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European Journal of Clinical Microbiology & Infectious Diseases

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01-07-2020 | Meropenem | Original Article

The emergence of carbapenemase bla_NDM genotype among carbapenem-resistant Enterobacteriaceae isolates from Egyptian cancer patients

Authors: Mahmoud M. Tawfick, Walaa A. Alshareef, Hager A. Bendary, Hadir Elmahalawy, Abeer K. Abdulall

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 7/2020

Abstract

Carbapenem resistance among Enterobacteriaceae is a major concern that is increasingly reported worldwide. The objective of this study is to determine the incidence of carbapenem resistance as well as to investigate for carbapenemase-encoding genes among Enterobacteriaceae clinical isolates from cancer patients at different cancer institutes in Egypt. This determination was a cross-sectional study with a total of 135 clinical isolates collected over a period of 1 year. All isolates were sub-cultured on ChromID agar and subjected to phenotypic and molecular detection of carbapenemases. Most of the Enterobacteriaceae isolates were MDR with high resistance rates against tested antimicrobials. Overall, the results of PCR assays revealed that 89.62% (121/135) of isolates harbored one or more of the carbapenemase-encoding genes, while phenotypic assays revealed the production of carbapenemases in 68.88% (93/135) of isolates. BlastN analysis against the non-redundant genome sequences available in the GenBank database revealed that the bla_NDM-1 gene was the most prevalent genotype of carbapenemases in 93/135 (68.88%), followed by bla_OXA-48 44/135 (32.59%), bla_OXA-23 42/135 (31.11%), and bla_KPC-2 2/135 (1.48%). Klebsiella pneumoniae isolates harbored the highest number of carbapenemase-encoding genes 34/121 (28.09%). The high prevalence of carbapenemases and/or their encoding genes among MDR Enterobacteriaceae bacteria in Egypt is alarming, thus, the management of serious infections caused by Enterobacteriaceae, particularly in cancer patients will be challenging to clinicians. Carbapenemase bla_NDM genotype is emerging in cancer healthcare settings in Egypt, which could be the cause of the current increase in carbapenemase-producing Enterobacteriaceae.

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Title: The emergence of carbapenemase blaNDM genotype among carbapenem-resistant Enterobacteriaceae isolates from Egyptian cancer patients
Authors: Mahmoud M. Tawfick
Walaa A. Alshareef
Hager A. Bendary
Hadir Elmahalawy
Abeer K. Abdulall
Publication date: 01-07-2020
Publisher: Springer Berlin Heidelberg
Keywords: Meropenem
Ertapenem
Carbapenem Antibiotic
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 7/2020
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-020-03839-2

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The emergence of carbapenemase bla_NDM genotype among carbapenem-resistant Enterobacteriaceae isolates from Egyptian cancer patients

Abstract

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