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Journal of Bone and Mineral Metabolism
Published in: Journal of Bone and Mineral Metabolism 4/2019

01-07-2019 | Original Article

Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators

Authors: Ana Maria Sper Simão, Maytê Bolean, Bruno Zoccaratto Favarin, Ekeveliny Amabile Veschi, Camila Bussola Tovani, Ana Paula Ramos, Massimo Bottini, Rene Buchet, José Luis Millán, Pietro Ciancaglini

Published in: Journal of Bone and Mineral Metabolism | Issue 4/2019

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Abstract

Tissue-nonspecific alkaline phosphatase (TNAP), a glycosylphosphatidylinositol-anchored ectoenzyme present on the membrane of matrix vesicles (MVs), hydrolyzes the mineralization inhibitor inorganic pyrophosphate as well as ATP to generate the inorganic phosphate needed for apatite formation. Herein, we used proteoliposomes harboring TNAP as MV biomimetics with or without nucleators of mineral formation (amorphous calcium phosphate and complexes with phosphatidylserine) to assess the role of the MVs’ membrane lipid composition on TNAP activity by means of turbidity assay and FTIR analysis. We found that TNAP-proteoliposomes have the ability to induce mineralization even in the absence of mineral nucleators. We also found that the addition of cholesterol or sphingomyelin to TNAP-proteoliposomes composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine reduced the ability of TNAP to induce biomineralization. Our results suggest that the lipid microenvironment is essential for the induction and propagation of minerals mediated by TNAP.
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Metadata
Title
Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators
Authors
Ana Maria Sper Simão
Maytê Bolean
Bruno Zoccaratto Favarin
Ekeveliny Amabile Veschi
Camila Bussola Tovani
Ana Paula Ramos
Massimo Bottini
Rene Buchet
José Luis Millán
Pietro Ciancaglini
Publication date
01-07-2019
Publisher
Springer Japan
Published in
Journal of Bone and Mineral Metabolism / Issue 4/2019
Print ISSN: 0914-8779
Electronic ISSN: 1435-5604
DOI
https://doi.org/10.1007/s00774-018-0962-8

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