Top

Published in:

01-03-2020 | Brief Report

Development and application of an indirect ELISA for the serological detection of duck Tembusu virus infection based on the NS1 protein antigen

Authors: Qi Zhou, Zhuangli Bi, Dongdong Yin, Xiangxue Gu, Zejun Xu, Rong Huang, Xue Xing, Kezong Qi, Guijun Wang

Published in: Archives of Virology | Issue 3/2020

Abstract

Duck Tembusu virus (DTMUV) has caused significant economic losses in China since 2010. However, there is still a lack of effective methods to diagnose the disease caused by this virus, and especially to differentiate infection from vaccination. In this study, we established a novel indirect enzyme-linked immunosorbent assay (iELISA) and performed a retrospective serological survey for DTMUV in Anhui province, China. Our results show that the iELISA displayed high specificity sensitivity, and with no serological cross-reaction with other duck pathogens. These findings indicate that the newly developed iELISA could be a useful screening tool for large-scale monitoring of the epidemiology of DTMUV infection in ducks.

Available only for authorised users

Bai X, Shaozhou W, Zhang Q, Li C, Qiu N, Meng R, Liu M, Zhang Y (2015) Characterization of monoclonal antibodies against duck Tembusu virus E protein: an antigen-capture ELISA for the detection of Tembusu virus infection. Arch Virol 160:757–764CrossRef

Cao Z, Zhang C, Liu Y, Liu Y, Ye W, Han J, Ma G, Zhang D, Xu F, Gao X, Tang Y, Shi S, Wan C, Zhang C, He B, Yang M, Lu X, Huang Y, Diao Y, Ma X, Zhang D (2011) Tembusu virus in ducks, china. Emerg Infect Dis 17:1873–1875CrossRef

Fu Y, Ji Y, Liu B, Dafallah RM, Zhu Q (2015) Development of a solid-phase competition ELISA to detect antibodies against newly emerged Duck Tembusu virus. J Virol Methods 224:73–76CrossRef

Gelanew T, Hunsperger E (2018) Development and characterization of serotype-specific monoclonal antibodies against the dengue virus-4 (DENV-4) non-structural protein (NS1). Virol J 15:30CrossRef

Han K, Zhao D, Liu Y, Liu Q, Huang X, Yang J, Bi K, Xu T, Li Y (2016) Generation and characterization of a monoclonal antibody against duck Tembusu virus envelope protein. Pol J Vet Sci 19:877–883CrossRef

Hang VT, Nguyet NM, Trung DT, Tricou V, Yoksan S, Dung NM, Van Ngoc T, Hien TT, Farrar J, Wills B, Simmons CP (2009) Diagnostic accuracy of NS1 ELISA and lateral flow rapid tests for dengue sensitivity, specificity and relationship to viraemia and antibody responses. PLoS Negl Trop Dis 3:e360CrossRef

Homonnay ZG, Kovacs EW, Banyai K, Albert M, Feher E, Mato T, Tatar-Kis T, Palya V (2014) Tembusu-like flavivirus (Perak virus) as the cause of neurological disease outbreaks in young Pekin ducks. Avian Pathol J WVPA 43:552–560CrossRef

Kitai Y, Shoda M, Kondo T, Konishi E (2007) Epitope-blocking enzyme-linked immunosorbent assay to differentiate west nile virus from Japanese encephalitis virus infections in equine sera. Clin Vaccine Immunol CVI 14:1024–1031CrossRef

Konishi E, Shoda M, Ajiro N, Kondo T (2004) Development and evaluation of an enzyme-linked immunosorbent assay for quantifying antibodies to Japanese encephalitis virus nonstructural 1 protein to detect subclinical infections in vaccinated horses. J Clin Microbiol 42:5087–5093CrossRef

10.

Li N, Lv C, Yue R, Shi Y, Wei L, Chai T, Liu S (2015) Effect of age on the pathogenesis of duck tembusu virus in Cherry Valley ducks. Front Microbiol 6:581PubMedPubMedCentral

11.

Li X, Li G, Teng Q, Yu L, Wu X, Li Z (2012) Development of a blocking ELISA for detection of serum neutralizing antibodies against newly emerged duck Tembusu virus. PLoS One 7:e53026CrossRef

12.

Lim CS, Krishnan G, Sam CK, Ng CC (2013) On optimizing the blocking step of indirect enzyme-linked immunosorbent assay for Epstein-Barr virus serology. Clin Chim Acta Int J Clin Chem 415:158–161CrossRef

13.

Liu P, Lu H, Li S, Wu Y, Gao GF, Su J (2013) Duck egg drop syndrome virus: an emerging Tembusu-related flavivirus in China. Sci China Life Sci 56:701–710CrossRef

14.

Macdonald J, Tonry J, Hall RA, Williams B, Palacios G, Ashok MS, Jabado O, Clark D, Tesh RB, Briese T, Lipkin WI (2005) NS1 protein secretion during the acute phase of West Nile virus infection. J Virol 79:13924–13933CrossRef

15.

Shen HQ, Lin WC, Wang ZX, Zhang K, Yan ZQ, Zhou QF, Qin JP, Xie QM, Bi YZ, Chen F (2016) Pathogenicity and genetic characterization of a duck Tembusu virus associated with egg-dropping in Muscovy ducks. Virus Res 223:52–56CrossRef

16.

Shu PY, Chen LK, Chang SF, Yueh YY, Chow L, Chien LJ, Chin C, Lin TH, Huang JH (2001) Antibody to the nonstructural protein NS1 of Japanese encephalitis virus: potential application of mAb-based indirect ELISA to differentiate infection from vaccination. Vaccine 19:1753–1763CrossRef

17.

Shu PY, Chen LK, Chang SF, Yueh YY, Chow L, Chien LJ, Chin C, Lin TH, Huang JH (2003) Comparison of capture immunoglobulin M (IgM) and IgG enzyme-linked immunosorbent assay (ELISA) and nonstructural protein NS1 serotype-specific IgG ELISA for differentiation of primary and secondary dengue virus infections. Clin Diagn Lab Immunol 10:622–630PubMedPubMedCentral

18.

Tang Y, Gao X, Diao Y, Feng Q, Chen H, Liu X, Ge P, Yu C (2013) Tembusu virus in human, China. Transbound Emerg Dis 60:193–196CrossRef

19.

Thontiravong A, Ninvilai P, Tunterak W, Nonthabenjawan N, Chaiyavong S, Angkabkingkaew K, Mungkundar C, Phuengpho W, Oraveerakul K, Amonsin A (2015) Tembusu-related flavivirus in ducks, Thailand. Emerg Infect Dis 21:2164–2167CrossRef

20.

Yan L, Yan P, Zhou J, Teng Q, Li Z (2011) Establishing a TaqMan-based real-time PCR assay for the rapid detection and quantification of the newly emerged duck Tembusu virus. Virol J 8:464CrossRef

21.

Yan L, Peng S, Yan P, Zhou J, Teng Q, Li G, Li X, Li Z (2012) Comparison of real-time reverse transcription loop-mediated isothermal amplification and real-time reverse transcription polymerase chain reaction for duck Tembusu virus. J Virol Methods 182:50–55CrossRef

22.

Yan P, Zhao Y, Zhang X, Xu D, Dai X, Teng Q, Yan L, Zhou J, Ji X, Zhang S, Liu G, Zhou Y, Kawaoka Y, Tong G, Li Z (2011) An infectious disease of ducks caused by a newly emerged Tembusu virus strain in mainland China. Virology 417:1–8CrossRef

23.

Yan Z, Shen H, Wang Z, Lin W, Xie Q, Bi Y, Chen F (2017) Isolation and characterization of a novel tembusu virus circulating in muscovy ducks in South China. Transbound Emerg Dis 64:e15–e17CrossRef

24.

Yin X, Lv R, Chen X, Liu M, Hua R, Zhang Y (2013) Detection of specific antibodies against tembusu virus in ducks by use of an E protein-based enzyme-linked immunosorbent assay. J Clin Microbiol 51:2400–2402CrossRef

25.

Young PR, Hilditch PA, Bletchly C, Halloran W (2000) An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol 38:1053–1057CrossRef

26.

Yu G, Lin Y, Tang Y, Diao Y (2018) Evolution of Tembusu virus in ducks, chickens, geese, sparrows, and mosquitoes in Northern China. Viruses 10:485CrossRef

27.

Yun T, Ni Z, Hua J, Ye W, Chen L, Zhang S, Zhang Y, Zhang C (2012) Development of a one-step real-time RT-PCR assay using a minor-groove-binding probe for the detection of duck Tembusu virus. J Virol Methods 181:148–154CrossRef

28.

Yun T, Ye W, Ni Z, Zhang D, Zhang C (2012) Identification and molecular characterization of a novel flavivirus isolated from Pekin ducklings in China. Vet Microbiol 157:311–319CrossRef

29.

Zhou X, Zhang T, Song D, Huang T, Peng Q, Chen Y, Li A, Zhang F, Wu Q, Ye Y, Tang Y (2016) Whole-Genome Sequence of Duck Tembusu Virus Strain DTMUV/CH/2014, Isolated in China. Genome Announc 4:e01657-15CrossRef

30.

Zhu W, Chen J, Wei C, Wang H, Huang Z, Zhang M, Tang F, Xie J, Liang H, Zhang G, Su S (2012) Complete genome sequence of duck Tembusu virus, isolated from Muscovy ducks in southern China. J Virol 86:13119CrossRef

Title: Development and application of an indirect ELISA for the serological detection of duck Tembusu virus infection based on the NS1 protein antigen
Authors: Qi Zhou
Zhuangli Bi
Dongdong Yin
Xiangxue Gu
Zejun Xu
Rong Huang
Xue Xing
Kezong Qi
Guijun Wang
Publication date: 01-03-2020
Publisher: Springer Vienna
Published in: Archives of Virology / Issue 3/2020
Print ISSN: 0304-8608
Electronic ISSN: 1432-8798
DOI: https://doi.org/10.1007/s00705-019-04495-4

ACC 2024 Congress Coverage

Heart Failure Video

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Development and application of an indirect ELISA for the serological detection of duck Tembusu virus infection based on the NS1 protein antigen

Abstract

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2020

Transition in genetic constellations of H3N8 and H4N6 low-pathogenic avian influenza viruses isolated from an overwintering site in Japan throughout different winter seasons

A new polycipivirus identified in Colobopsis shohki

The pattern of antiviral protein expression induced by interferon λ1 in peripheral blood mononuclear cells of patients with chronic hepatitis C virus infection

The small molecule AZD6244 inhibits dengue virus replication in vitro and protects against lethal challenge in a mouse model

Characterization of a novel echovirus 21 strain isolated from a healthy child in China in 2013

Genetic diversity of group A rotaviruses in Moscow in 2018-2019

ACC 2024 Congress Coverage

Year in Review: Heart failure and cardiomyopathies

Semaglutide benefits people with type 2 diabetes and obesity-related heart failure

Incentivised to exercise

New intervention for STEMI-related cardiogenic shock

» View more highlights on the ACC 2024 congress hub page