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European Journal of Clinical Microbiology & Infectious Diseases
Published in: European Journal of Clinical Microbiology & Infectious Diseases 12/2009

01-12-2009 | Brief Report

High stability of a new Granada medium agar that allows rapid and accurate detection of colonization with group B streptococci in pregnant women

Authors: T. Regnath, R. Ignatius

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 12/2009

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Excerpt

Infections with group B streptococci (GBS, Streptococcus agalactiae) are a leading cause of neonatal morbidity and mortality. CDC have recommended a selective enrichment broth medium with subcultures on sheep blood agar for screening for GBS colonization of pregnant women at 35–37 weeks’ gestation [1]. Granada media (GM) have been suggested for the detection of GBS because S. agalactiae produces a characteristic orange–red pigment. However, the reduced stability of GM has been a major obstacle to their wider laboratory use [2]. We prospectively evaluated a new, commercially available Granada agar that has recently been tested following a distinct methodology [3] for its stability and sensitivity for the detection of GBS. …
Literature
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De la Rosa-Fraile M (2003) Granada agar sensitivity and detection of group B streptococcus. J Clin Microbiol 41(8):4007CrossRef
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Adler A, Block C, Engelstein D, Hochner-Celnikcier D, Drai-Hassid R, Moses AE (2008) Culture-based methods for detection and identification of Streptococcus agalactiae in pregnant women—what are we missing? Eur J Clin Microbiol Infect Dis 27:241–243CrossRefPubMed
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Votava M, Tejkalova M, Drabkova M, Unzeitig V, Braveny I (2001) Use of GBS media for rapid detection of group B streptococci in vaginal and rectal swabs from women in labor. Eur J Clin Microbiol Infect Dis 20(2):120–122CrossRefPubMed
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Gupta C, Briski LE (2004) Comparison of two culture media and three sampling techniques for sensitive and rapid screening of vaginal colonization by group B streptococcus in pregnant women. J Clin Microbiol 42(9):3975–3977CrossRefPubMed
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Tazi A, Réglier-Poupet H, Dautezac F, Raymond J, Poyart C (2008) Comparative evaluation of Strepto B ID chromogenic medium and Granada media for the detection of Group B streptococcus from vaginal samples of pregnant women. J Microbiol Methods 73(3):263–265CrossRefPubMed
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Perry JD, Oliver M, Nicholson A, Wright J, Gould FK (2006) Evaluation of a new chromogenic agar medium for isolation and identification of Group B streptococci. Lett Appl Microbiol 43(6):615–618CrossRefPubMed
Metadata
Title
High stability of a new Granada medium agar that allows rapid and accurate detection of colonization with group B streptococci in pregnant women
Authors
T. Regnath
R. Ignatius
Publication date
01-12-2009
Publisher
Springer-Verlag
Published in
European Journal of Clinical Microbiology & Infectious Diseases / Issue 12/2009
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI
https://doi.org/10.1007/s10096-009-0811-8

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