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01-12-2015 | Original Article

Effect of low-level laser irradiation on proliferation and viability of human dental pulp stem cells

Authors: Ivana Maria Zaccara, Fernanda Ginani, Haroldo Gurgel Mota-Filho, Águida Cristina Gomes Henriques, Carlos Augusto Galvão Barboza

Published in: Lasers in Medical Science | Issue 9/2015

Abstract

A positive effect of low-level laser irradiation (LLLI) on the proliferation of some cell types has been observed, but little is known about its effect on dental pulp stem cells (DPSCs). The aim of this study was to identify the lowest energy density able to promote the proliferation of DPSCs and to maintain cell viability. Human DPSCs were isolated from two healthy third molars. In the third passage, the cells were irradiated or not (control) with an InGaAlP diode laser at 0 and 48 h using two different energy densities (0.5 and 1.0 J/cm²). Cell proliferation and viability and mitochondrial activity were evaluated at intervals of 24, 48, 72, and 96 h after the first laser application. Apoptosis- and cell cycle-related events were analyzed by flow cytometry. The group irradiated with an energy density of 1.0 J/cm² exhibited an increase of cell proliferation, with a statistically significant difference (p < 0.05) compared to the control group at 72 and 96 h. No significant changes in cell viability were observed throughout the experiment. The distribution of cells in the cell cycle phases was consistent with proliferating cells in all three groups. We concluded that LLLI, particularly a dose of 1.0 J/cm², contributed to the growth of DPSCs and maintenance of its viability. This fact indicates this therapy to be an important future tool for tissue engineering and regenerative medicine involving stem cells.

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Title: Effect of low-level laser irradiation on proliferation and viability of human dental pulp stem cells
Authors: Ivana Maria Zaccara
Fernanda Ginani
Haroldo Gurgel Mota-Filho
Águida Cristina Gomes Henriques
Carlos Augusto Galvão Barboza
Publication date: 01-12-2015
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 9/2015
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-015-1803-9

At a glance: The STEP trials

Springer Medicine

Effect of low-level laser irradiation on proliferation and viability of human dental pulp stem cells

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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