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European Journal of Clinical Microbiology & Infectious Diseases

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01-02-2021 | Severe Acute Respiratory Syndrome Coronavirus | Review

Laboratory detection methods for the human coronaviruses

Authors: Ehsan Shabani, Sayeh Dowlatshahi, Mohammad J. Abdekhodaie

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 2/2021

Abstract

Coronaviruses are a group of envelop viruses which lead to diseases in birds and mammals as well as human. Seven coronaviruses have been discovered in humans that can cause mild to lethal respiratory tract infections. HCoV-229E, HCoV-OC43, HCoV-NL63, and HCoV-HKU1 are the low-risk members of this family and the reason for some common colds. Besides, SARS-CoV, MERS-CoV, and newly identified SARS-CoV-2, which is also known as 2019-nCoV, are the more dangerous viruses. Due to the rapid spread of this novel coronavirus and its related disease, COVID-19, a reliable, simple, fast, and low-cost detection method is necessary for patient diagnosis and tracking worldwide. Human coronaviruses detection methods were classified and presented in this article. The laboratory detection techniques include RT-PCR, RT-LAMP, electrochemical and optical biosensors for RNA detection, and whole virus or viral proteins detection assays.

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Title: Laboratory detection methods for the human coronaviruses
Authors: Ehsan Shabani
Sayeh Dowlatshahi
Mohammad J. Abdekhodaie
Publication date: 01-02-2021
Publisher: Springer Berlin Heidelberg
Keywords: Severe Acute Respiratory Syndrome Coronavirus
SARS-CoV-2
Middle Eastern Respiratory Syndrome
COVID-19
Coronavirus
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 2/2021
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-020-04001-8

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