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European Journal of Clinical Microbiology & Infectious Diseases

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01-08-2020 | Bone Infection | Brief Report

Misidentification of Cutibacterium namnetense as Cutibacterium acnes among clinical isolates by MALDI-TOF VitekMS: usefulness of gyrB sequencing and new player in bone infections

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 8/2020

Abstract

The taxonomy modification of Propionibacterium sp. with the description of new species, especially Cutibacterium namnetense, raises the question of species distribution in routine clinical samples. We performed a retrospective study during 3 years before the implementation of MALDI-TOF. Two hundred sixty-nine isolates were included in the study. MALDI-TOF identification, 16S rRNA, and new developed gyrB partial sequencings were performed. The most representative species was C. acnes in 88% of the cases, regardless of the origin of the clinical sample. Eventually, we identified three C. namnetense strains, representing a 1.1% prevalence over the period of time, including two bone infections. MALDI-TOF databases should be regularly updated to incorporate new species. gyrB sequencing constitutes a both easy and relevant method to identify Cutibacterium sp. especially C. namnetense, a new player in bone infections.

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Paugam C, Corvec S, Saint-Jean M, Le Moigne M, Khammari A, Boisrobert A et al (2017) Propionibacterium acnes phylotypes and acne severity: an observational prospective study. J Eur Acad Dermatol Venereol 31:e398–e399. https://doi.org/10.1111/jdv.14206 CrossRefPubMed

Aubin GG, Baud’huin M, Lavigne J-P, Brion R, Gouin F, Lepelletier D et al (2017) Interaction of Cutibacterium ( formerly Propionibacterium) acnes with bone cells: a step toward understanding bone and joint infection development. Sci Rep 7:42918. https://doi.org/10.1038/srep42918 CrossRefPubMedPubMedCentral

Aubin GG, Ada Da Silva G, Eishi Y, Jacqueline C, Altare F, Corvec S et al (2017) Immune discrepancies during in vitro granuloma formation in response to Cutibacterium (formerly Propionibacterium) acnes infection. Anaerobe 48:172–176. https://doi.org/10.1016/j.anaerobe.2017.08.014 CrossRefPubMed

Corvec S (2018) Clinical and biological features of Cutibacterium (formerly Propionibacterium) avidum, an underrecognized microorganism. Clin Microbiol Rev 31. https://doi.org/10.1128/CMR.00064-17

Mak TN, Yu S-H, De Marzo AM, Brüggemann H, Sfanos KS (2013) Multilocus sequence typing (MLST) analysis of Propionibacterium acnes isolates from radical prostatectomy specimens. Prostate 73:770–777. https://doi.org/10.1002/pros.22621 CrossRefPubMed

Scholz CFP, Kilian M (2016) The natural history of cutaneous propionibacteria, and reclassification of selected species within the genus Propionibacterium to the proposed novel genera Acidipropionibacterium gen. nov., Cutibacterium gen. nov. and Pseudopropionibacterium gen. nov. Int J Syst Evol Microbiol 66:4422–4432. https://doi.org/10.1099/ijsem.0.001367 CrossRefPubMed

Dekio I, Culak R, Misra R, Gaulton T, Fang M, Sakamoto M et al (2015) Dissecting the taxonomic heterogeneity within Propionibacterium acnes: proposal for Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes subsp. elongatum subsp. nov. Int J Syst Evol Microbiol 65:4776–4787. https://doi.org/10.1099/ijsem.0.000648 CrossRefPubMed

McDowell A, Barnard E, Liu J, Li H, Patrick S (2016) Proposal to reclassify Propionibacterium acnes type I as Propionibacterium acnes subsp. acnes subsp. nov. and Propionibacterium acnes type II as Propionibacterium acnes subsp. defendens subsp. nov. Int J Syst Evol Microbiol 66:5358–5365. https://doi.org/10.1099/ijsem.0.001521 CrossRefPubMedPubMedCentral

Corvec S, Luchetta J, Aubin GG (2015) Letter to the editor: is hemolysis a clinical marker of Propionibacterium acnes orthopedic infection or a phylogenetic marker? Am J Orthop Belle Mead NJ 44:E61–E62PubMed

10.

Aubin GG, Bémer P, Kambarev S, Patel NB, Lemenand O, Caillon J et al (2016) Propionibacterium namnetense sp. nov., isolated from a human bone infection. Int J Syst Evol Microbiol 3. https://doi.org/10.1099/ijsem.0.001204

11.

Aubin GG, Kambarev S, Bémer P, Lawson PA, Corvec S (2016) Draft genome sequence of highly rifampin-resistant Propionibacterium namnetense NTS 31307302T isolated from a patient with a bone infection. Genome Announc 11:4. https://doi.org/10.1128/genomeA.00819-16 CrossRef

12.

Corvec S, Guillouzouic A, Aubin GG, Touchais S, Grossi O, Gouin F et al (2018) Rifampin-resistant Cutibacterium (formerly Propionibacterium) namnetense superinfection after Staphylococcus aureus bone infection treatment. J Bone Jt Infect 3:255–257. https://doi.org/10.7150/jbji.30029 CrossRefPubMedPubMedCentral

13.

d’Ersu J, Aubin GG, Mercier P, Nicollet P, Bémer P, Corvec S (2016) Characterization of Staphylococcus caprae clinical isolates involved in human bone and joint infections, compared with goat mastitis isolates. J Clin Microbiol 54:106–113. https://doi.org/10.1128/JCM.01696-15 CrossRefPubMed

14.

Torrens C, Marí R, Alier A, Puig L, Santana F, Corvec S (2019) Cutibacterium acnes in primary reverse shoulder arthroplasty: from skin to deep layers. J Shoulder Elb Surg 28:839–846. https://doi.org/10.1016/j.jse.2018.10.016 CrossRef

15.

Crémet L, Bemer P, Zambon O, Reynaud A, Caroff N, Corvec S (2009) Chitinophaga terrae bacteremia in human. Emerg Infect Dis 15:1134–1135. https://doi.org/10.3201/eid1507.090124 CrossRefPubMedPubMedCentral

16.

van Belkum A, Chatellier S, Girard V, Pincus D, Deol P, Dunne WM (2015) Progress in proteomics for clinical microbiology: MALDI-TOF MS for microbial species identification and more. Expert Rev Proteomics 12:595–605. https://doi.org/10.1586/14789450.2015.1091731 CrossRefPubMed

17.

Thomin J, Aubin GG, Foubert F, Corvec S (2015) Assessment of four protocols for rapid bacterial identification from positive blood culture pellets by matrix-assisted laser desorption ionization-time of flight mass spectrometry (Vitek® MS). J Microbiol Methods 115:54–56. https://doi.org/10.1016/j.mimet.2015.05.010 CrossRefPubMed

18.

Girard V, Mailler S, Polsinelli S, Jacob D, Saccomani MC, Celliere B et al (2017) Routine identification of Nocardia species by MALDI-TOF mass spectrometry. Diagn Microbiol Infect Dis 87:7–10. https://doi.org/10.1016/j.diagmicrobio.2016.09.024 CrossRefPubMed

19.

Portillo ME, Corvec S, Borens O, Trampuz A (2013) Propionibacterium acnes: an underestimated pathogen in implant-associated infections. Biomed Res Int 2013:804391. https://doi.org/10.1155/2013/804391 CrossRefPubMedPubMedCentral

20.

Aubin GG, Portillo ME, Trampuz A, Corvec S (2014) Propionibacterium acnes, an emerging pathogen: from acne to implant-infections, from phylotype to resistance. Méd Mal Infect 44:241–250. https://doi.org/10.1016/j.medmal.2014.02.004 CrossRefPubMed

21.

Bémer P, Corvec S, Tariel S, Asseray N, Boutoille D, Langlois C et al (2008) Significance of Propionibacterium acnes-positive samples in spinal instrumentation. Spine 33:E971–E976. https://doi.org/10.1097/BRS.0b013e31818e28dc CrossRefPubMed

22.

Mook WR, Klement MR, Green CL, Hazen KC, Garrigues GE (2015) The incidence of Propionibacterium acnes in open shoulder surgery: a controlled diagnostic study. J Bone Joint Surg Am 97:957–963. https://doi.org/10.2106/JBJS.N.00784 CrossRefPubMed

23.

Eishi Y (2013) Etiologic aspect of sarcoidosis as an allergic endogenous infection caused by Propionibacterium acnes. Biomed Res Int 2013:935289. https://doi.org/10.1155/2013/935289 CrossRefPubMedPubMedCentral

24.

Banzon JM, Rehm SJ, Gordon SM, Hussain ST, Pettersson GB, Shrestha NK (2017) Propionibacterium acnes endocarditis: a case series. Clin Microbiol Infect 2. https://doi.org/10.1016/j.cmi.2016.12.026

25.

Dagnelie M-A, Corvec S, Saint-Jean M, Bourdès V, Nguyen J-M, Khammari A et al (2017) Decrease in diversity of Propionibacterium acnes phylotypes in patients with severe acne on the back. Acta Derm Venereol 14. https://doi.org/10.2340/00015555-2847

26.

Loureiro-Amigo J, Pons S, Sierra M, Meije Y (2016) Prosthetic valve with infective endocarditis caused by Propionibacterium avidum. A case report. Enferm Infecc Microbiol Clin 12. https://doi.org/10.1016/j.eimc.2016.08.010

27.

Kritikos A, Pagin M, Borens O, Voide C, Orasch C (2015) Identification of Propionibacterium avidum from a breast abscess: an overlooked etiology of clinically significant infections. New Microbes New Infect 4:9–10. https://doi.org/10.1016/j.nmni.2014.12.001 CrossRefPubMed

28.

Isabel R, Monica M (2019) Cutibacterium avidum: a rare but expected agent of breast implant infection. IDCases 17:e00546. https://doi.org/10.1016/j.idcr.2019.e00546 CrossRefPubMedPubMedCentral

29.

Tena D, Saa L (2019) Skin and soft tissue infection caused by Cutibacterium (formerly Propionibacterium) avidum: report of eleven cases. Anaerobe 56:91–94. https://doi.org/10.1016/j.anaerobe.2019.02.013 CrossRefPubMed

30.

Lomholt HB, Kilian M (2010) Population genetic analysis of Propionibacterium acnes identifies a subpopulation and epidemic clones associated with acne. PLoS One 5:e12277. https://doi.org/10.1371/journal.pone.0012277 CrossRefPubMedPubMedCentral

31.

Carbonnelle E, Beretti J-L, Cottyn S, Quesne G, Berche P, Nassif X et al (2007) Rapid identification of Staphylococci isolated in clinical microbiology laboratories by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 45:2156–2161. https://doi.org/10.1128/JCM.02405-06 CrossRefPubMedPubMedCentral

32.

Conville PS, Brown-Elliott BA, Smith T, Zelazny AM (2018) The complexities of Nocardia taxonomy and identification. J Clin Microbiol 56. https://doi.org/10.1128/JCM.01419-17

Title: Misidentification of Cutibacterium namnetense as Cutibacterium acnes among clinical isolates by MALDI-TOF VitekMS: usefulness of gyrB sequencing and new player in bone infections
Publication date: 01-08-2020
Keywords: Bone Infection
Acne
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 8/2020
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-020-03873-0

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Misidentification of Cutibacterium namnetense as Cutibacterium acnes among clinical isolates by MALDI-TOF VitekMS: usefulness of gyrB sequencing and new player in bone infections

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