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01-03-2014 | Original Article

The role of transforming growth factor β1 in fractional laser resurfacing with a carbon dioxide laser

Authors: Xia Jiang, Hongmei Ge, Chuanqing Zhou, Xinyu Chai, Hui Deng

Published in: Lasers in Medical Science | Issue 2/2014

Abstract

The aim of this study was to investigate the role of transforming growth factor β1 in mechanisms of cutaneous remodeling induced by fractional carbon dioxide laser treatment. The dorsal skin of Kunming mice was exposed to a single-pass fractional CO₂ laser treatment. Biopsies were taken at 1 h and at 1, 3, 7, 14, 21, 28, and 56 days after treatment. Transforming growth factor (TGF) β1 expression in skin samples was evaluated by ELISA, dermal thickness by hematoxylin–eosin staining, collagen and elastic fibers by Ponceau S and Victoria blue double staining, and types I and III collagens by ELISA. The level of TGF β1 in the laser-treated areas of skin was significantly increased compared with that in the control areas on days 1 (p < 0.05), 3 (p < 0.01), and 7 (p < 0.05) and then decreased by day 14 after treatment, at which time it had returned to the baseline level. Dermal thickness and the amount of type I collagen of the skin of the laser-treated areas had increased significantly (p < 0.05) compared with that in control areas on days 28 and 56. Fibroblast proliferation showed a positive correlation with TGF β1 expression during the early stages (r = 0.789, p < 0.01), and there was a negative correlation between the level of TGF β1 and type I collagen in the late stages, after laser treatment (r = −0.546, p < 0.05). TGF β1 appears to be an important factor in fractional laser resurfacing.

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Title: The role of transforming growth factor β1 in fractional laser resurfacing with a carbon dioxide laser
Authors: Xia Jiang
Hongmei Ge
Chuanqing Zhou
Xinyu Chai
Hui Deng
Publication date: 01-03-2014
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 2/2014
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-013-1383-5

At a glance: The STEP trials

Springer Medicine

The role of transforming growth factor β1 in fractional laser resurfacing with a carbon dioxide laser

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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