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Lasers in Medical Science
Published in: Lasers in Medical Science 2/2012

01-03-2012 | Original Article

The effects of low-level laser irradiation on differentiation and proliferation of human bone marrow mesenchymal stem cells into neurons and osteoblasts—an in vitro study

Authors: Masoud Soleimani, Ehsan Abbasnia, Mehdi Fathi, Hedayat Sahraei, Yashar Fathi, Gholamreza Kaka

Published in: Lasers in Medical Science | Issue 2/2012

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Abstract

Bone marrow-derived mesenchymal stem cells (BMSCs) are promising for use in regenerative medicine. Several studies have shown that low-level laser irradiation (LLLI) could affect the differentiation and proliferation of MSCs. The aim of this study was to examine the influence of LLLI at different energy densities on BMSCs differentiation into neuron and osteoblast. Human BMSCs were cultured and induced to differentiate to either neuron or osteoblast in the absence or presence of LLLI. Gallium aluminum arsenide (GaAlAs) laser irradiation (810 nm) was applied at days 1, 3, and 5 of differentiation process at energy densities of 3 or 6 J/cm2 for BMSCs being induced to neurons, and 2 or 4 J/cm2 for BMSCs being induced to osteoblasts. BMSCs proliferation was evaluated by MTT assay on the seventh day of differentiation. BMSCs differentiation to neurons was assessed by immunocytochemical analysis of neuron-specific enolase on the seventh day of differentiation. BMSCs differentiation to osteoblast was tested on the second, fifth, seventh, and tenth day of differentiation via analysis of alkaline phosphatase (ALP) activity. LLLI promoted BMSCs proliferation significantly at all energy densities except for 6 J/cm2 in comparison to control groups on the seventh day of differentiation. LLLI at energy densities of 3 and 6 J/cm2 dramatically facilitated the differentiation of BMSCs into neurons (p < 0.001). Also, ALP activity was significantly enhanced in irradiated BMSCs differentiated to osteoblast on the second, fifth, seventh, and tenth day of differentiation (p < 0.001 except for the second day). Using LLLI at 810 nm wavelength enhances BMSCs differentiation into neuron and osteoblast in the range of 2–6 J/cm2, and at the same time increases BMSCs proliferation (except for 6 J/cm2). The effect of LLLI on differentiation and proliferation of BMSCs is dose-dependent. Considering these findings, LLLI could improve current in vitro methods of differentiating BMSCs prior to transplantation.
Literature
1.
Caplan AI (2005) Review: Mesenchymal stem cells: Cell-based reconstructive therapy in orthopedics. Tissue Eng 11:1198–1211PubMedCrossRef
2.
Meirelles Lda S, Fontes AM, Covas DT, Caplan AI (2009) Mechanisms involved in the therapeutic properties of mesenchymal stem cells. Cytokine Growth Factor Rev 20:419–427PubMedCrossRef
3.
Wagner J, Kean T, Young R, Dennis JE, Caplan AI (2009) Optimizing mesenchymal stem cell-based therapeutics. Curr Opin Biotechnol 20:531–536PubMedCrossRef
4.
Filip S, Mokry J, Hruska I (2003) Adult stem cells and their importance in cell therapy. Folia Biol (Praha) 49:9–14
5.
Dennis JE, Caplan AI (2004) Bone marrow mesenchymal stem cells. In: Sell S (ed) Stem cells handbook. Humana Press Inc, Totowa, pp 107–117
6.
Mester E, Spiry T, Szende B, Tota JG (1971) Effect of laser rays on wound healing. Am J Surg 122:532–535PubMedCrossRef
7.
Moshkovska T, Mayberry J (2005) It is time to test low-level laser therapy in Great Britain. Postgrad Med J 81:436–441PubMedCrossRef
8.
Khadra M, Lyngstadaas SP, Haanas HR, Mustafa K (2005) Determining optimal dose of laser therapy for attachment and proliferation of human oral fibroblasts cultured on titanium implant material. J Biomed Mater Res 73:55–62CrossRef
9.
Grossman N, Schneid N, Reuveni H, Halevy S, Lubart R (1998) 780-nm low-power diode laser irradiation stimulates proliferation of keratinocyte cultures: involvement of reactive oxygen species. Lasers Surg Med 22:212–218PubMedCrossRef
10.
Stein A, Benayahu D, Maltz L, Oron U (2005) Low-level laser irradiation promotes proliferation and differentiation of human osteoblasts in vitro. Photomed Laser Surg 23:161–166PubMedCrossRef
11.
Stadler I, Evans R, Kolb B, Naim JO, Narayan V et al (2000) In vitro effects of low-level laser irradiation at 660 nm on peripheral blood lymphocytes. Lasers Surg Med 27:255–261PubMedCrossRef
12.
Renno AC, McDonnell PA, Parizotto NA, Laakso EL (2007) The effects of laser irradiation on osteoblast and osteosarcoma cell proliferation and differentiation in vitro. Photomed Laser Surg 25:275–280PubMedCrossRef
13.
Aihara N, Yamaguchi M, Kasai K (2006) Low-energy irradiation stimulates formation of osteoclast-like cells via RANK expression in vitro. Lasers Med Sci 21:24–33PubMedCrossRef
14.
Khadra M, Lyngstadaas SP, Haanaes HR, Mustafa K (2005) Effect of laser therapy on attachment, proliferation and differentiation of human osteoblast-like cells cultured on titanium implant material. Biomaterials 26:3503–3509PubMedCrossRef
15.
Horva´t-Karajz K, Balogh S (2009) In vitro effect of carboplatin, cytarabine, paclitaxel, vincristine, and low-power laser irradiation on murine mesenchymal stem cells. Lasers Surg Med 41:463–469
16.
Hou Jian-feng, Zhang H, Yuan X, Li J, Wei Ying-jie et al (2008) In vitro effects of low-level laser irradiation for bone marrow mesenchymal stem cells: proliferation, growth factors secretion and myogenic differentiation. Lasers Surg Med 40:726–733PubMedCrossRef
17.
Abramovitch-Gottlib L, Gross T, Naveh D, Geresh S, Rosenwaks S et al (2005) Low-level laser irradiation stimulates osteogenic phenotype of mesenchymal stem cells seeded on a three-dimensional biomatrix. Lasers Med Sci 20:138–146PubMedCrossRef
18.
Mvula B, Mathope T, Moore T, Abrahamse H (2008) The effect of low-level laser irradiation on adult human adipose derived stem cells. Lasers Med Sci 23:277–282PubMedCrossRef
19.
Eduardo FdeP, Bueno DF, de Freitas PM, Marques MM, Passos-Bueno MR et al (2008) Stem cell proliferation under low-intensity laser irradiation: a preliminary study. Lasers Surg Med 40:433–438CrossRef
20.
Choong P-F, P-L Mok, S-K Cheong, C-F Leong, K-Y Then (2007) Generating neuron-like cells from BM-derived mesenchymal stromal cells in vitro. Cytotherapy 9:170–183PubMedCrossRef
21.
Choi CB, Cho YK, Bhanu Prakash KV, Jee BK, Han CW et al. Analysis of neuron-like differentiation of human bone marrow mesenchymal stem cells
22.
Stein E, Koehn J, Sutter W, Wendtlandt G, Wanschitz F et al (2008) Initial effects of low-level laser therapy on growth and differentiation of human osteoblast-like cells. Wien Klin Wochenschr 120:112–117PubMedCrossRef
23.
Bouvet-Gerbettaz S, Merigo E, Carle J-PRGF, Rochet N (2009) Effects of low-level laser therapy on proliferation and differentiation of murine bone marrow cells into osteoblasts and osteoclasts. Lasers Surg Med 41:291–297PubMedCrossRef
24.
Karu Tl (1990) Yearly review: effect of visible radiation on cultured cells. Photochem Photobiol 52:1089–1098PubMedCrossRef
25.
O'Kane S, Shields TD, Gilmore WS, Allen JM (1994) Low-intensity laser irradiation inhibits tritiated thymidine incorporation in the hemopoietic cell lines HL-60 and U937 14:34–39
Metadata
Title
The effects of low-level laser irradiation on differentiation and proliferation of human bone marrow mesenchymal stem cells into neurons and osteoblasts—an in vitro study
Authors
Masoud Soleimani
Ehsan Abbasnia
Mehdi Fathi
Hedayat Sahraei
Yashar Fathi
Gholamreza Kaka
Publication date
01-03-2012
Publisher
Springer-Verlag
Published in
Lasers in Medical Science / Issue 2/2012
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-011-0930-1

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