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Lasers in Medical Science
Published in: Lasers in Medical Science 1/2019

01-02-2019 | Original Article

Effects of Nd:YAG low-level laser irradiation on cultured human osteoblasts migration and ATP production: in vitro study

Authors: Yuji Tsuka, Ryo Kunimatsu, Hidemi Gunji, Kengo Nakajima, Aya Kimura, Tomoka Hiraki, Ayaka Nakatani, Kotaro Tanimoto

Published in: Lasers in Medical Science | Issue 1/2019

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Abstract

Low-level laser therapy has become one of the fastest growing fields of medicine in recent years. Many in vivo and in vitro studies have shown that laser irradiation activates a range of cellular processes in a variety of cell types and can promote tissue repair. However, few in vitro experiments have evaluated the effects of laser irradiation on cells in real time. The purpose of this study was to examine the effects of neodymium-doped yttrium aluminum garnet (Nd:YAG) laser irradiation on the migration of cultured human osteoblasts. A dedicated 96-well plate was used, and confluent cultures of the human osteoblast-like cell line, Saos-2, were injured with a wound maker. The wounded cells were then exposed to the Nd:YAG laser (wavelength of 1064 nm) for 60 s at 0.3 W (10 pps, 30 mJ). The total energy density was about 10.34 J/cm2. Images of the wounds were automatically acquired inside the CO2 incubator by the IncuCyte ZOOM™ software. In addition, after laser irradiation, the production of adenosine triphosphate (ATP) was measured using the CellTiter-Glo™ Luminescent Cell Viability Assay. Migration of cells from the border of the original scratch zone was accelerated by laser irradiation. In addition, compared with the control group, significant enhancement of ATP production was observed in the irradiated group. The present study showed that Nd:YAG laser irradiation (wavelength of 1064 nm, 0.3 W, 10 pps, 30 mJ, 10.34 J/cm2, irradiation time 60 s) may contribute to the regeneration of bone tissues owing to enhanced osteoblast cell migration.
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Metadata
Title
Effects of Nd:YAG low-level laser irradiation on cultured human osteoblasts migration and ATP production: in vitro study
Authors
Yuji Tsuka
Ryo Kunimatsu
Hidemi Gunji
Kengo Nakajima
Aya Kimura
Tomoka Hiraki
Ayaka Nakatani
Kotaro Tanimoto
Publication date
01-02-2019
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 1/2019
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-018-2586-6

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