Top

Published in:

01-07-2020 | Original Article

Generation of recombinant VP3 protein of infectious bursal disease virus in three different expression systems, antigenic analysis of the obtained polypeptides and development of an ELISA test

Authors: Dmitriy A. Shirokov, Valentin A. Manuvera, Olga A. Miroshina, Alexandr S. Dubovoi, Galina N. Samuseva, Margarita E. Dmitrieva, Vassili N. Lazarev

Published in: Archives of Virology | Issue 7/2020

Abstract

Infectious bursal disease virus (IBDV), which infects young chickens, is one of the most important pathogens that harm the poultry industry. Evaluation of the immune status of birds before and after vaccination is of great importance for controlling the disease caused by this virus. Therefore, the development of low-cost and easy-to-manufacture test systems for IBDV antibody detection remains an urgent issue. In this study, three expression systems (bacteria, yeast, and human cells) were used to produce recombinant VP3 protein of IBDV. VP3 is a group-specific antigen and hence may be a good candidate for use in diagnostic tests. Comparison of the antigenic properties of the obtained polypeptides showed that the titres of antibodies raised in chickens against bacteria- or human-cell-derived recombinant VP3 were high, whereas the antibody level against yeast-derived recombinant VP3 was low. The results of an enzyme-linked immunosorbent assay (ELISA) of sera from IBDV-infected chickens demonstrated that the recombinant VP3 produced in E. coli would be the best choice for use in test systems.

Available only for authorised users

Van Den Berg TP (2000) Acute infectious bursal disease in poultry: a review. Avian Pathol 29(3):175–194. https://doi.org/10.1080/03079450050045431 CrossRefPubMed

Müller H, Islam MR, Raue R (2003) Research on infectious bursal disease: the past, the present and the future. Vet Microbiol 97(1–2):153–165. https://doi.org/10.1016/j.vetmic.2003.08.005 CrossRefPubMed

Muller H, Mundt E, Eterradossi N, Islam MR (2012) Current status of vaccines against infectious bursal disease. Avian Pathol 41(2):133–139. https://doi.org/10.1080/03079457.2012.661403 CrossRefPubMed

Bottcher B, Kiselev NA, Stel'mashchuk VY, Perevozchikova NA, Borisov AV, Crowther RA (1997) Three-dimensional structure of infectious bursal disease virus determined by electron cryomicroscopy. J Virol 71(1):325–330CrossRefPubMedPubMedCentral

Müller H, Scholtissek C, Becht H (1979) The genome of infectious bursal disease virus consists of two segments of double-stranded RNA. J Virol 31(3):584–589CrossRefPubMedPubMedCentral

Mundt E, Beyer J, Muller H (1995) Identification of a novel viral protein in infectious bursal disease virus infected cells. J Gen Virol 76(2):437–443. https://doi.org/10.1099/0022-1317-76-2-437 CrossRefPubMed

Azad AA, Barrett SA, Fahey KJ (1985) The characterization and molecular cloning of the double-stranded RNA genome of an Australian strain of infectious bursal disease virus. Virology 143:35–44. https://doi.org/10.1016/0042-6822(85)90094-7 CrossRefPubMed

Birghan C, Mundt E, Gorbalenya AE (2000) A non-canonical lon proteinase lacking the ATPase domain employs the Ser-Lys catalytic dyad to exercise broad control over the life cycle of a double-stranded RNA virus. EMBO J 19(1):114–123. https://doi.org/10.1093/emboj/19.1.114 CrossRefPubMedPubMedCentral

Müller H, Nitschke R (1987) The two segments of the infectious bursal disease virus genome are circularized by a 90,000-Da protein. Virology 159:174–177. https://doi.org/10.1016/0042-6822(87)90363-1 CrossRefPubMed

10.

Maraver A, Oña A, Abaitua F, González D, Clemente R, Ruiz-Díaz JA, Castón JR, Pazos F, Rodriguez JF (2003) The oligomerization domain of VP3, the scaffolding protein of infectious bursal disease virus, plays a critical role in capsid assembly. J Virol 77:6438–6449. https://doi.org/10.1128/jvi.77.11.6438-6449.2003 CrossRefPubMedPubMedCentral

11.

Lombardo E, Maraver A, Castón JR, Rivera J, Fernández-Arias A, Serrano A, Carrascosa JL, Rodriguez JF (1999) VP1, the putative RNA-dependent RNA polymerase of infectious bursal disease virus, forms complexes with the capsid protein VP3, leading to efficient encapsidation into virus-like particles. J Virol 73:6973–6983. https://doi.org/10.1128/JVI.73.8.6973-6983.1999 CrossRefPubMedPubMedCentral

12.

Luque D, Saugar I, Rejas MT, Carrascosa JL, Rodríguez JF, Castón JR (2009) Infectious Bursal disease virus: ribonucleoprotein complexes of a double-stranded RNA virus. J Mol Biol 386:891–901. https://doi.org/10.1016/j.jmb.2008.11.029 CrossRefPubMed

13.

Dalton RM, Rodrıguez JF (2014) Rescue of Infectious Birnavirus from Recombinant Ribonucleoprotein Complexes. PLoS ONE 9(1):e87790. https://doi.org/10.1371/journal.pone.0087790 CrossRefPubMedPubMedCentral

14.

Busnadiego I, Maestre AM, Rodriguez D, Rodriguez JF (2012) The infectious bursal disease virus RNA-binding VP3 polypeptide inhibits PKR-mediated apoptosis. PLoS ONE 7:e46768. https://doi.org/10.1371/journal.pone.0046768 CrossRefPubMedPubMedCentral

15.

Valli A, Busnadiego I, Maliogka V, Ferrero D, Castón JR, Rodríguez JF, García JA (2012) The VP3 factor from viruses of Birnaviridae family suppresses RNA silencing by binding both long and small RNA duplexes. PLoS ONE 7:e45957. https://doi.org/10.1371/journal.pone.0045957 CrossRefPubMedPubMedCentral

16.

Fahey KJ, O’Donnell IJ, Azad AA (1985) Characterization by Western blotting of the immunogens of infectious bursal disease virus. J Gen Virol 66:1479–1488. https://doi.org/10.1099/0022-1317-66-7-1479 CrossRefPubMed

17.

Becht H, Muller H, Muller HK (1988) Comparative studies on structural and antigenic properties of two serotypes of infectious bursal disease virus. J Gen Virol 69:631–640. https://doi.org/10.1099/0022-1317-69-3-631 CrossRefPubMed

18.

Jackwood DJ, Henderson KS, Jackwood RJ (1996) Enzyme-linked immunosorbent assay-based detection of antibodies to antigenic subtypes of infectious bursal disease viruses of chickens. Clin Diagn Lab Immunol 3(4):456–463CrossRefPubMedPubMedCentral

19.

Wang MY, Hu HL, Suen SY, Chiu FY, Shien JH, Lai SY (2008) Development of an enzyme-linked immunosorbent assay for detecting infectious bursal disease virus (IBDV) infection based on the VP3 structural protein. Vet Microbiol 131(3–4):229–236. https://doi.org/10.1016/j.vetmic.2008.03.010 CrossRefPubMed

20.

Singh NK, Dey S, Madhan Mohan C, Mohan Kataria J, Vakharia VN (2010) Evaluation of four enzyme linked immunosorbent assays for the detection of antibodies to infectious bursal disease in chickens. J Virol Methods 165(2):277–282. https://doi.org/10.1016/j.jviromet.2010.02.008 CrossRefPubMed

21.

Martinez-Torrecuadrada JL, Lazaro B, Rodriguez JF, Casal JI (2000) Antigenic properties and diagnostic potential of baculovirus-expressed infectious bursal disease virus proteins VPX and VP3. Clin Diagn Lab Immunol 7:645–651. https://doi.org/10.1128/cdli.7.4.645-651.2000 CrossRefPubMedPubMedCentral

22.

Shirokov DA, Dubovoi AS, Manuvera VA, Samuseva GN, Dmitrieva ME, Lazarev VN (2018) Complete genome sequence of a novel very virulent strain of infectious bursal disease virus circulating in Russia. Microbiol Resour Announc 7:e01084–e1118. https://doi.org/10.1128/MRA.01084-18 CrossRefPubMedPubMedCentral

23.

Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685. https://doi.org/10.1038/227680a0 CrossRefPubMed

24.

Classen DC, Morningstar JM, Shanley JD (1987) Detection of antibody to murine cytomegalovirus by enzyme-linked immunosorbent and indirect immunofluorescence assays. J Clin Microbiol 25(4):600–604CrossRefPubMedPubMedCentral

25.

Boonham N, Kreuze J, Winter S, van der Vlugt R, Bergervoet J, Tomlinson J, Mumford R (2014) Methods in virus diagnostics: From ELISA to next generation sequencing. Virus Res 186:20–31. https://doi.org/10.1016/j.virusres.2013.12.007 CrossRefPubMed

26.

de Wit JJ, van de Sande HW, Counotte GH, Wellenberg GJ (2007) Analyses of the results of different test systems in the 2005 global proficiency testing schemes for infectious bursal disease virus and Newcastle disease virus antibody detection in chicken serum. Avian Pathol 36(2):177–183. https://doi.org/10.1080/03079450601105676 CrossRefPubMed

27.

Snyder DB, Marquardt WW, Mallinson ET, Russek-Cohen E, Savage PK, Allen DC (1986) Rapid serological profiling by enzyme-linked immunosorbent assay. IV. Association of infectious bursal disease serology with broiler flock performance. Avian Dis 30(1):139–148CrossRefPubMed

28.

Macauley-Patrick S, Fazenda ML, McNeil B, Harvey LM (2005) Heterologous protein production using the Pichia pastoris expression system. Yeast 22:249–270. https://doi.org/10.1002/yea.1208 CrossRefPubMed

29.

VIOLIN, vaccine database: https://www.violinet.org/vaxquery/vaxquery_results.php?searchEngine=vaxquery&keywords=IBDV

30.

Bayliss CD, Peters RW, Cook JK, Reece RL, Howes K, Binns MM, Boursnell ME (1991) A recombinant fowlpox virus that expresses the VP2 antigen of infectious bursal disease virus induces protection against mortality caused by the virus. Arch Virol 120(3–4):193–205. https://doi.org/10.1007/BF01310475 CrossRefPubMed

31.

Shaw I, Davison TF (2000) Protection from IBDV-induced bursal damage by a recombinant fowlpox vaccine, fpIBD1, is dependent on the titre of challenge virus and chicken genotype. Vaccine 18(28):3230–3241. https://doi.org/10.1016/s0264-410x(00)00133-x CrossRefPubMed

32.

Darteil R, Bublot M, Laplace E, Bouquet JF, Audonnet JC, Rivière M (1995) Herpesvirus of turkey recombinant viruses expressing infectious bursal disease virus (IBDV) VP2 immunogen induce protection against an IBDV virulent challenge in chickens. Virology 211(2):481–490. https://doi.org/10.1006/viro.1995.1430 CrossRefPubMed

Title: Generation of recombinant VP3 protein of infectious bursal disease virus in three different expression systems, antigenic analysis of the obtained polypeptides and development of an ELISA test
Authors: Dmitriy A. Shirokov
Valentin A. Manuvera
Olga A. Miroshina
Alexandr S. Dubovoi
Galina N. Samuseva
Margarita E. Dmitrieva
Vassili N. Lazarev
Publication date: 01-07-2020
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 7/2020
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-020-04650-2

ACC 2024 Congress Coverage

Heart Failure Video

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Generation of recombinant VP3 protein of infectious bursal disease virus in three different expression systems, antigenic analysis of the obtained polypeptides and development of an ELISA test

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 7/2020

Isolation and identification of two new strains of mammalian orthoreovirus from Chinese tree shrews

A new begomovirus isolated from a potyvirus-infected bean plant causes asymptomatic infections in bean and N. benthamiana

Molecular characterization of a novel mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea

Epidemiological and genetic characteristics of porcine reproduction and respiratory syndrome virus 2 in mainland China, 2017–2018

Complete genome sequence of an isolate of duck enteritis virus from China

Genomic characterization of classical swine fever virus LOM variants with 3′-UTR INDELs from pigs on Jeju Island, South Korea

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page