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Lasers in Medical Science
Published in: Lasers in Medical Science 3/2008

01-07-2008 | Original Article

The effect of low level laser irradiation on adult human adipose derived stem cells

Authors: B. Mvula, T. Mathope, T. Moore, H. Abrahamse

Published in: Lasers in Medical Science | Issue 3/2008

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Abstract

This study investigated the effect of low level laser irradiation on primary cultures of adult human adipose derived stem cells (ADSC) using a 635-nm diode laser, at 5 J/cm2 with a power output of 50.2 mW and a power density of 5.5 mW/cm2. Cellular morphology did not appear to change after irradiation. Using the trypan blue exclusion test, the cellular viability of irradiated cells increased by 1% at 24 h and 1.6% at 48 h but was not statistically significant. However, the increase of cellular viability as measured by ATP luminescence was statistically significant at 48 h (p < 0.05). Proliferation of irradiated cells, measured by optical density, resulted in statistically significant increases in values compared to nonirradiated cells (p < 0.05) at both time points. Western blot analysis and immunocytochemical labeling indicated an increase in the expression of stem cell marker β1-integrin after irradiation. These results indicate that 5 J/cm2 of laser irradiation can positively affect human adipose stem cells by increasing cellular viability, proliferation, and expression of β1-integrin.
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Metadata
Title
The effect of low level laser irradiation on adult human adipose derived stem cells
Authors
B. Mvula
T. Mathope
T. Moore
H. Abrahamse
Publication date
01-07-2008
Publisher
Springer-Verlag
Published in
Lasers in Medical Science / Issue 3/2008
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-007-0479-1

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