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01-07-2019 | Original Article

Insulin-like growth factor-I is required to maintain muscle volume in adult mice

Authors: Satoshi Nakamura, Yuiko Sato, Tami Kobayashi, Takatsugu Oike, Yosuke Kaneko, Kana Miyamoto, Atsushi Funayama, Akihito Oya, Toru Nishiwaki, Morio Matsumoto, Masaya Nakamura, Arihiko Kanaji, Takeshi Miyamoto

Published in: Journal of Bone and Mineral Metabolism | Issue 4/2019

Abstract

Insulin-like growth factor-I (IGF-I) is a peptide with diverse functions, among them regulation of embryonic development and bone homeostasis. Serum IGF-I levels decline in the elderly; however, IGF-I function in adults has not been clearly defined. Here, we show that IGF-I is required to maintain muscle mass in adults. We crossed Igf-I flox’d and Mx1 Cre mice to yield Mx1 Cre/Igf-I^flox/flox (IGF-I cKO) mice, and deleted Igf-I in adult mice by polyIpolyC injection. We demonstrate that, although serum IGF-I levels significantly decreased after polyIpolyC injection relative to (Igf-I^flox/flox) controls, serum glucose levels were unchanged. However, muscle mass decreased significantly after IGF-I down-regulation, while bone mass remained the same. In IGF-I cKO muscle, expression of anabolic factors such as Eif4e and p70S6K significantly decreased, while expression of catabolic factors MuRF1 and Atrogin-1 was normal and down-regulated, respectively, suggesting that observed muscle mass reduction was due to perturbed muscle metabolism. Our data demonstrate a specific role for IGF-I in maintaining muscle homeostasis in adults.

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Title: Insulin-like growth factor-I is required to maintain muscle volume in adult mice
Authors: Satoshi Nakamura
Yuiko Sato
Tami Kobayashi
Takatsugu Oike
Yosuke Kaneko
Kana Miyamoto
Atsushi Funayama
Akihito Oya
Toru Nishiwaki
Morio Matsumoto
Masaya Nakamura
Arihiko Kanaji
Takeshi Miyamoto
Publication date: 01-07-2019
Publisher: Springer Japan
Published in: Journal of Bone and Mineral Metabolism / Issue 4/2019
Print ISSN: 0914-8779
Electronic ISSN: 1435-5604
DOI: https://doi.org/10.1007/s00774-018-0964-6

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