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01-09-2021 | Original Article

Mkx regulates the orthodontic tooth movement via osteoclast induction

Authors: Takayuki Miyazaki, Ryota Kurimoto, Tomoki Chiba, Takahide Matsushima, Ryo Nakamichi, Hiroki Tsutsumi, Kaho Takada, Lisa Yagasaki, Tomomi Kato, Kana Shishido, Yukiho Kobayashi, Tsutomu Matsumoto, Keiji Moriyama, Hiroshi Asahara

Published in: Journal of Bone and Mineral Metabolism | Issue 5/2021

Abstract

Introduction

The periodontal ligament (PDL) plays an important role in orthodontic tooth movement; however, the underlying molecular mechanism remains unclear. We have previously reported that the Mohawk homeobox (Mkx), a tendon-specific transcription factor, is expressed in the PDL and regulates its homeostasis.

Materials and methods

In the present study, we examined the role of Mkx in orthodontic tooth movement via bone remodeling induced by mechanical stimulation in Mkx-deficient rats, which are widely used as experimental animals for orthodontic force application. Orthodontic tooth movement of the maxillary first molar was performed in 7-week-old male Mkx-deficient rats (n = 4) and wild-type Wistar rats (n = 4) using coil springs for 14 days. Hematoxylin and eosin (H&E) staining and tartrate-resistant acid phosphatase (TRAP) staining were performed to evaluate morphological changes and osteoclasts. Furthermore, changes in the expression of receptor activator nuclear factor-kappa B ligand (RANKL) were demonstrated using immunostaining.

Results

The amount of tooth movement was significantly lower in Mkx-deficient rats than in wild-type rats. The number of TRAP-positive cells was suppressed in Mkx-deficient rats on the compression side.

Conclusion

Orthodontic tooth movement experiments in Mkx-deficient rats suggested that Mkx is involved in osteoclast induction at the alveolar bone surface on the compression side. This study reveals the possibility that Mkx plays a mechanosensory role in orthodontic tooth movement by inducing RANKL expression and osteoclastogenesis.

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Title: Mkx regulates the orthodontic tooth movement via osteoclast induction
Authors: Takayuki Miyazaki
Ryota Kurimoto
Tomoki Chiba
Takahide Matsushima
Ryo Nakamichi
Hiroki Tsutsumi
Kaho Takada
Lisa Yagasaki
Tomomi Kato
Kana Shishido
Yukiho Kobayashi
Tsutomu Matsumoto
Keiji Moriyama
Hiroshi Asahara
Publication date: 01-09-2021
Publisher: Springer Singapore
Published in: Journal of Bone and Mineral Metabolism / Issue 5/2021
Print ISSN: 0914-8779
Electronic ISSN: 1435-5604
DOI: https://doi.org/10.1007/s00774-021-01233-2

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Mkx regulates the orthodontic tooth movement via osteoclast induction

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Results

Conclusion

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Abstract

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