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Lasers in Medical Science
Published in: Lasers in Medical Science 3/2020

01-04-2020 | Laser | Original Article

Photobiomodulation therapy compensate the impairments of diabetic bone marrow mesenchymal stem cells

Authors: Fatemeh Zare, Mohammad Bayat, Abbas Aliaghaei, Abbas Piryaei

Published in: Lasers in Medical Science | Issue 3/2020

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Abstract

Pathophysiologic conditions associated with diabetes mellitus affect mesenchymal stem cells (MSCs), and this phenomenon may lead to some diabetic secondary complications. The present study was conducted to evaluate the impact of photobiomodulation (PBM) on rat diabetic MSC (DMSC) behavior in vitro. For the purpose of PBM, we used helium-neon laser with a wavelength of 632.8 nm at three different energy densities (0.5, 1, 2 J/cm2) and radiation periodicity of once, twice, and thrice. The survival, proliferation, and apoptosis in the normal MSCs (NMSCs), DMSCs, and diabetic MSCs, which were laser irradiated (DMSCs+L), were assessed using MTT assay, Ki67 immunofluorescence staining, and TUNEL assay, respectively. Our results demonstrated that DMSCs have significantly lower survival (P < 0.05) and proliferation rates (P < 0.001), and dramatically higher population doubling time (PDT, P < 0.001) and apoptosis rates (P < 0.001) as compared to NMSCs. Moreover, PBM with energy density of 1 J/cm2 and the periodicity of 1 or 2 times could improve diabetic MSC capabilities in the term of survival, proliferation, and apoptosis. Considering these findings, it is suggested that PBM could improve the ability of diabetic MSCs in vitro prior to transplantation or may rise their capabilities in their native niche in vivo.
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Metadata
Title
Photobiomodulation therapy compensate the impairments of diabetic bone marrow mesenchymal stem cells
Authors
Fatemeh Zare
Mohammad Bayat
Abbas Aliaghaei
Abbas Piryaei
Publication date
01-04-2020
Publisher
Springer London
Keyword
Laser
Published in
Lasers in Medical Science / Issue 3/2020
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-019-02844-y

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