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Clinical Oral Investigations

Published in:

01-03-2015 | Original Article

Photodynamic killing of Enterococcus faecalis in dentinal tubules using mTHPC incorporated in liposomes and invasomes

Authors: Anna Ossmann, Stefan Kranz, Guellmar Andre, Andrea Völpel, Volker Albrecht, Alfred Fahr, Bernd W. Sigusch

Published in: Clinical Oral Investigations | Issue 2/2015

Abstract

Objectives

The present in vitro study investigates the antimicrobial photodynamic efficiency of the photosensitizer 5,10,15,20-tetra(m-hydroxyphenyl)chlorin (mTHPC) incorporated in liposomes (LIP) and highly flexible invasomes (INV) on the endodontopathogenic species Enterococcus faecalis in infected dental root canals.

Materials and methods

A total of 48 root canals were prepared mechanically to file size ISO 50 and inoculated with E. faecalis for 48 h. In the test groups, the infected root canals were subjected to aPDT with either mTHPC linked to LIP or INV. The controls were either incubated with 1 % chlorohexidine gel (CHX, positive control) or root canals were irrigated with normal saline (NaCl, negative control). After treatment all canals were mechanically enlarged (ISO 50-110), and the debris of each filing process was subjected to bacterial culture analysis.

Results

Both mTHPC formulations showed a significant antimicrobial effect. A bacterial reduction by up to 3.6 log-steps was ascertained for INV directly at the root canal wall. aPDT using INV (ISO 60) was more effective than CHX, which caused a decrease in only 1.2 log-steps. It was found that both liposomal mTHPC formulations were capable to suppress E. faecalis inside the dentinal tubules up to 300 μm.

Conclusions

The results show that mTHPC linked to LIP and INV is capable of efficiently reducing E. faecalis in dental root canals.

Clinical relevance

As evidenced, E. faecalis is resistant to several conventional antibacterial treatment measures. In this context, photodynamic treatment with mTHPC delivered by INV is superior to temporary dressing with 1 % CHX gel applied for 24 h.

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Title: Photodynamic killing of Enterococcus faecalis in dentinal tubules using mTHPC incorporated in liposomes and invasomes
Authors: Anna Ossmann
Stefan Kranz
Guellmar Andre
Andrea Völpel
Volker Albrecht
Alfred Fahr
Bernd W. Sigusch
Publication date: 01-03-2015
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 2/2015
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-014-1271-9

At a glance: The STEP trials

Springer Medicine

Photodynamic killing of Enterococcus faecalis in dentinal tubules using mTHPC incorporated in liposomes and invasomes

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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