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Clinical Oral Investigations
Published in: Clinical Oral Investigations 7/2016

01-09-2016 | Original Article

Evaluation of carbon nanotubes functionalized with sodium hyaluronate in the inflammatory processes for oral regenerative medicine applications

Authors: Paulo Antônio Martins-Júnior, Marcos Augusto Sá, Alesandra Corte Reis, Celso Martins Queiroz-Junior, Marcelo Vidigal Caliari, Mauro Martins Teixeira, Luiz Orlando Ladeira, Vanessa Pinho, Anderson José Ferreira

Published in: Clinical Oral Investigations | Issue 7/2016

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Abstract

Objectives

The objectives of this study were to assess the effects of hyaluronic acid (HY), multi-walled carbon nanotubes (MWCNT), and MWCNT functionalized with HY (HY-MWCNT) on the resolution of neutrophilic inflammation in the pleural cavity of LPS-challenged mice and to assess the influence of these materials in the inflammatory process of bone repair of tooth sockets of rats.

Materials and methods

C57Bl/6 mice were intra-pleurally injected with HY, MWCNT, HY-MWCNT, phosphate-buffered saline (PBS), or LPS. The animals were euthanized after 8 and 24 h, and cells were harvested for total and differential cell counting. The tooth sockets of Wistar rats were filled with HY, MWCNT, HY-MWCNT, or blood clot (control). After 1, 3, and 7 days, histological and morphometric analyses evaluated the number of cell nuclei and blood vessels, and bone trabeculae formation in the sockets. Myeloperoxidase (MPO) activity quantified neutrophil accumulation in the sockets.

Results

HY, MWCNT, and HY-MWCNT increased neutrophilic recruitment at 8 h and reduced the inflammatory process at 24 h in the pleural cavity. Histological and morphometric analyses and MPO activity showed no significant differences in the recruitment of inflammatory cells in the tooth sockets. HY increased the number of blood vessels, and HY and HY-MWCNT increased bone trabeculae formation at 7 days of tooth extraction.

Conclusions

HY, MWCNT, and HY-MWCNT resolved the neutrophilic inflammation in the pleural cavity of the mice. However, these materials did not modulate the inflammatory process in the early stages of bone repair of the tooth sockets, thereby excluding this action as a possible mechanism by which these biomaterials accelerate bone repair.

Clinical relevance

HY-MWCNT is capable of accelerating bone repair/regeneration without affecting the inflammatory phase during the bone healing process.
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Metadata
Title
Evaluation of carbon nanotubes functionalized with sodium hyaluronate in the inflammatory processes for oral regenerative medicine applications
Authors
Paulo Antônio Martins-Júnior
Marcos Augusto Sá
Alesandra Corte Reis
Celso Martins Queiroz-Junior
Marcelo Vidigal Caliari
Mauro Martins Teixeira
Luiz Orlando Ladeira
Vanessa Pinho
Anderson José Ferreira
Publication date
01-09-2016
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 7/2016
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-015-1639-5

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