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European Journal of Clinical Microbiology & Infectious Diseases

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01-01-2022 | Tick-Borne Encephalitis | Brief Report

CXCL13 in laboratory diagnosis of Lyme neuroborreliosis—the performance of the recomBead and ReaScan CXCL13 assays in human cerebrospinal fluid samples

Authors: Sofie Haglund, Malin Lager, Paula Gyllemark, Gärda Andersson, Oskar Ekelund, Martin Sundqvist, Anna J. Henningsson

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 1/2022

Abstract

The chemokine CXCL13 is used as complement to serology in the diagnostics of Lyme neuroborreliosis (LNB). We evaluated and compared the semi-quantitative, cassette-based ReaScan CXCL13 assay with the quantitative recomBead CXCL13 assay using a collection of 209 cerebrospinal fluid samples. The categorical agreement between results interpreted as negative, grey zone, and positive by the two methods was 87%. The diagnostic sensitivity was higher using the recomBead assay, whereas specificity was higher using ReaScan. Few manual steps, and a short turn-around time with no batching of samples makes the ReaScan CXCL13 assay an attractive complement to serology in the diagnostics of LNB.

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Title: CXCL13 in laboratory diagnosis of Lyme neuroborreliosis—the performance of the recomBead and ReaScan CXCL13 assays in human cerebrospinal fluid samples
Authors: Sofie Haglund
Malin Lager
Paula Gyllemark
Gärda Andersson
Oskar Ekelund
Martin Sundqvist
Anna J. Henningsson
Publication date: 01-01-2022
Publisher: Springer Berlin Heidelberg
Keywords: Tick-Borne Encephalitis
Neurologic Lyme Disease
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 1/2022
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-021-04350-y

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