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Clinical Rheumatology

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01-09-2019 | Rheumatoid Arthritis | Original Article

CD147 participates in the activation function of circulating angiogenic T cells in patients with rheumatoid arthritis

Authors: Peng Zhao, Jinlin Miao, Kui Zhang, Zheng Yu, Minghua Lv, Yingming Xu, Xianghui Fu, Qing Han, Ping Zhu

Published in: Clinical Rheumatology | Issue 9/2019

Abstract

Objective

Rheumatoid arthritis (RA) is a chronic inflammatory and angiogenic disease. This study aimed to explore the profiles of circulating angiogenic T cells (Tang cells) and the role of CD147 in Tang cell activation function in RA.

Methods

Samples were obtained from patients with RA and health controls (HC). Then, Tang cells were quantified by flow cytometry (FCM) in the samples from 87 patients with RA and 29 HC. Tang cells were purified by magnetic cell sorting in cell culture–conditioned media, and the phosphorylation signals were determined by FCM. In addition, cytokine levels were assessed by enzyme-linked immunosorbent assay.

Results

The percentage of circulating Tang cells increased and positively correlated with the number of endothelial progenitor cells in the RA group. Further, the percentage of Tang cells was closely related to disease activity, autoantibody positivity, and proangiogenic cytokine levels. Meanwhile, the expression of CD147 on Tang cells increased in patients with RA. CD147 participated in the Akt phosphorylation and VEGF level of the activated Tang cells.

Conclusions

CD147 may play a critical role in regulating VEGF production of activated Tang cells by affecting Akt signaling, which in turn may serve an essential function in angiogenesis and RA pathogenesis.

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Title: CD147 participates in the activation function of circulating angiogenic T cells in patients with rheumatoid arthritis
Authors: Peng Zhao
Jinlin Miao
Kui Zhang
Zheng Yu
Minghua Lv
Yingming Xu
Xianghui Fu
Qing Han
Ping Zhu
Publication date: 01-09-2019
Publisher: Springer London
Keyword: Rheumatoid Arthritis
Published in: Clinical Rheumatology / Issue 9/2019
Print ISSN: 0770-3198
Electronic ISSN: 1434-9949
DOI: https://doi.org/10.1007/s10067-019-04584-4

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CD147 participates in the activation function of circulating angiogenic T cells in patients with rheumatoid arthritis

Abstract

Objective

Methods

Results

Conclusions

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Abstract

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