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European Journal of Clinical Microbiology & Infectious Diseases

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01-06-2019 | Lymphadenopathy | Original Article

Role of Mycobacterium avium lysate INF-γ, IL-17, and IL-2 ELISPOT assays in diagnosing nontuberculous mycobacteria lymphadenitis in children

Authors: Chiara Della Bella, Elisabetta Venturini, Savannah Devente, Paola Piccini, Simona Tapinassi, Leila Bianchi, Alessia Grassi, Marisa Benagiano, Heba F. Mustafa Alnwaisri, Carlotta Montagnani, Elena Chiappini, Wilbert Bitter, Mario M. D’Elios, Maurizio de Martino, Luisa Galli

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 6/2019

Abstract

Nontuberculous mycobacteria are the most frequent cause of chronic cervical lymphadenitis in childhood. The aim of the study was to evaluate the performance of IL-2, IL-17, and INF-γ in-house enzyme-linked immunospot assays using a Mycobacterium avium lysate, in order to identify a noninvasive diagnostic method of nontuberculous mycobacteria infection. Children with subacute and chronic lymphadenopathies or with a previous diagnosis of nontuberculous mycobacteria lymphadenitis were prospectively enrolled in the study. Sixty children with lymphadenitis were included in our study: 16 with confirmed infection (group 1), 30 probable infected (group 2) and 14 uninfected (group 3). Significantly higher median cytokine values were found in group 1 vs group 2, in group 1 vs group 3, and in group 2 vs group 3 considering IL-2-based enzyme-linked immunospot assay (p = 0.015, p < 0.001, p = 0.004, respectively). INF-γ-based enzyme-linked immunospot assay results were significantly higher in group 2 vs group 3 (p = 0.010). Differences between infected and uninfected children were not significant considering IL-17 assays (p = 0.431). Mycobacterium avium lysate IL-2 and INF-γ-based enzyme-linked immunospot assays seem to be promising noninvasive diagnostic techniques for discriminating children with nontuberculous mycobacteria lymphadenitis and noninfected subjects.

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Title: Role of Mycobacterium avium lysate INF-γ, IL-17, and IL-2 ELISPOT assays in diagnosing nontuberculous mycobacteria lymphadenitis in children
Authors: Chiara Della Bella
Elisabetta Venturini
Savannah Devente
Paola Piccini
Simona Tapinassi
Leila Bianchi
Alessia Grassi
Marisa Benagiano
Heba F. Mustafa Alnwaisri
Carlotta Montagnani
Elena Chiappini
Wilbert Bitter
Mario M. D’Elios
Maurizio de Martino
Luisa Galli
Publication date: 01-06-2019
Publisher: Springer Berlin Heidelberg
Keyword: Lymphadenopathy
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 6/2019
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-019-03506-1

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Role of Mycobacterium avium lysate INF-γ, IL-17, and IL-2 ELISPOT assays in diagnosing nontuberculous mycobacteria lymphadenitis in children

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