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Clinical Oral Investigations
Published in: Clinical Oral Investigations 4/2016

01-05-2016 | Original Article

Citrullination in the periodontium—a possible link between periodontitis and rheumatoid arthritis

Authors: Oliver Laugisch, Alicia Wong, Aneta Sroka, Tomasz Kantyka, Joanna Koziel, Klaus Neuhaus, Anton Sculean, Patrick J. Venables, Jan Potempa, Burkhard Möller, Sigrun Eick

Published in: Clinical Oral Investigations | Issue 4/2016

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Abstract

Objectives

The aim of the present study was to assess human and bacterial peptidylarginine deiminase (PAD) activity in the gingival crevicular fluid (GCF) in the context of serum levels of antibodies against citrullinated epitopes in rheumatoid arthritis and periodontitis.

Materials and methods

Human PAD and Porphyromonas gingivalis-derived enzyme (PPAD) activities were measured in the GCF of 52 rheumatoid arthritis (RA) patients (48 with periodontitis and 4 without) and 44 non-RA controls (28 with periodontitis and 16 without). Serum antibodies against citrullinated epitopes were measured by ELISA. Bacteria being associated with periodontitis were determined by nucleic-acid-based methods.

Results

Citrullination was present in 26 (50 %) RA patients and 23 (48 %) controls. PAD and PPAD activities were detected in 36 (69 %) and 30 (58 %) RA patients, respectively, and in 30 (68 %) and 21 (50 %) controls, respectively. PPAD activity was higher in RA and non-RA patients with periodontitis than in those without (p = 0.038; p = 0.004), and was detected in 35 of 59 P. gingivalis-positive samples, and in 16 of 37 P. gingivalis-negative samples in association with high antibody levels against that species.

Conclusions

PAD and PPAD activities within the periodontium are elevated in RA and non-RA patients with periodontitis. PPAD secreted by P. gingivalis residing in epithelial cells may exert its citrullinating activity in distant regions of the periodontium or even distant tissues.

Clinical relevance

In periodontitis, the citrullination of proteins/peptides by human and bacterial peptidylarginine deiminases may generate antibodies after breaching immunotolerance in susceptible individuals.
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Metadata
Title
Citrullination in the periodontium—a possible link between periodontitis and rheumatoid arthritis
Authors
Oliver Laugisch
Alicia Wong
Aneta Sroka
Tomasz Kantyka
Joanna Koziel
Klaus Neuhaus
Anton Sculean
Patrick J. Venables
Jan Potempa
Burkhard Möller
Sigrun Eick
Publication date
01-05-2016
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 4/2016
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-015-1556-7

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