Y (1998) Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone 22:347–354PubMedCrossRef
9.
Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K (2005) Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. Biomaterials 26:3503–3509PubMedCrossRef
10.
Fujihara NA, Hiraki KR, Marques MM (2006) Irradiation at 780 nm increases proliferation rate of osteoblasts independently of dexamethasone presence. Lasers Surg Med 38:332–336PubMedCrossRef
11.
Dortbudak O, Haas R, Mallath-Pokorny G (2000) Biostimulation of bone marrow cells with a diode soft laser. Clin Oral Implants Res 11(6):540–545PubMedCrossRef
12.
Deana AM, de Souza AM, Teixeira VP, Mesquita-Ferrari RA, Bussadori SK, Fernandes KPS (2018) The impact of photobiomodulation on osteoblast-like cell: a review. Lasers Med Sci 33:1147–1158PubMedCrossRef
13.
Oliveira FA, Matos AA, Santesso MR et al (2016) Low intensity lasers differently induce primary human osteoblast proliferation and differentiation. J Photochem Photobiol B 163:14–21PubMedCrossRef
14.
Li WT, Leu YC, Wu JL (2010) Red-light light-emitting diode irradiation increases the proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells. Photomed Laser Surg 28:S157–S165PubMedCrossRef
15.
Damante CA, De Micheli G, Miyagi SP, Feist IS, Marques MM (2009) Effect of laser phototherapy on the release of fibroblast growth factors by human gingival fibroblasts. Lasers Med Sci 24:885–891PubMedCrossRef
16.
17.
Almeida-Lopes L, Rigau J, Zangaro RA, Guidugli-Neto J, Jaeger MM (2001) Comparison of the low level laser therapy effects on cultured human gingival fibroblasts proliferation using different irradiance and same fluence. Lasers Surg Med 29:179–184PubMedCrossRef
18.
Campisi J, Morreo G, Pardee AB (1984) Kinetics of G1 transit following brief starvation for serum factors. Exp Cell Res 152:459–466PubMedCrossRef
19.
Karu T (1999) A suitable model for wound healing: how many times are we to stumble over the same block? Lasers Surg Med 25:283PubMedCrossRef
20.
Passanezi E, Janson WA, Nahas D, Campos Junior A (1989) Newly forming bone autografts to treat periodontal infrabony pockets: clinical and histological events. Int J Periodontics Restorative Dent 9:140–153PubMed
21.
Sant'Ana ACP, Damante CA, Frias Martinez MA et al (2018) Isolation and characterization of progenitor cells from surgically created early healing alveolar defects in humans: a preliminary study. J Periodontol 89:1326–1333PubMedCrossRef
22.
Armelin HA, Armelin MC, Kelly K et al (1984) Functional role for c-myc in mitogenic response to platelet-derived growth factor. Nature 310(5979):655–660PubMedCrossRef
23.
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65(1–2):55–63CrossRefPubMed
24.
Damante CA, Marques MM (2014) Laser power loss through polystyrene plates for cell culture. Lasers Med Sci 29(1):373PubMedCrossRef
25.
Hamblin MR (2019) How to write a good photobiomodulation article. Photobiomodul Photomed Laser Surg 37(6):325–326PubMedCrossRef
26.
27.
Kueng W, Silber E, Eppenberger U (1989) Quantification of cells cultured on 96-well plates. Anal Biochem 182(1):16–19PubMedCrossRef
28.
Pagin MT, de Oliveira FA, Oliveira RC et al (2014) Laser and light-emitting diode effects on pre-osteoblast growth and differentiation. Lasers Med Sci 29(1):55–59PubMedCrossRef
29.
30.
Hoang AM, Oates TW, Cochran DL (2000) In vitro wound healing responses to enamel matrix derivative. J Periodontol 71(8):1270–1277PubMedCrossRef
31.
Liang CC, Park AY, Guan JL (2007) In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc 2(2):329–333PubMedCrossRef
32.
33.
Zambuzzi WF, Granjeiro JM, Parikh K et al (2008) Modulation of Src activity by low molecular weight protein tyrosine phosphatase during osteoblast differentiation. Cell Physiol Biochem 22(5–6):497–506PubMedCrossRef
34.
Heo JH, Choi JH, Kim IR, Park BS, Kim YD (2018) Combined treatment with low-level laser and rhBMP-2 promotes differentiation and mineralization of osteoblastic cells under hypoxic stress. Tissue Eng Regen Med 15(6):793–801PubMedPubMedCentralCrossRef
35.
Pinheiro AL, Gerbi ME (2006) Photoengineering of bone repair processes. Photomed Laser Surg 24(2):169–178PubMedCrossRef
36.
Fujimoto K, Kiyosaki T, Mitsui N et al (2010) Low-intensity laser irradiation stimulates mineralization via increased BMPs in MC3T3-E1 cells. Lasers Surg Med 42(6):519–526PubMedCrossRef
37.
Petri AD, Teixeira LN, Crippa GE et al (2010) Effects of low-level laser therapy on human osteoblastic cells grown on titanium. Braz Dent J 21(6):491–498PubMedCrossRef
38.
Mergoni G, Vescovi P, Belletti S, Uggeri J, Nammour S, Gatti R (2018) Effects of 915 nm laser irradiation on human osteoblasts: a preliminary in vitro study. Lasers Med Sci 33(6):1189–1195PubMedCrossRef
39.
Medina-Huertas R, Manzano-Moreno FJ, De Luna-Bertos E et al (2014) The effects of low-level diode laser irradiation on differentiation, antigenic profile, and phagocytic capacity of osteoblast-like cells (MG-63). Lasers Med Sci 29(4):1479–1484PubMed
40.
41.
da Silva AP, Petri AD, Crippa GE, Stuani AS, Rosa AL, Stuani MB (2012) Effect of low-level laser therapy after rapid maxillary expansion on proliferation and differentiation of osteoblastic cells. Lasers Med Sci 27(4):777–783PubMedCrossRef
42.
Coombe AR, Ho CT, Darendeliler MA et al (2001) The effects of low level laser irradiation on osteoblastic cells. Clin Orthod Res 4(1):3–14PubMedCrossRef
43.
44.
Aihara N, Yamaguchi M, Kasai K (2006) Low-energy irradiation stimulates formation of osteoclast-like cells via RANK expression in vitro. Lasers Med Sci 21(1):24–33PubMedCrossRef
45.
Pinheiro AL, Santos NR, Oliveira PC et al (2013) The efficacy of the use of IR laser phototherapy associated to biphasic ceramic graft and guided bone regeneration on surgical fractures treated with wire osteosynthesis: a comparative laser fluorescence and Raman spectral study on rabbits. Lasers Med Sci 28(3):815–822PubMedCrossRef
46.
Aghajanian P, Hall S, Wongworawat MD, Mohan S (2015) The roles and mechanisms of actions of vitamin C in bone: new developments. J Bone Miner Res 30(11):1945–1955PubMedCrossRef
47.
Chung CH, Golub EE, Forbes E, Tokuoka T, Shapiro IM (1992) Mechanism of action of beta-glycerophosphate on bone cell mineralization. Calcif Tissue Int 51(4):305–311PubMedCrossRef
48.
Noh AL, Yim M (2011) Beta-glycerophosphate accelerates RANKL-induced osteoclast formation in the presence of ascorbic acid. Pharmazie 66(3):195–200PubMed
49.
50.
Jeong SY, Hong JU, Song JM et al (2018) Combined effect of recombinant human bone morphogenetic protein-2 and low level laser irradiation on bisphosphonate-treated osteoblasts. J Korean Assoc Oral Maxillofac Surg 44(6):259–268PubMedPubMedCentralCrossRef
51.
Chintavalakorn R, Khantachawana A, Viravaidya-Pasuwat K, Santiwong P, Surarit R (2017) In vitro effects of mechanical stimulation and photobiomodulation on osteoblastic cell function: a proof of concept study. Pediatr Dent J 27(1):29–41CrossRef
52.
53.
Jackson RA, Kumarasuriyar A, Nurcombe V, Cool SM (2006) Long-term loading inhibits ERK1/2 phosphorylation and increases FGFR3 expression in MC3T3-E1 osteoblast cells. J Cell Physiol 209(3):894–904PubMedCrossRef
54.
Renno AC, McDonnell PA, Parizotto NA, Laakso EL (2007) The effects of laser irradiation on osteoblast and osteosarcoma cell proliferation and differentiation in vitro. Photomed Laser Surg 25(4):275–280PubMedCrossRef
55.
Smith RJ, Birndorf M, Gluck G, Hammond D, Moore WD (1992) The effect of low-energy laser on skin-flap survival in the rat and porcine animal models. Plast Reconstr Surg 89(2):306–310PubMedCrossRef
56.
57.
Pinheiro ALB, Soares LGP, da Silva ACP et al (2018) Laser/LED phototherapy on the repair of tibial fracture treated with wire osteosynthesis evaluated by Raman spectroscopy. Lasers Med Sci 33(8):1657–1666PubMedCrossRef
Metadata
Title
Laser and LED photobiomodulation effects in osteogenic or regular medium on rat calvaria osteoblasts obtained by newly forming bone technique
Authors
Matheus Völz Cardoso
Rebeca do Vale Placa
Adriana Campos Passanezi Sant’Ana
Sebastião Luiz Aguiar Greghi
Mariana Schutzer Ragghianti Zangrando
Maria Lucia Rubo de Rezende
Rodrigo Cardoso Oliveira
Carla Andreotti Damante
Publication date
01-04-2021
Publisher
Springer London
Keyword
Laser
Published in
Lasers in Medical Science / Issue 3/2021
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-020-03056-5

Other articles of this Issue 3/2021

Lasers in Medical Science 3/2021 Go to the issue

Original Article

Investigation of LED-based photodynamic therapy efficiency on breast cancer cells

Brief Report

Impact of 970 nm photobiomodulation therapy on wound healing in cellular models of hidradenitis suppurativa

Original Article

Influence of photodynamic therapy on the periodontitis-induced bone resorption in rat

Original Article

Efficacy of non-ablative fractional 1440-nm laser therapy for treatment of facial acne scars in patients with rosacea: a prospective, interventional study

Original Article

Thermal field and tissue damage analysis of moving laser in cancer thermal therapy

Original Article

Effects of photobiomodulation on cellular viability and cancer stem cell phenotype in oral squamous cell carcinoma