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Published in:

01-09-2016 | Original Article

Transdentinal cytotoxicity of resin-based luting cements to pulp cells

Authors: Lucas da Fonseca Roberti Garcia, Elaine Cristina Voltolini Pontes, Fernanda Gonçalves Basso, Josimeri Hebling, Carlos Alberto de Souza Costa, Diana Gabriela Soares

Published in: Clinical Oral Investigations | Issue 7/2016

Abstract

Objectives

The aim of this study was to evaluate the transdentinal cytotoxicity of components released from different resin-based luting cements to cultured MDPC-23 odontoblast-like cells and human dental pulp cells (HDPCs).

Materials and methods

Artificial pulp chamber (APC)/dentin disc sets were distributed into four groups according to the materials tested (n = 10), as follows: G1, control (no treatment); G2, resin-modified glass-ionomer cement (RelyX Luting 2); G3, self-adhesive resin cement (RelyX U200); and G4, conventional resin cement (RelyX ARC). The materials were applied to the occlusal surfaces (facing up) of the dentin discs adapted to the APCs. The pulpal surfaces of the discs were maintained in contact with culture medium. Then, an aliquot of 400 μL from the extract (culture medium + resin-based components that diffused through dentin) of each luting cement was applied for 24 h to HDPCs or MDPC-23 cells previously seeded in wells of 24-well plates. Cell viability analysis was performed by the MTT assay (1-way ANOVA/Tukey test; α = 5 %).

Results

For MDPC-23 cells, RelyX ARC (G4) and RelyX Luting 2 (G2) caused greater reduction in cell viability compared with the negative control group (P < 0.05). Only the HDPCs exposed to RelyX ARC (G4) extract showed a tendency toward viability decrease (9.3 %); however, the values were statistically similar to those of the control group (G1) (P > 0.05).

Conclusions

In accordance with the safe limits of ISO 10993-5:1999 (E) recommendations, all resin-based luting cements evaluated in this study can be considered as non-toxic to pulp cells.

Clinical relevance

Cytotoxicity of resin-based luting cements is material-dependent, and the different protocols for the application of these dental materials to dentin may interfere with their cytotoxicity.

De Souza Costa CA, Ribeiro APD, Giro EMA, Randall RC, Hebling J (2011) Pulp response after application of two resin modified glass ionomer cements (RMGICs) in deep cavities of prepared human teeth. Dent Mater 27:158–170. doi:10.1016/j.dental.2011.04.002 CrossRef

Costa CA, Teixeira HM, Nascimento AB, Hebling J (2000) Biocompatibility of two current adhesive resins. J Endod 26:512–516. doi:10.1097/00004770-200009000-00006 CrossRefPubMed

De Souza Costa CAS, Nascimento ABL, Teixeira HM (2002) Response of human pulps following acid conditioning and application of a bonding agent in deep cavities. Dent Mater 18:543–551. doi:10.1016/S0109-5641(01)00089-6 CrossRefPubMed

Lanza CRM, de Souza Costa CA, Furlan M, Alécio A, Hebling J (2009) Transdentinal diffusion and cytotoxicity of self-etching adhesive systems. Cell Biol Toxicol 25:533–543. doi:10.1007/s10565-008-9110-x CrossRefPubMed

Lessa FCR, Nogueira I, Huck C, Hebling J, de Souza Costa CA (2010) Transdentinal cytotoxic effects of different concentrations of chlorhexidine gel applied on acid-conditioned dentin substrate. J Biomed Mater Res B Appl Biomater 92:40–47. doi:10.1002/jbm.b.31487 CrossRefPubMed

Lessa FCR, Nogueira I, Vargas FSV, Spolidorio DMP, Hebling J, de Souza Costa CA (2010) Direct and transdentinal antibacterial effects of chlorhexidine. Am J Dent 23:255–259PubMed

Bianchi L, Ribeiro AP, de Oliveira Carrilho MR, Pashley DH, de Souza Costa CA, Hebling J (2013) Transdentinal cytotoxicity of experimental adhesive systems of different hydrophilicity applied to ethanol-saturated dentin. Dent Mater 29:980–990. doi:10.1016/j.dental.2013.07.006 CrossRefPubMedPubMedCentral

de Souza Costa CA, Hebling J, Randall RC (2006) Human pulp response to resin cements used to bond inlay restorations. Dent Mater 22:954–962. doi:10.1016/j.dental.2005.10.007 CrossRefPubMed

Özcan M, Mese A (2012) Adhesion of conventional and simplified resin-based luting cements to superficial and deep dentin. Clin Oral Invest 16:1081–1088. doi:10.1007/s00784-011-0594-z CrossRef

10.

Pashley DH (1994) Dentine permeability and its role in the pathobiology of dentine sensitivity. Arch Oral Biol 39:73S–80S. doi:10.1016/0003-9969(94)90191-0 CrossRefPubMed

11.

Pashley DH (1996) Dynamics of the pulpo-dentin complex. Crit Rev Oral Biol Med 7:104–133. doi:10.1177/10454411960070020101 CrossRefPubMed

12.

Ulker HE, Sengun A (2009) Cytotoxicity evaluation of self-adhesive composite resin cements by dentin barrier test on 3D pulp cells. Eur J Dent 3:120–126PubMedPubMedCentral

13.

Yasuda Y, Inuyama H, Maeda H, Akamine A, Nör JE, Saito T (2008) Cytotoxicity of one-step dentin-bonding agents toward dental pulp and odontoblast-like cells. J Oral Rehabil 35:940–946. doi:10.1111/j.1365-2842.2008.01885.x CrossRefPubMed

14.

Wataha JC (2012) Predicting clinical biological responses to dental materials. Dent Mater 28:23–40. doi:10.1016/j.dental.2011.08.595 CrossRefPubMed

15.

Goldberg M (2008) In vitro and in vivo studies on the toxicity of dental resin components: a review. Clin Oral Investig 12:1–8. doi:10.1007/s00784-007-0162-8 CrossRefPubMed

16.

Oliveira SS, Pugach MK, Hilton JF, Watanabe LG, Marshall SJ, Marshall Jr GW (2003) The influence of the dentin smear layer on adhesion: a self-etching primer vs. a total-etch system. Dent Mater 19:758–767. doi:10.1016/S0109-5641(03)00023-X CrossRefPubMed

17.

Reis A, Grandi V, Carlotto L, Bortoli G, Patzlaff R, Rodrigues Accorinte ML, et al. (2005) Effect of smear layer thickness and acidity of self-etching solutions on early and long-term bond strength to dentin. J Dent 33:549–559. doi:10.1016/j.jdent.2004.12.003 CrossRefPubMed

18.

International Organization for Standardization: ISO 10993-5:1999 (E) (1999) Biological evaluation of medical devices - Part 5: Tests for in vitro cytotoxicity, 2nd edn. ISO, Géneve

19.

Pontes EC, Soares DG, Hebling J, de Souza Costa CA (2014) Cytotoxicity of resin-based luting cements to pulp cells. Am J Dent 27:237–344PubMed

20.

Araujo PHH, Sayer C, Poco JGR, Giudici R (2002) Techniques for reducing residual monomer content in polymers: a review. Polym Eng Sci 42:1442–1448. doi:10.1002/pen.11043 CrossRef

21.

Radovic I, Monticelli F, Goracci C, Vulicevic ZR, Ferrari M (2008) Self-adhesive resin cements: a literature review. J Adhes Dent 10:251–258PubMed

22.

Makkar S, Malhotra N (2013) Self-adhesive resin cements: a new perspective in luting technology. Dent Update 40:758–760PubMed

23.

Eliades G, Vougiouklakis G, Palaghias G (2001) Heterogeneous distribution of single-bottle adhesive monomers in the resin-dentin interdiffusion zone. Dent Mater 17:277–283. doi:10.1016/S0109-5641(00)00082-8 CrossRefPubMed

24.

Cadenaro M, Breschi L, Rueggeberg FA, Agee K, Di Lenarda R, Carrilho M, Tay FR, Pashley DH (2009) Effect of adhesive hydrophilicity and curing time on the permeability of resins bonded to water vs. ethanol-saturated acid-etched dentin. Dent Mater 25:39–47. doi:10.1016/j.dental.2008.05.004 CrossRefPubMed

25.

Ye Q, Park J, Parthasarathy R (2012) Quantitative analysis of aqueous phase composition of model dentin adhesives experiencing phase separation. J Biomed Mater Res B Appl Biomater 100:1086–1092. doi:10.1002/jbm.b.32675 CrossRefPubMedPubMedCentral

26.

Abedin F, Ye Q, Good HJ, Parthasarathy R, Spencer P (2014) Polymerization- and solvent-induced phase separation in hydrophilic-rich dentin adhesive mimic. Acta Biomater 10:3038–3047. doi:10.1016/j.actbio.2014.03.001 CrossRefPubMedPubMedCentral

27.

Gerzina TM, Hume WR (1995) Effect of hydrostatic pressure on the diffusion of monomers through dentin in vitro. J Dent Res 74:369–373. doi:10.1177/00220345950740011301 CrossRefPubMed

28.

Gerzina TM, Hume WR (1996) Diffusion of monomers from bonding resin-resin composite combinations through dentine in vitro. J Dent 24:125–128. doi:10.1016/0300-5712(95)00036-4 CrossRefPubMed

29.

Hamid A, Hume WR (1997) The effect of dentine thickness on diffusion of resin monomers in vitro. J Oral Rehabil 24:20–25. doi:10.1111/j.1365-2842.1997.tb00255.x CrossRefPubMed

30.

de Souza Costa CA, Hebling J, Scheffel DL, Soares DG, Basso FG, Ribeiro AP (2014) Methods to evaluate and strategies to improve the biocompatibility of dental materials and operative techniques. Dent Mater 30:769–784. doi:10.1016/j.dental.2014.04.010 CrossRefPubMed

31.

Sumer E, Deger Y (2011) Contemporary permanent luting agents used in dentistry: a literature review. Int Dent Res 1:26–31. doi:10.5577/intdentres.2011 CrossRef

32.

Reichl FX, Seiss M, Marquardt W, Kleinsasser N, Schweikl H, Kehe K, Hickel R (2008) Toxicity potentiation by H2O2 with components of dental restorative materials on human oral cells. Arch Toxicol 82:21–28. doi:10.1007/s00204-007-0226-1 CrossRefPubMed

33.

Bakopoulou A, Leyhausen G, Volk J, Tsiftsoglou A, Garefis P, Koidis P, Geurtsen W (2011) Effects of HEMA and TEDGMA on the in vitro odontogenic differentiation potential of human pulp stem/progenitor cells derived from deciduous teeth. Dent Mater 27:608–617. doi:10.1016/j.dental.2011.03.002 CrossRefPubMed

34.

Botsali MS, Kuşgöz A, Altintaş SH, Ülker HE, Tanriver M, Kiliç S, Başak F, Ülker M (2014) Residual HEMA and TEGDMA release and cytotoxicity evaluation of resin-modified glass ionomer cement and compomers cured with different light sources. Sci World J 28:218295. doi:10.1155/2014/218295

35.

Barbosa MO, de Carvalho RV, Demarco FF, Ogliari FA, Zanchi CH, Piva E, da Silva AF (2015) Experimental self-etching HEMA-free adhesive systems: cytotoxicity and degree of conversion. J Mater Sci Mater Med 26:5370. doi:10.1007/s10856-014-5370-6 CrossRefPubMed

36.

de Mendonça AA, Souza PP, Hebling J, Costa CA (2007) Cytotoxic effects of hard-setting cements applied on the odontoblast cell line MDPC-23. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 104:e102–e108. doi:10.1016/j.tripleo.2007.05.017 CrossRefPubMed

37.

Ülker HE, Hiller KA, Schweikl H, Seidenader C, Sengun A, Schmalz G (2012) Human and bovine pulp-derived cell reactions to dental resin cements. Clin Oral Investig 16:1571–1578. doi:10.1007/s00784-011-0657-1 CrossRefPubMed

38.

Rueggeberg FA, Craig RG (1988) Correlation of parameters used to estimate monomer conversion in a light-cured composite. J Dent Res 67:932–937. doi:10.1177/00220345880670060801 CrossRefPubMed

39.

Peutzfeldt A, Asmussen E (2000) The effect of postcuring on quantity of remaining double bonds, mechanical properties, and in vitro wear of two resin composites. J Dent 28:447–452. doi:10.1016/S0300-5712(00)00021-X CrossRefPubMed

Title: Transdentinal cytotoxicity of resin-based luting cements to pulp cells
Authors: Lucas da Fonseca Roberti Garcia
Elaine Cristina Voltolini Pontes
Fernanda Gonçalves Basso
Josimeri Hebling
Carlos Alberto de Souza Costa
Diana Gabriela Soares
Publication date: 01-09-2016
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 7/2016
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-015-1630-1

At a glance: The STEP trials

Springer Medicine

Transdentinal cytotoxicity of resin-based luting cements to pulp cells

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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