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Published in: Archives of Virology 6/2020

01-06-2020 | Hepatitis B | Original Article

Intra-host diversity of hepatitis B virus during mother-to-child transmission: the X gene may play a key role in virus survival in children after transmission

Authors: Yanjie Li, Chenguang Shen, Liuqing Yang, Yang Yang, Miao Wang, Shanqin Li, Feng Chen, Min Yang, Ling Peng, Jinmin Ma, Zhongping Duan, Liqiang Li, Yingxia Liu

Published in: Archives of Virology | Issue 6/2020

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Abstract

Mother-to-child transmission of hepatitis B virus (HBV) is the main route of transmission in Asia, and characterization of HBV quasispecies is needed to further understand virus evolution and adaptation. To understand changes in HBV during mother-to-child transmission, we enrolled nine pairs of mothers and children in the study, including a set of twins. Three groups were infected with HBV genotype C, and six groups were infected with HBV genotype B. The full-length HBV genome was amplified by PCR from serum samples before antiviral treatment, the whole viral genomes from each pair were sequenced, and the complexity and diversity of the quasispecies were analyzed. The entropy of transmitted HBV in children was found to be lower than their mothers, suggesting that there was a bottleneck effect during HBV transmission from the mother to the child. Selective evolution was shown by calculating πN and πS in the whole genomes, and the highest values were obtained for the X gene, which plays a role in viral replication and immune escape. All genotype C patients and only one genotype B pair had a πN/πS greater than 1 ratio, indicating that positive selection had occurred. In addition, quasispecies were found to be different between the twin children despite having the same mother, indicating that virus evolution is host-specific.
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Metadata
Title
Intra-host diversity of hepatitis B virus during mother-to-child transmission: the X gene may play a key role in virus survival in children after transmission
Authors
Yanjie Li
Chenguang Shen
Liuqing Yang
Yang Yang
Miao Wang
Shanqin Li
Feng Chen
Min Yang
Ling Peng
Jinmin Ma
Zhongping Duan
Liqiang Li
Yingxia Liu
Publication date
01-06-2020
Publisher
Springer Vienna
Keyword
Hepatitis B
Published in
Archives of Virology / Issue 6/2020
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-020-04597-4

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